LOCUS       BBa_K318507             1910 bp    DNA     linear       18-May-2024
DEFINITION  Pir + RBS + Lysis Cassette
ACCESSION   BBa_K318507
VERSION     BBa_K318507.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             142..798
                     /label=T4 holin
     CDS             126..801
                     /label=BBa_K112805
     CDS             826..1320
                     /label=T4 Endolysin
     CDS             810..1323
                     /label=BBa_K112806
     stem_loop       1343..1386
                     /label=stem_loop
     misc_feature    1332..1411
                     /label=BBa_B0010
     terminator      1332..1411
                     /label=BBa_B0010
     stem_loop       1427..1446
                     /label=T7 TE
     misc_feature    1453..1453
                     /label=stop
     polyA_site      1447..1460
                     /label=polya
     misc_feature    1420..1460
                     /label=BBa_B0012
     terminator      1420..1460
                     /label=BBa_B0012
     promoter        1469..1503
                     /label=BBa_J23116
     CDS             1526..1819
                     /label=T4 antiholin
     CDS             1512..1822
                     /label=BBa_K112807
     stem_loop       1842..1885
                     /label=stem_loop
     misc_feature    1831..1910
                     /label=BBa_B0010
     terminator      1831..1910
                     /label=BBa_B0010
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagagcttaa aaggagggtc tatggcagca cctagaatat cattttcgcc ctctgatatt
      181 ctatttggtg ttctcgatcg cttgttcaaa gataacgcta ccgggaaggt tcttgcttcc
      241 cgggtagctg tcgtaattct tttgtttata atggcgattg tttggtatag gggagatagt
      301 ttctttgagt actataagca atcaaagtat gaaacataca gtgaaattat tgaaaaggaa
      361 agaactgcac gctttgaatc tgtcgccctg gaacaactcc agatagttca tatatcatct
      421 gaggcagact ttagtgcggt gtattctttc cgccctaaaa acttaaacta ttttgttgat
      481 attatagcat acgaaggaaa attaccttca acaataagtg aaaaatcact tggaggatat
      541 cctgttgata aaactatgga tgaatataca gttcatttaa atggacgtca ttattattcc
      601 aactcaaaat ttgctttttt accaactaaa aagcctactc ccgaaataaa ctacatgtac
      661 agttgtccat attttaattt ggataatatc tatgctggaa cgataaccat gtactggtat
      721 agaaatgatc atataagtaa tgaccgcctt gaatcaatat gtgctcaggc ggccagaata
      781 ttaggaaggg ctaaataatt atactagaga tacttaggag gtattatgaa tatatttgaa
      841 atgttacgta tagatgaagg tcttagactt aaaatctata aagacacaga aggctattac
      901 actattggca tcggtcattt gcttacaaaa agtccatcac ttaatgctgc taaatctgaa
      961 ttagataaag ctattgggcg taattgcaat ggtgtaatta caaaagatga ggctgaaaaa
     1021 ctctttaatc aggatgttga tgctgctgtt cgcggaatcc tgagaaatgc taaattaaaa
     1081 ccggtttatg attctcttga tgcggttcgt cgctgtgcat tgattaatat ggttttccaa
     1141 atgggagaaa ccggtgtggc aggatttact aactctttac gtatgcttca acaaaaacgc
     1201 tgggatgaag cagcagttaa cttagctaaa agtagatggt ataatcaaac acctaatcgc
     1261 gcaaaacgag tcattacaac gtttagaact ggcacttggg acgcgtataa aaatctataa
     1321 agctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1381 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1441 tgggcctttc tgcgtttata tactagagtt gacagctagc tcagtcctag ggactatgct
     1501 agctactaga ggataggagg cctttatggc cttaaaagca acagcacttt ttgccatgct
     1561 aggattgtca tttgttttat ctccatcgat tgaagcgaat gtcgatcctc attttgataa
     1621 atttatggaa tctggtatta ggcacgttta tatgcttttt gaaaataaaa gcgtagaatc
     1681 gtctgaacaa ttctatagtt ttatgagaac gacctataaa aatgacccgt gctcttctga
     1741 ttttgaatgt atagagcgag gcgcggagat ggcacaatca tacgctagaa ttatgaacat
     1801 taaattggag actgaatgaa attactagag ccaggcatca aataaaacga aaggctcagt
     1861 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
//