BBa_K318505
type: ComponentDefinition
persistent identity: BBa K318505
display id: BBa_K318505
version: 1
was generated by: iGEM to SBOL conversion
description: lacI pL + RBS + YgiV + RBS + RfaI + TT
created: 2010-07-17T11:00:00Z
modified: 2015-05-08T01:11:58Z
mutable provenance: uc
owned by: james
owned by: myers
mutable description: uc
discontinued: false
dominant: false
group u list: _438_
m user id: 0
owner id: 4504
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: uc
star: false
creator: Sarah R. Sandock
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0011
component: BBa_K200002
component: BBa_B0034
component: BBa_K200003
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_K200002
sequence annotation: BBa_K200003
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K318505_sequence/1

BBa_R0011
type: Component
persistent identity: component2074305
display id: component2074305
version: 1
top level: BBa_K318505
definition: lacI+pL
access: public

BBa_K200002
type: Component
persistent identity: component2074316
display id: component2074316
version: 1
top level: BBa_K318505
definition: ygiV
access: public

BBa_B0034
type: Component
persistent identity: component2074311
display id: component2074311
version: 1
top level: BBa_K318505
definition: BBa_B0034
access: public

BBa_K200003
type: Component
persistent identity: component2074323
display id: component2074323
version: 1
top level: BBa_K318505
definition: WaaL Ligas
access: public

BBa_B0034
type: Component
persistent identity: component2074318
display id: component2074318
version: 1
top level: BBa_K318505
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2074326
display id: component2074326
version: 1
top level: BBa_K318505
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2074324
display id: component2074324
version: 1
top level: BBa_K318505
definition: BBa_B0010
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2074305
display id: annotation2074305
version: 1
top level: BBa_K318505
location: https://synbiohub.org/public/igem/BBa_K318505/annotation2074305/range2074305/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K318505/annotation2074305/range2074305/1
type: Range
persistent identity: range2074305
display id: range2074305
version: 1
top level: BBa_K318505
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2074311
display id: annotation2074311
version: 1
top level: BBa_K318505
location: https://synbiohub.org/public/igem/BBa_K318505/annotation2074311/range2074311/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K318505/annotation2074311/range2074311/1
type: Range
persistent identity: range2074311
display id: range2074311
version: 1
top level: BBa_K318505
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_K200002
type: SequenceAnnotation
persistent identity: annotation2074316
display id: annotation2074316
version: 1
top level: BBa_K318505
location: https://synbiohub.org/public/igem/BBa_K318505/annotation2074316/range2074316/1
component: BBa_K200002

https://synbiohub.org/public/igem/BBa_K318505/annotation2074316/range2074316/1
type: Range
persistent identity: range2074316
display id: range2074316
version: 1
top level: BBa_K318505
direction: direction/reverse
start: 82
end: 567
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2074318
display id: annotation2074318
version: 1
top level: BBa_K318505
location: https://synbiohub.org/public/igem/BBa_K318505/annotation2074318/range2074318/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K318505/annotation2074318/range2074318/1
type: Range
persistent identity: range2074318
display id: range2074318
version: 1
top level: BBa_K318505
direction: direction/reverse
start: 576
end: 587
orientation: inline

BBa_K200003
type: SequenceAnnotation
persistent identity: annotation2074323
display id: annotation2074323
version: 1
top level: BBa_K318505
location: https://synbiohub.org/public/igem/BBa_K318505/annotation2074323/range2074323/1
component: BBa_K200003

https://synbiohub.org/public/igem/BBa_K318505/annotation2074323/range2074323/1
type: Range
persistent identity: range2074323
display id: range2074323
version: 1
top level: BBa_K318505
direction: direction/reverse
start: 594
end: 1856
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2074324
display id: annotation2074324
version: 1
top level: BBa_K318505
location: https://synbiohub.org/public/igem/BBa_K318505/annotation2074324/range2074324/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K318505/annotation2074324/range2074324/1
type: Range
persistent identity: range2074324
display id: range2074324
version: 1
top level: BBa_K318505
direction: direction/forward
start: 1865
end: 1944
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2074326
display id: annotation2074326
version: 1
top level: BBa_K318505
location: https://synbiohub.org/public/igem/BBa_K318505/annotation2074326/range2074326/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K318505/annotation2074326/range2074326/1
type: Range
persistent identity: range2074326
display id: range2074326
version: 1
top level: BBa_K318505
direction: direction/forward
start: 1953
end: 1993
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

ygiV
type: ComponentDefinition
persistent identity: BBa K200002
display id: BBa_K200002
version: 1
was generated by: iGEM to SBOL conversion
description: Colanic acid global regulator -> ygiV (B3023)
created: 2009-08-11T11:00:00Z
modified: 2015-05-08T01:11:21Z
mutable provenance: NCBI Reference Sequence: NC_000913.2 >gi|49175990:3166771-3167253 Escherichia coli str. K-12 substr. MG1655, complete genome
owned by: james
owned by: myers
mutable description: The putative transcription factor B3023 acts as a repressor for the yncC promoter. YncC increases biofilm formation by repressing overproduction of the exopolysaccharide identified as colanic acid. Therefore B3023 increases the production of colanic acid by blocking YncC.
discontinued: false
dominant: false
experience: experience/None
group u list: _298_
m user id: 0
owner id: 5277
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence of NCBI is the reverse compliment.
star: false
creator: James Field
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cds
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K200002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2018131
display id: annotation2018131
version: 1
top level: ygiV
location: https://synbiohub.org/public/igem/BBa_K200002/annotation2018131/range2018131/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K200002/annotation2018131/range2018131/1
type: Range
persistent identity: range2018131
display id: range2018131
version: 1
top level: ygiV
direction: direction/forward
start: 1
end: 480
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2018130
display id: annotation2018130
version: 1
top level: ygiV
location: https://synbiohub.org/public/igem/BBa_K200002/annotation2018130/range2018130/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K200002/annotation2018130/range2018130/1
type: Range
persistent identity: range2018130
display id: range2018130
version: 1
top level: ygiV
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2018132
display id: annotation2018132
version: 1
top level: ygiV
location: https://synbiohub.org/public/igem/BBa_K200002/annotation2018132/range2018132/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K200002/annotation2018132/range2018132/1
type: Range
persistent identity: range2018132
display id: range2018132
version: 1
top level: ygiV
direction: direction/forward
start: 481
end: 483
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2027405
display id: annotation2027405
version: 1
top level: ygiV
location: https://synbiohub.org/public/igem/BBa_K200002/annotation2027405/range2027405/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K200002/annotation2027405/range2027405/1
type: Range
persistent identity: range2027405
display id: range2027405
version: 1
top level: ygiV
direction: direction/forward
start: 484
end: 486
orientation: inline

WaaL Ligas
type: ComponentDefinition
persistent identity: BBa K200003
display id: BBa_K200003
version: 1
was generated by: iGEM to SBOL conversion
description: Rfal
created: 2009-08-11T11:00:00Z
modified: 2015-05-08T01:11:21Z
mutable provenance: NCBI Reference Sequence: NC_000913.2 >gi|49175990:3794971-3796230 Escherichia coli str. K-12 substr. MG1655, complete genome
owned by: james
owned by: myers
mutable description: Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the lipid A core, in K-12 it links colanic acid to the lipid A core. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.
discontinued: false
dominant: false
experience: experience/None
group u list: _298_
m user id: 0
owner id: 5277
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Charles Fracchia, James Field
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: Waa Ligase
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K200003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2018141
display id: annotation2018141
version: 1
top level: WaaL Ligas
location: https://synbiohub.org/public/igem/BBa_K200003/annotation2018141/range2018141/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K200003/annotation2018141/range2018141/1
type: Range
persistent identity: range2018141
display id: range2018141
version: 1
top level: WaaL Ligas
direction: direction/forward
start: 1
end: 3
orientation: inline

Waa Ligase
type: SequenceAnnotation
persistent identity: annotation2018140
display id: annotation2018140
version: 1
top level: WaaL Ligas
location: https://synbiohub.org/public/igem/BBa_K200003/annotation2018140/range2018140/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K200003/annotation2018140/range2018140/1
type: Range
persistent identity: range2018140
display id: range2018140
version: 1
top level: WaaL Ligas
direction: direction/forward
start: 1
end: 1257
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2018142
display id: annotation2018142
version: 1
top level: WaaL Ligas
location: https://synbiohub.org/public/igem/BBa_K200003/annotation2018142/range2018142/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K200003/annotation2018142/range2018142/1
type: Range
persistent identity: range2018142
display id: range2018142
version: 1
top level: WaaL Ligas
direction: direction/forward
start: 1258
end: 1260
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2027407
display id: annotation2027407
version: 1
top level: WaaL Ligas
location: https://synbiohub.org/public/igem/BBa_K200003/annotation2027407/range2027407/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K200003/annotation2027407/range2027407/1
type: Range
persistent identity: range2027407
display id: range2027407
version: 1
top level: WaaL Ligas
direction: direction/forward
start: 1261
end: 1263
orientation: inline

