LOCUS       BBa_K318504             1481 bp    DNA     linear       15-Nov-2024
DEFINITION  lacI pL + RBS + RfaI + TT
ACCESSION   BBa_K318504
VERSION     BBa_K318504.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1338
                     /label=Waa Ligase
     misc_feature    82..84
                     /label=start
     misc_feature    1339..1341
                     /label=stop
     misc_feature    1342..1344
                     /label=stop
     CDS             82..1344
                     /label=BBa_K200003
     stem_loop       1364..1407
                     /label=stem_loop
     misc_feature    1353..1432
                     /label=BBa_B0010
     terminator      1353..1432
                     /label=BBa_B0010
     stem_loop       1448..1467
                     /label=T7 TE
     misc_feature    1474..1474
                     /label=stop
     polyA_site      1468..1481
                     /label=polya
     misc_feature    1441..1481
                     /label=BBa_B0012
     terminator      1441..1481
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgctaaca tcctttaaac ttcattcatt gaaaccttac
      121 actctgaaat catcaatgat tttagagata ataacttata tattatgttt tttttcaatg
      181 ataattgcat tcgtcgataa tactttcagc ataaaaatat ataatatcac tgctatagtt
      241 tgcttattgt cactaatttt acgtggcaga caagaaaatt ataatataaa aaaccttatt
      301 cttccccttt ctatattttt aataggcttg cttgatttaa tttggtattc tgcgtttaaa
      361 gtagataatt cgccatttcg tgctacttac catagttatt taaatactgc caaaatattt
      421 atatttggtt cttttattgt tttcttgaca ctaactagcc agctaaaatc aaaaaaagag
      481 agtgtattat acactttgta ttctctgtca tttctaattg ctggatatgc aatgtatatt
      541 aatagcattc atgaaaatga ccgcatttct tttggtgtag gaacggcaac aggagcagca
      601 tattcaacaa tgctaatagg gatagttagt ggcgttgcga ttctttatac taagaaaaat
      661 catccttttt tatttttatt aaatagttgc gcggtacttt atgttctggc gctaacacaa
      721 accagagcaa ccctactcct gttccctata atttgtgttg ctgcattaat agcttattat
      781 aataaatcac ccaagaaatt cacttcctct attgttctac taattgctat attagctagc
      841 attgttatta tatttaataa accaatacag aatcgctata atgaagcatt aaatgactta
      901 aacagttata ccaatgctaa tagtgttact tccctaggtg caagactggc aatgtacgaa
      961 attggtttaa atatattcat aaagtcacct ttttcattta gatcagcaga gtcacgcgct
     1021 gaaagtatga atttgttagt tgcagaacac aataggctaa gaggggcatt ggagttttct
     1081 aacgtacatc tacataatga gataattgaa gcagggtcac tgaaaggtct gatgggaatt
     1141 ttttccacac ttttcctcta tttttcacta ttttatatag catataaaaa acgagctttg
     1201 ggtttgttga tattaacgct tggcattgtg gggattggac tcagtgatgt gatcatatgg
     1261 gcacgcagca ttccaattat cattatatcc gctatagtcc tcttactcgt cattaataat
     1321 cgtaacaata caattaatta ataatactag agccaggcat caaataaaac gaaaggctca
     1381 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1441 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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