##gff-version 3
##sequence-region BBa_K318505 1 1993
BBa_K318505	.	CDS	82	567	.	+	0	ID=BBa_K200002;Name=BBa_K200002
BBa_K318505	.	stop_codon	562	564	.	+	0	ID=annotation2018132;Name=stop;Parent=BBa_K200002
BBa_K318505	.	stop_codon	565	567	.	+	0	ID=annotation2027405;Name=stop;Parent=BBa_K200002
BBa_K318505	.	CDS	82	561	.	+	0	ID=annotation2018131;Name=cds;Parent=BBa_K200002
BBa_K318505	.	start_codon	82	84	.	+	0	ID=annotation2018130;Name=start;Parent=BBa_K200002
BBa_K318505	.	ribosome_entry_site	576	587	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K318505	.	conserved_region	580	583	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K318505	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K318505	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K318505	.	terminator	1865	1944	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K318505	.	stem_loop	1876	1919	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K318505	.	engineered_region	1865	1944	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K318505	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K318505	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K318505	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K318505	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K318505	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K318505	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K318505	.	CDS	594	1856	.	+	0	ID=BBa_K200003;Name=BBa_K200003
BBa_K318505	.	start_codon	594	596	.	+	0	ID=annotation2018141;Name=start;Parent=BBa_K200003
BBa_K318505	.	stop_codon	1851	1853	.	+	0	ID=annotation2018142;Name=stop;Parent=BBa_K200003
BBa_K318505	.	CDS	594	1850	.	+	0	ID=annotation2018140;Name=Waa Ligase;Parent=BBa_K200003
BBa_K318505	.	stop_codon	1854	1856	.	+	0	ID=annotation2027407;Name=stop;Parent=BBa_K200003
BBa_K318505	.	terminator	1953	1993	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K318505	.	engineered_region	1953	1993	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K318505	.	stop_codon	1986	1986	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K318505	.	stem_loop	1960	1979	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K318505	.	polyA_site	1980	1993	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K318505
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgacaaacctgacactggatgtaaacattatcgatttcccatcaatacctgtggcgatgttgccgcaccgctgtag
ccctgaattgctcaactacagcgtggcgaaatttatcatgtggcgtaaagagacggggctttctcctgttaaccaaagc
cagacttttggcgtcgcctgggacgaccctgccaccaccgcaccggaagcgtttcgctttgatatctgcggcagcgtta
gcgaaccgattcccgataatcgttatggtgtgagcaatggtgaacttaccggtggacgttatgccgtggcccgccacgt
tggcgagctggacgatatttcacacacggtatggggcatcattcgccactggctgcctgcaagcggcgagaaaatgcgt
aaagcaccgattctgtttcactacaccaatcttgccgaaggggtgacagagcagcgactggaaacggatgtttatgtgc
cgttggcgtgataatactagagaaagaggagaaatactagatgctaacatcctttaaacttcattcattgaaaccttac
actctgaaatcatcaatgattttagagataataacttatatattatgttttttttcaatgataattgcattcgtcgata
atactttcagcataaaaatatataatatcactgctatagtttgcttattgtcactaattttacgtggcagacaagaaaa
ttataatataaaaaaccttattcttcccctttctatatttttaataggcttgcttgatttaatttggtattctgcgttt
aaagtagataattcgccatttcgtgctacttaccatagttatttaaatactgccaaaatatttatatttggttctttta
ttgttttcttgacactaactagccagctaaaatcaaaaaaagagagtgtattatacactttgtattctctgtcatttct
aattgctggatatgcaatgtatattaatagcattcatgaaaatgaccgcatttcttttggtgtaggaacggcaacagga
gcagcatattcaacaatgctaatagggatagttagtggcgttgcgattctttatactaagaaaaatcatccttttttat
ttttattaaatagttgcgcggtactttatgttctggcgctaacacaaaccagagcaaccctactcctgttccctataat
ttgtgttgctgcattaatagcttattataataaatcacccaagaaattcacttcctctattgttctactaattgctata
ttagctagcattgttattatatttaataaaccaatacagaatcgctataatgaagcattaaatgacttaaacagttata
ccaatgctaatagtgttacttccctaggtgcaagactggcaatgtacgaaattggtttaaatatattcataaagtcacc
tttttcatttagatcagcagagtcacgcgctgaaagtatgaatttgttagttgcagaacacaataggctaagaggggca
ttggagttttctaacgtacatctacataatgagataattgaagcagggtcactgaaaggtctgatgggaattttttcca
cacttttcctctatttttcactattttatatagcatataaaaaacgagctttgggtttgttgatattaacgcttggcat
tgtggggattggactcagtgatgtgatcatatgggcacgcagcattccaattatcattatatccgctatagtcctctta
ctcgtcattaataatcgtaacaatacaattaattaataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttata