LOCUS BBa_K318519 3163 bp DNA linear 22-Nov-2024 DEFINITION Bile Salt Induced Lysis Cassette ACCESSION BBa_K318519 VERSION BBa_K318519.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 CDS 44..390 /label=BBa_K318516 stem_loop 410..453 /label=stem_loop misc_feature 399..478 /label=BBa_B0010 terminator 399..478 /label=BBa_B0010 stem_loop 494..513 /label=T7 TE misc_feature 520..520 /label=stop polyA_site 514..527 /label=polya misc_feature 487..527 /label=BBa_B0012 terminator 487..527 /label=BBa_B0012 promoter 536..1370 /label=BBa_K318514 CDS 1395..2051 /label=T4 holin CDS 1379..2054 /label=BBa_K112805 CDS 2079..2573 /label=T4 Endolysin CDS 2063..2576 /label=BBa_K112806 stem_loop 2596..2639 /label=stem_loop misc_feature 2585..2664 /label=BBa_B0010 terminator 2585..2664 /label=BBa_B0010 stem_loop 2680..2699 /label=T7 TE misc_feature 2706..2706 /label=stop polyA_site 2700..2713 /label=polya misc_feature 2673..2713 /label=BBa_B0012 terminator 2673..2713 /label=BBa_B0012 promoter 2722..2756 /label=BBa_J23116 CDS 2779..3072 /label=T4 antiholin CDS 2765..3075 /label=BBa_K112807 stem_loop 3095..3138 /label=stem_loop misc_feature 3084..3163 /label=BBa_B0010 terminator 3084..3163 /label=BBa_B0010 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gaggatgacc atttccgctc 61 aggttatcga cacgattgtc gagtggattg atgataattt gaatcagccg ttacgtattg 121 atgatattgc ccgtcatgcg gggtattcca agtggcacct gcagcgcctt tttatgcagt 181 acaaagggga gagtctggga cgctacgtgc gtgaacggaa gctaaaactg gcggcgcgcg 241 acctgctcga caccgaccag aaggtgtatg atatttgtct caagtatggt tttgattcgc 301 agcaaacctt tacgcgcatt tttacccgca cgttcaacct gccgccaggc gcttatcgta 361 aagaaaagca tggccgtacg cattgaaatg tactagagcc aggcatcaaa taaaacgaaa 421 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 481 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagttatc 541 agggggagcc gttgaccgtc gacgcgcgca gcgtcggaca caattgatac atctccagca 601 ggatcgtgac gtaggcgcga gcttcttttt ttaaatcaaa cgattgcgga gcaaataacc 661 agttttccat gagccctgaa ataaagctgc gcatcagtat cgccgcacga cgggtcagca 721 gattttcagg cagcatttta gcattaatac aatgttttaa cgtttgttca atccgatcgt 781 aactttccag acaaaggcta cgttgcgcct gttgaaccac aaccatttct ccgacaaact 841 cacatttatg gaatataatc tccatcaata agcggcgtcg ttcttctgtt acagtagctt 901 caagaatatg aactaaaatt tctcttaata cagatagtgg atcgtcgggg aattttgcct 961 gatactcaat ctcaagctca ccaatattgg attctgatag ctcccagatc tcactgaata 1021 aatccgactt atttttgaaa tgccaataga ttgcgccgcg cgtcacgcca gcagcgttcg 1081 caatctccgc cagcgaggtt gccgatacgc cttgctgcga gaacaaacgc agggccacat 1141 ccaggatgtg ttgtcgtgtc tccagcgctt gttgtttggt ttttcgtgcc atatgtcggt 1201 gaatttacag gcgttagatt tacatacatt tatggatgta tgtaccatag cacgacgata 1261 atataaacgc agcaatgggt ttaaggacct ttgaccattg accaatttga aatcggacac 1321 tcgaggttta catatgaaca aaaacagagg gttaacgcct ctggcggtcg tactagagct 1381 taaaaggagg gtctatggca gcacctagaa tatcattttc gccctctgat attctatttg 1441 gtgttctcga tcgcttgttc aaagataacg ctaccgggaa ggttcttgct tcccgggtag 1501 ctgtcgtaat tcttttgttt ataatggcga ttgtttggta taggggagat agtttctttg 1561 agtactataa gcaatcaaag tatgaaacat acagtgaaat tattgaaaag gaaagaactg 1621 cacgctttga atctgtcgcc ctggaacaac tccagatagt tcatatatca tctgaggcag 1681 actttagtgc ggtgtattct ttccgcccta aaaacttaaa ctattttgtt gatattatag 1741 catacgaagg aaaattacct tcaacaataa