LOCUS       BBa_K318510              960 bp    DNA     linear       16-Dec-2024
DEFINITION  lacI pL + RBS + cI + TT
ACCESSION   BBa_K318510
VERSION     BBa_K318510.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    1..75
                     /label=BBa_K200021
     CDS             82..798
                     /label=cI lambda
     misc_feature    799..823
                     /label=Help:Barcodes
     CDS             82..823
                     /label=BBa_I9200
     stem_loop       843..886
                     /label=stem_loop
     misc_feature    832..911
                     /label=BBa_B0010
     terminator      832..911
                     /label=BBa_B0010
     stem_loop       927..946
                     /label=T7 TE
     misc_feature    953..953
                     /label=stop
     polyA_site      947..960
                     /label=polya
     misc_feature    920..960
                     /label=BBa_B0012
     terminator      920..960
                     /label=BBa_B0012
     terminator      832..960
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagcact aaaaagaaac cacttactca agagcagtta
      121 gaggacgccc gtcgcttaaa agccatttat gaaaaaaaga aaaatgaatt aggcctttcc
      181 caggaatcag tcgccgacaa gatggggatg gggcagtcgg gcgttggtgc tctttttaat
      241 ggcatcaatg cccttaatgc ttataacgcg gccctcttag ccaaaatttt gaaagttagc
      301 gttgaagaat ttagcccttc gatcgcgcga gaaatctacg agatgtatga agcagttagt
      361 atgcagccgt cgttacgaag tgagtatgag taccctgttt tttcacatgt tcaggccggg
      421 atgttctcgc ctgagttacg aacctttacc aaaggtgatg cagagcgatg ggtaagcact
      481 accaaaaaag cgagtgattc agccttctgg ttagaggttg aaggtaattc catgaccgcc
      541 ccaactggct ccaagccaag ctttcctgac ggaatgctta ttttggttga ccctgagcag
      601 gctgttgagc caggtgattt ctgcatagcg cgattagggg gtgatgagtt taccttcaag
      661 aaactgatca gggatagcgg tcaggtgttt cttcaaccac taaacccaca gtacccaatg
      721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa
      781 gagacgtttg gctaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc
      841 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      901 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//