BBa_K318518
1
BBa_K318518
Bile Salt Induced Lysis Cassette
2010-10-14T11:00:00Z
2015-05-08T01:11:58Z
uc
uc
true
false
_438_
0
4504
9
Discontinued
false
uc
false
Sarah R. Sandock
component2086608
1
BBa_K112805
component2086600
1
BBa_B0010
component2086613
1
BBa_B0012
component2086620
1
BBa_B0010
component2086610
1
BBa_K112806
component2086599
1
BBa_K318516
component2086606
1
BBa_K318514
component2086611
1
BBa_B0010
component2086598
1
BBa_J23100
component2086617
1
BBa_J23116
component2086619
1
BBa_K112807
component2086602
1
BBa_B0012
annotation2086602
1
BBa_B0012
range2086602
1
487
527
annotation2086620
1
BBa_B0010
range2086620
1
3084
3163
annotation2086598
1
BBa_J23100
range2086598
1
1
35
annotation2086600
1
BBa_B0010
range2086600
1
399
478
annotation2086611
1
BBa_B0010
range2086611
1
2585
2664
annotation2086617
1
BBa_J23116
range2086617
1
2722
2756
annotation2086606
1
BBa_K318514
range2086606
1
536
1370
annotation2086599
1
BBa_K318516
range2086599
1
44
390
annotation2086608
1
BBa_K112805
range2086608
1
1379
2054
annotation2086610
1
BBa_K112806
range2086610
1
2063
2576
annotation2086619
1
BBa_K112807
range2086619
1
2765
3075
annotation2086613
1
BBa_B0012
range2086613
1
2673
2713
BBa_K112806
1
BBa_K112806
[T4 endolysin]
2008-10-31T12:00:00Z
2015-05-08T01:09:19Z
Enterobacteria phage T4
http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
Released HQ 2013
The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
false
true
_224_
0
3025
171
In stock
false
High expression of lysozyme only is toxic to bacteria.
false
Jin Huh
annotation1999018
1
T4 Endolysin
range1999018
1
17
511
BBa_K112807
1
BBa_K112807
[T4 antiholin]
2008-10-31T12:00:00Z
2015-05-08T01:09:19Z
Enterobacteria phage T4
http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=59202&to=59495&view=gbwithparts
Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria.
false
true
_224_
0
3025
171
It's complicated
false
Higher constitutive expression of antiholin compared to the expression of holin is required for the stable lysis device.
false
Jin Huh
annotation1999019
1
T4 antiholin
range1999019
1
15
308
BBa_K318516
1
BBa_K318516
RamA
2010-09-25T11:00:00Z
2015-05-08T01:11:58Z
uc
uc
false
false
_438_
0
4504
9
Not in stock
false
uc
false
Sarah R. Sandock
BBa_K112805
1
BBa_K112805
[T4 holin]
2008-10-30T12:00:00Z
2015-05-08T01:09:19Z
Enterobacteria phage T4
http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts
Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.
false
true
_224_
0
3025
171
It's complicated
false
Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin.
false
Jin Huh
annotation1999005
1
T4 holin
range1999005
1
17
673
BBa_K318514
1
BBa_K318514
acrRA
2010-09-25T11:00:00Z
2015-05-08T01:11:58Z
uc
uc
false
false
_438_
0
4504
9
Not in stock
false
uc
false
Sarah R. Sandock
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J23116
1
BBa_J23116
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
false
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K318519
1
BBa_K318519
Bile Salt Induced Lysis Cassette
2010-10-14T11:00:00Z
2015-05-08T01:11:58Z
uc
uc
false
true
_438_
0
4504
9
Not in stock
true
uc
false
Sarah R. Sandock
component2086622
1
BBa_J23100
component2086624
1
BBa_B0010
component2086623
1
BBa_K318516
component2086630
1
BBa_K318514
component2086626
1
BBa_B0012
component2086641
1
BBa_J23116
component2086637
1
BBa_B0012
component2086644
1
BBa_B0010
component2086643
1
BBa_K112807
component2086635
1
BBa_B0010
component2086632
1
BBa_K112805
component2086634
1
BBa_K112806
annotation2086622
1
BBa_J23100
range2086622
1
1
35
annotation2086634
1
BBa_K112806
range2086634
1
2063
2576
annotation2086632
1
BBa_K112805
range2086632
1
1379
2054
annotation2086644
1
BBa_B0010
range2086644
1
3084
3163
annotation2086630
1
BBa_K318514
range2086630
1
536
1370
annotation2086635
1
BBa_B0010
range2086635
1
2585
2664
annotation2086623
1
BBa_K318516
range2086623
1
44
390
annotation2086626
1
BBa_B0012
range2086626
1
487
527
annotation2086641
1
BBa_J23116
range2086641
1
2722
2756
annotation2086643
1
BBa_K112807
range2086643
1
2765
3075
annotation2086624
1
BBa_B0010
range2086624
1
399
478
annotation2086637
1
BBa_B0012
range2086637
1
2673
2713
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_J23116_sequence
1
ttgacagctagctcagtcctagggactatgctagc
BBa_K318518_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagaggatgaccatttccgctcaggttatcgacacgattgtcgagtggattgatgataatttgaatcagccgttacgtattgatgatattgcccgtcatgcggggtattccaagtggcacctgcagcgcctttttatgcagtacaaaggggagagtctgggacgctacgtgcgtgaacggaagctaaaactggcggcgcgcgacctgctcgacaccgaccagaaggtgtatgatatttgtctcaagtatggttttgattcgcagcaaacctttacgcgcatttttacccgcacgttcaacctgccgccaggcgcttatcgtaaagaaaagcatggccgtacgcattgaaatgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatcagggggagccgttgaccgtcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatcaaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtcagcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaaggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcggcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaattttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttatttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgccttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgccatatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacgcagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacagagggttaacgcctctggcggtcgtactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K318514_sequence
1
ttatcagggggagccgttgaccgtcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatcaaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtcagcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaaggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcggcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaattttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttatttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgccttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgccatatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacgcagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacagagggttaacgcctctggcggtcg
BBa_K318516_sequence
1
gatgaccatttccgctcaggttatcgacacgattgtcgagtggattgatgataatttgaatcagccgttacgtattgatgatattgcccgtcatgcggggtattccaagtggcacctgcagcgcctttttatgcagtacaaaggggagagtctgggacgctacgtgcgtgaacggaagctaaaactggcggcgcgcgacctgctcgacaccgaccagaaggtgtatgatatttgtctcaagtatggttttgattcgcagcaaacctttacgcgcatttttacccgcacgttcaacctgccgccaggcgcttatcgtaaagaaaagcatggccgtacgcattgaaatg
BBa_K112805_sequence
1
cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta
BBa_K318519_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagaggatgaccatttccgctcaggttatcgacacgattgtcgagtggattgatgataatttgaatcagccgttacgtattgatgatattgcccgtcatgcggggtattccaagtggcacctgcagcgcctttttatgcagtacaaaggggagagtctgggacgctacgtgcgtgaacggaagctaaaactggcggcgcgcgacctgctcgacaccgaccagaaggtgtatgatatttgtctcaagtatggttttgattcgcagcaaacctttacgcgcatttttacccgcacgttcaacctgccgccaggcgcttatcgtaaagaaaagcatggccgtacgcattgaaatgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatcagggggagccgttgaccgtcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatcaaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtcagcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaaggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcggcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaattttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttatttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgccttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgccatatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacgcagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacagagggttaacgcctctggcggtcgtactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K112807_sequence
1
gataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaat
BBa_K112806_sequence
1
atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z