gtgaaaaatc acttggagga tatcctgttg 1801 ataaaactat ggatgaatat acagttcatt taaatggacg tcattattat tccaactcaa 1861 aatttgcttt tttaccaact aaaaagccta ctcccgaaat aaactacatg tacagttgtc 1921 catattttaa tttggataat atctatgctg gaacgataac catgtactgg tatagaaatg 1981 atcatataag taatgaccgc cttgaatcaa tatgtgctca ggcggccaga atattaggaa 2041 gggctaaata attatactag agatacttag gaggtattat gaatatattt gaaatgttac 2101 gtatagatga aggtcttaga cttaaaatct ataaagacac agaaggctat tacactattg 2161 gcatcggtca tttgcttaca aaaagtccat cacttaatgc tgctaaatct gaattagata 2221 aagctattgg gcgtaattgc aatggtgtaa ttacaaaaga tgaggctgaa aaactcttta 2281 atcaggatgt tgatgctgct gttcgcggaa tcctgagaaa tgctaaatta aaaccggttt 2341 atgattctct tgatgcggtt cgtcgctgtg cattgattaa tatggttttc caaatgggag 2401 aaaccggtgt ggcaggattt actaactctt tacgtatgct tcaacaaaaa cgctgggatg 2461 aagcagcagt taacttagct aaaagtagat ggtataatca aacacctaat cgcgcaaaac 2521 gagtcattac aacgtttaga actggcactt gggacgcgta taaaaatcta taaagctact 2581 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2641 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2701 ttctgcgttt atatactaga gttgacagct agctcagtcc tagggactat gctagctact 2761 agaggatagg aggcctttat ggccttaaaa gcaacagcac tttttgccat gctaggattg 2821 tcatttgttt tatctccatc gattgaagcg aatgtcgatc ctcattttga taaatttatg 2881 gaatctggta ttaggcacgt ttatatgctt tttgaaaata aaagcgtaga atcgtctgaa 2941 caattctata gttttatgag aacgacctat aaaaatgacc cgtgctcttc tgattttgaa 3001 tgtatagagc gaggcgcgga gatggcacaa tcatacgcta gaattatgaa cattaaattg 3061 gagactgaat gaaattacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3121 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctc // LOCUS BBa_K318518 3163 bp DNA linear 22-Nov-2024 DEFINITION Bile Salt Induced Lysis Cassette ACCESSION BBa_K318518 VERSION BBa_K318518.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 CDS 44..390 /label=BBa_K318516 stem_loop 410..453 /label=stem_loop misc_feature 399..478 /label=BBa_B0010 terminator 399..478 /label=BBa_B0010 stem_loop 494..513 /label=T7 TE misc_feature 520..520 /label=stop polyA_site 514..527 /label=polya misc_feature 487..527 /label=BBa_B0012 terminator 487..527 /label=BBa_B0012 promoter 536..1370 /label=BBa_K318514 CDS 1395..2051 /label=T4 holin CDS 1379..2054 /label=BBa_K112805 CDS 2079..2573 /label=T4 Endolysin CDS 2063..2576 /label=BBa_K112806 stem_loop 2596..2639 /label=stem_loop misc_feature 2585..2664 /label=BBa_B0010 terminator 2585..2664 /label=BBa_B0010 stem_loop 2680..2699 /label=T7 TE misc_feature 2706..2706 /label=stop polyA_site 2700..2713 /label=polya misc_feature 2673..2713 /label=BBa_B0012 terminator 2673..2713 /label=BBa_B0012 promoter 2722..2756 /label=BBa_J23116 CDS 2779..3072 /label=T4 antiholin CDS 2765..3075 /label=BBa_K112807 stem_loop 3095..3138 /label=stem_loop misc_feature 3084..3163 /label=BBa_B0010 terminator 3084..3163 /label=BBa_B0010 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gaggatgacc atttccgctc 61 aggttatcga cacgattgtc gagtggattg atgataattt gaatcagccg ttacgtattg 121 atgatattgc ccgtcatgcg gggtattcca agtggcacct gcagcgcctt tttatgcagt 181 acaaagggga gagtctggga cgctacgtgc gtgaacggaa gctaaaactg gcggcgcgcg 241 acctgctcga caccgaccag aaggtgtatg atatttgtct caagtatggt tttgattcgc 301 agcaaacctt tacgcgcatt tttacccgca cgttcaacct gccgccaggc gcttatcgta 361 