https://synbiohub.org/public/igem/BBa_K318505_sequence/1
type: Sequence
persistent identity: BBa K318505 sequence
display id: BBa_K318505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgacaaacctgacactggatgtaaacattatcgatttcccatcaatacctgtggcgatgttgccgcaccgctgtagccctgaattgctcaactacagcgtggcgaaatttatcatgtggcgtaaagagacggggctttctcctgttaaccaaagccagacttttggcgtcgcctgggacgaccctgccaccaccgcaccggaagcgtttcgctttgatatctgcggcagcgttagcgaaccgattcccgataatcgttatggtgtgagcaatggtgaacttaccggtggacgttatgccgtggcccgccacgttggcgagctggacgatatttcacacacggtatggggcatcattcgccactggctgcctgcaagcggcgagaaaatgcgtaaagcaccgattctgtttcactacaccaatcttgccgaaggggtgacagagcagcgactggaaacggatgtttatgtgccgttggcgtgataatactagagaaagaggagaaatactagatgctaacatcctttaaacttcattcattgaaaccttacactctgaaatcatcaatgattttagagataataacttatatattatgttttttttcaatgataattgcattcgtcgataatactttcagcataaaaatatataatatcactgctatagtttgcttattgtcactaattttacgtggcagacaagaaaattataatataaaaaaccttattcttcccctttctatatttttaataggcttgcttgatttaatttggtattctgcgtttaaagtagataattcgccatttcgtgctacttaccatagttatttaaatactgccaaaatatttatatttggttcttttattgttttcttgacactaactagccagctaaaatcaaaaaaagagagtgtattatacactttgtattctctgtcatttctaattgctggatatgcaatgtatattaatagcattcatgaaaatgaccgcatttcttttggtgtaggaacggcaacaggagcagcatattcaacaatgctaatagggatagttagtggcgttgcgattctttatactaagaaaaatcatccttttttatttttattaaatagttgcgcggtactttatgttctggcgctaacacaaaccagagcaaccctactcctgttccctataatttgtgttgctgcattaatagcttattataataaatcacccaagaaattcacttcctctattgttctactaattgctatattagctagcattgttattatatttaataaaccaatacagaatcgctataatgaagcattaaatgacttaaacagttataccaatgctaatagtgttacttccctaggtgcaagactggcaatgtacgaaattggtttaaatatattcataaagtcacctttttcatttagatcagcagagtcacgcgctgaaagtatgaatttgttagttgcagaacacaataggctaagaggggcattggagttttctaacgtacatctacataatgagataattgaagcagggtcactgaaaggtctgatgggaattttttccacacttttcctctatttttcactattttatatagcatataaaaaacgagctttgggtttgttgatattaacgcttggcattgtggggattggactcagtgatgtgatcatatgggcacgcagcattccaattatcattatatccgctatagtcctcttactcgtcattaataatcgtaacaatacaattaattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K200002_sequence/1
type: Sequence
persistent identity: BBa K200002 sequence
display id: BBa_K200002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacaaacctgacactggatgtaaacattatcgatttcccatcaatacctgtggcgatgttgccgcaccgctgtagccctgaattgctcaactacagcgtggcgaaatttatcatgtggcgtaaagagacggggctttctcctgttaaccaaagccagacttttggcgtcgcctgggacgaccctgccaccaccgcaccggaagcgtttcgctttgatatctgcggcagcgttagcgaaccgattcccgataatcgttatggtgtgagcaatggtgaacttaccggtggacgttatgccgtggcccgccacgttggcgagctggacgatatttcacacacggtatggggcatcattcgccactggctgcctgcaagcggcgagaaaatgcgtaaagcaccgattctgtttcactacaccaatcttgccgaaggggtgacagagcagcgactggaaacggatgtttatgtgccgttggcgtgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K200003_sequence/1
type: Sequence
persistent identity: BBa K200003 sequence
display id: BBa_K200003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctaacatcctttaaacttcattcattgaaaccttacactctgaaatcatcaatgattttagagataataacttatatattatgttttttttcaatgataattgcattcgtcgataatactttcagcataaaaatatataatatcactgctatagtttgcttattgtcactaattttacgtggcagacaagaaaattataatataaaaaaccttattcttcccctttctatatttttaataggcttgcttgatttaatttggtattctgcgtttaaagtagataattcgccatttcgtgctacttaccatagttatttaaatactgccaaaatatttatatttggttcttttattgttttcttgacactaactagccagctaaaatcaaaaaaagagagtgtattatacactttgtattctctgtcatttctaattgctggatatgcaatgtatattaatagcattcatgaaaatgaccgcatttcttttggtgtaggaacggcaacaggagcagcatattcaacaatgctaatagggatagttagtggcgttgcgattctttatactaagaaaaatcatccttttttatttttattaaatagttgcgcggtactttatgttctggcgctaacacaaaccagagcaaccctactcctgttccctataatttgtgttgctgcattaatagcttattataataaatcacccaagaaattcacttcctctattgttctactaattgctatattagctagcattgttattatatttaataaaccaatacagaatcgctataatgaagcattaaatgacttaaacagttataccaatgctaatagtgttacttccctaggtgcaagactggcaatgtacgaaattggtttaaatatattcataaagtcacctttttcatttagatcagcagagtcacgcgctgaaagtatgaatttgttagttgcagaacacaataggctaagaggggcattggagttttctaacgtacatctacataatgagataattgaagcagggtcactgaaaggtctgatgggaattttttccacacttttcctctatttttcactattttatatagcatataaaaaacgagctttgggtttgttgatattaacgcttggcattgtggggattggactcagtgatgtgatcatatgggcacgcagcattccaattatcattatatccgctatagtcctcttactcgtcattaataatcgtaacaatacaattaattaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z