LOCUS       BBa_K318505             1993 bp    DNA     linear       16-Dec-2024
DEFINITION  lacI pL + RBS + YgiV + RBS + RfaI + TT
ACCESSION   BBa_K318505
VERSION     BBa_K318505.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=start
     CDS             82..561
                     /label=cds
     misc_feature    562..564
                     /label=stop
     misc_feature    565..567
                     /label=stop
     CDS             82..567
                     /label=BBa_K200002
     misc_feature    580..583
                     /label=conserved
     RBS             576..587
                     /label=BBa_B0034
     CDS             594..1850
                     /label=Waa Ligase
     misc_feature    594..596
                     /label=start
     misc_feature    1851..1853
                     /label=stop
     misc_feature    1854..1856
                     /label=stop
     CDS             594..1856
                     /label=BBa_K200003
     stem_loop       1876..1919
                     /label=stem_loop
     misc_feature    1865..1944
                     /label=BBa_B0010
     terminator      1865..1944
                     /label=BBa_B0010
     stem_loop       1960..1979
                     /label=T7 TE
     misc_feature    1986..1986
                     /label=stop
     polyA_site      1980..1993
                     /label=polya
     misc_feature    1953..1993
                     /label=BBa_B0012
     terminator      1953..1993
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgacaaac ctgacactgg atgtaaacat tatcgatttc
      121 ccatcaatac ctgtggcgat gttgccgcac cgctgtagcc ctgaattgct caactacagc
      181 gtggcgaaat ttatcatgtg gcgtaaagag acggggcttt ctcctgttaa ccaaagccag
      241 acttttggcg tcgcctggga cgaccctgcc accaccgcac cggaagcgtt tcgctttgat
      301 atctgcggca gcgttagcga accgattccc gataatcgtt atggtgtgag caatggtgaa
      361 cttaccggtg gacgttatgc cgtggcccgc cacgttggcg agctggacga tatttcacac
      421 acggtatggg gcatcattcg ccactggctg cctgcaagcg gcgagaaaat gcgtaaagca
      481 ccgattctgt ttcactacac caatcttgcc gaaggggtga cagagcagcg actggaaacg
      541 gatgtttatg tgccgttggc gtgataatac tagagaaaga ggagaaatac tagatgctaa
      601 catcctttaa acttcattca ttgaaacctt acactctgaa atcatcaatg attttagaga
      661 taataactta tatattatgt tttttttcaa tgataattgc attcgtcgat aatactttca
      721 gcataaaaat atataatatc actgctatag tttgcttatt gtcactaatt ttacgtggca
      781 gacaagaaaa ttataatata aaaaacctta ttcttcccct ttctatattt ttaataggct
      841 tgcttgattt aatttggtat tctgcgttta aagtagataa ttcgccattt cgtgctactt
      901 accatagtta tttaaatact gccaaaatat ttatatttgg ttcttttatt gttttcttga
      961 cactaactag ccagctaaaa tcaaaaaaag agagtgtatt atacactttg tattctctgt
     1021 catttctaat tgctggatat gcaatgtata ttaatagcat tcatgaaaat gaccgcattt
     1081 cttttggtgt aggaacggca acaggagcag catattcaac aatgctaata gggatagtta
     1141 gtggcgttgc gattctttat actaagaaaa atcatccttt tttattttta ttaaatagtt
     1201 gcgcggtact ttatgttctg gcgctaacac aaaccagagc aaccctactc ctgttcccta
     1261 taatttgtgt tgctgcatta atagcttatt ataataaatc acccaagaaa ttcacttcct
     1321 ctattgttct actaattgct atattagcta gcattgttat tatatttaat aaaccaatac
     1381 agaatcgcta taatgaagca ttaaatgact taaacagtta taccaatgct aatagtgtta
     1441 cttccctagg tgcaagactg gcaatgtacg aaattggttt aaatatattc ataaagtcac
     1501 ctttttcatt tagatcagca gagtcacgcg ctgaaagtat gaatttgtta gttgcagaac
     1561 acaataggct aagaggggca ttggagtttt ctaacgtaca tctacataat gagataattg
     1621 aagcagggtc actgaaaggt ctgatgggaa ttttttccac acttttcctc tatttttcac
     1681 tattttatat agcatataaa aaacgagctt tgggtttgtt gatattaacg cttggcattg
     1741 tggggattgg actcagtgat gtgatcatat gggcacgcag cattccaatt atcattatat
     1801 ccgctatagt cctcttactc gtcattaata atcgtaacaa tacaattaat taataatact
     1861 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1921 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1981 ttctgcgttt ata
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