LOCUS       BBa_K318515             1684 bp    DNA     linear       23-Dec-2024
DEFINITION  acrRA + RFP + TT
ACCESSION   BBa_K318515
VERSION     BBa_K318515.1  
FEATURES             Location/Qualifiers
     promoter        1..835
                     /label=BBa_K318514
     CDS             842..1516
                     /label=mrfp1
     misc_feature    1523..1547
                     /label=Help:Barcodes
     CDS             842..1522
                     /label=BBa_E1010
     stem_loop       1567..1610
                     /label=stem_loop
     misc_feature    1556..1635
                     /label=BBa_B0010
     terminator      1556..1635
                     /label=BBa_B0010
     stem_loop       1651..1670
                     /label=T7 TE
     misc_feature    1677..1677
                     /label=stop
     polyA_site      1671..1684
                     /label=polya
     misc_feature    1644..1684
                     /label=BBa_B0012
     terminator      1644..1684
                     /label=BBa_B0012
     misc_feature    842..1684
                     /label=BBa_I13501
ORIGIN
        1 ttatcagggg gagccgttga ccgtcgacgc gcgcagcgtc ggacacaatt gatacatctc
       61 cagcaggatc gtgacgtagg cgcgagcttc tttttttaaa tcaaacgatt gcggagcaaa
      121 taaccagttt tccatgagcc ctgaaataaa gctgcgcatc agtatcgccg cacgacgggt
      181 cagcagattt tcaggcagca ttttagcatt aatacaatgt tttaacgttt gttcaatccg
      241 atcgtaactt tccagacaaa ggctacgttg cgcctgttga accacaacca tttctccgac
      301 aaactcacat ttatggaata taatctccat caataagcgg cgtcgttctt ctgttacagt
      361 agcttcaaga atatgaacta aaatttctct taatacagat agtggatcgt cggggaattt
      421 tgcctgatac tcaatctcaa gctcaccaat attggattct gatagctccc agatctcact
      481 gaataaatcc gacttatttt tgaaatgcca atagattgcg ccgcgcgtca cgccagcagc
      541 gttcgcaatc tccgccagcg aggttgccga tacgccttgc tgcgagaaca aacgcagggc
      601 cacatccagg atgtgttgtc gtgtctccag cgcttgttgt ttggtttttc gtgccatatg
      661 tcggtgaatt tacaggcgtt agatttacat acatttatgg atgtatgtac catagcacga
      721 cgataatata aacgcagcaa tgggtttaag gacctttgac cattgaccaa tttgaaatcg
      781 gacactcgag gtttacatat gaacaaaaac agagggttaa cgcctctggc ggtcgtacta
      841 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
      901 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
      961 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     1021 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     1081 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
     1141 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
     1201 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
     1261 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
     1321 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     1381 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
     1441 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
     1501 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
     1561 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1621 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1681 tata
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