aagaaaagca tggccgtacg cattgaaatg tactagagcc aggcatcaaa taaaacgaaa 421 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 481 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagttatc 541 agggggagcc gttgaccgtc gacgcgcgca gcgtcggaca caattgatac atctccagca 601 ggatcgtgac gtaggcgcga gcttcttttt ttaaatcaaa cgattgcgga gcaaataacc 661 agttttccat gagccctgaa ataaagctgc gcatcagtat cgccgcacga cgggtcagca 721 gattttcagg cagcatttta gcattaatac aatgttttaa cgtttgttca atccgatcgt 781 aactttccag acaaaggcta cgttgcgcct gttgaaccac aaccatttct ccgacaaact 841 cacatttatg gaatataatc tccatcaata agcggcgtcg ttcttctgtt acagtagctt 901 caagaatatg aactaaaatt tctcttaata cagatagtgg atcgtcgggg aattttgcct 961 gatactcaat ctcaagctca ccaatattgg attctgatag ctcccagatc tcactgaata 1021 aatccgactt atttttgaaa tgccaataga ttgcgccgcg cgtcacgcca gcagcgttcg 1081 caatctccgc cagcgaggtt gccgatacgc cttgctgcga gaacaaacgc agggccacat 1141 ccaggatgtg ttgtcgtgtc tccagcgctt gttgtttggt ttttcgtgcc atatgtcggt 1201 gaatttacag gcgttagatt tacatacatt tatggatgta tgtaccatag cacgacgata 1261 atataaacgc agcaatgggt ttaaggacct ttgaccattg accaatttga aatcggacac 1321 tcgaggttta catatgaaca aaaacagagg gttaacgcct ctggcggtcg tactagagct 1381 taaaaggagg gtctatggca gcacctagaa tatcattttc gccctctgat attctatttg 1441 gtgttctcga tcgcttgttc aaagataacg ctaccgggaa ggttcttgct tcccgggtag 1501 ctgtcgtaat tcttttgttt ataatggcga ttgtttggta taggggagat agtttctttg 1561 agtactataa gcaatcaaag tatgaaacat acagtgaaat tattgaaaag gaaagaactg 1621 cacgctttga atctgtcgcc ctggaacaac tccagatagt tcatatatca tctgaggcag 1681 actttagtgc ggtgtattct ttccgcccta aaaacttaaa ctattttgtt gatattatag 1741 catacgaagg aaaattacct tcaacaataa gtgaaaaatc acttggagga tatcctgttg 1801 ataaaactat ggatgaatat acagttcatt taaatggacg tcattattat tccaactcaa 1861 aatttgcttt tttaccaact aaaaagccta ctcccgaaat aaactacatg tacagttgtc 1921 catattttaa tttggataat atctatgctg gaacgataac catgtactgg tatagaaatg 1981 atcatataag taatgaccgc cttgaatcaa tatgtgctca ggcggccaga atattaggaa 2041 gggctaaata attatactag agatacttag gaggtattat gaatatattt gaaatgttac 2101 gtatagatga aggtcttaga cttaaaatct ataaagacac agaaggctat tacactattg 2161 gcatcggtca tttgcttaca aaaagtccat cacttaatgc tgctaaatct gaattagata 2221 aagctattgg gcgtaattgc aatggtgtaa ttacaaaaga tgaggctgaa aaactcttta 2281 atcaggatgt tgatgctgct gttcgcggaa tcctgagaaa tgctaaatta aaaccggttt 2341 atgattctct tgatgcggtt cgtcgctgtg cattgattaa tatggttttc caaatgggag 2401 aaaccggtgt ggcaggattt actaactctt tacgtatgct tcaacaaaaa cgctgggatg 2461 aagcagcagt taacttagct aaaagtagat ggtataatca aacacctaat cgcgcaaaac 2521 gagtcattac aacgtttaga actggcactt gggacgcgta taaaaatcta taaagctact 2581 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2641 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2701 ttctgcgttt atatactaga gttgacagct agctcagtcc tagggactat gctagctact 2761 agaggatagg aggcctttat ggccttaaaa gcaacagcac tttttgccat gctaggattg 2821 tcatttgttt tatctccatc gattgaagcg aatgtcgatc ctcattttga taaatttatg 2881 gaatctggta ttaggcacgt ttatatgctt tttgaaaata aaagcgtaga atcgtctgaa 2941 caattctata gttttatgag aacgacctat aaaaatgacc cgtgctcttc tgattttgaa 3001 tgtatagagc gaggcgcgga gatggcacaa tcatacgcta gaattatgaa cattaaattg 3061 gagactgaat gaaattacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3121 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctc //