LOCUS       BBa_K318503              704 bp    DNA     linear       02-Jan-2026
DEFINITION  lacI pL + RBS + YgiV + TT
ACCESSION   BBa_K318503
VERSION     BBa_K318503.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=start
     CDS             82..561
                     /label=cds
     misc_feature    562..564
                     /label=stop
     misc_feature    565..567
                     /label=stop
     CDS             82..567
                     /label=BBa_K200002
     stem_loop       587..630
                     /label=stem_loop
     misc_feature    576..655
                     /label=BBa_B0010
     terminator      576..655
                     /label=BBa_B0010
     stem_loop       671..690
                     /label=T7 TE
     misc_feature    697..697
                     /label=stop
     polyA_site      691..704
                     /label=polya
     misc_feature    664..704
                     /label=BBa_B0012
     terminator      664..704
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgacaaac ctgacactgg atgtaaacat tatcgatttc
      121 ccatcaatac ctgtggcgat gttgccgcac cgctgtagcc ctgaattgct caactacagc
      181 gtggcgaaat ttatcatgtg gcgtaaagag acggggcttt ctcctgttaa ccaaagccag
      241 acttttggcg tcgcctggga cgaccctgcc accaccgcac cggaagcgtt tcgctttgat
      301 atctgcggca gcgttagcga accgattccc gataatcgtt atggtgtgag caatggtgaa
      361 cttaccggtg gacgttatgc cgtggcccgc cacgttggcg agctggacga tatttcacac
      421 acggtatggg gcatcattcg ccactggctg cctgcaagcg gcgagaaaat gcgtaaagca
      481 ccgattctgt ttcactacac caatcttgcc gaaggggtga cagagcagcg actggaaacg
      541 gatgtttatg tgccgttggc gtgataatac tagagccagg catcaaataa aacgaaaggc
      601 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      661 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//