LOCUS       BBa_K318513             1113 bp    DNA     linear       22-Nov-2024
DEFINITION  gadA/RBS + RFP + TT
ACCESSION   BBa_K318513
VERSION     BBa_K318513.1  
FEATURES             Location/Qualifiers
     promoter        1..264
                     /label=BBa_K318512
     CDS             271..945
                     /label=mrfp1
     misc_feature    952..976
                     /label=Help:Barcodes
     CDS             271..951
                     /label=BBa_E1010
     stem_loop       996..1039
                     /label=stem_loop
     misc_feature    985..1064
                     /label=BBa_B0010
     terminator      985..1064
                     /label=BBa_B0010
     stem_loop       1080..1099
                     /label=T7 TE
     misc_feature    1106..1106
                     /label=stop
     polyA_site      1100..1113
                     /label=polya
     misc_feature    1073..1113
                     /label=BBa_B0012
     terminator      1073..1113
                     /label=BBa_B0012
     misc_feature    271..1113
                     /label=BBa_I13501
ORIGIN
        1 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
       61 tttgatcgcc cgaacagcaa tgtttgggcg atttttatta cgataataaa gtctgttttt
      121 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
      181 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
      241 cttcaaataa atttaaggag ttcgtactag atggcttcct ccgaagacgt tatcaaagag
      301 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
      361 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
      421 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
      481 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
      541 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
      601 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
      661 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
      721 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
      781 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
      841 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
      901 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
      961 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1021 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1081 gctcaccttc gggtgggcct ttctgcgttt ata
//