BBa_K318519
type: ComponentDefinition
persistent identity: BBa K318519
display id: BBa_K318519
version: 1
was generated by: iGEM to SBOL conversion
description: Bile Salt Induced Lysis Cassette
created: 2010-10-14T11:00:00Z
modified: 2015-05-08T01:11:58Z
mutable provenance: uc
owned by: james
owned by: myers
mutable description: uc
discontinued: false
dominant: true
experience: experience/None
group u list: _438_
m user id: 0
owner id: 4504
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: uc
star: false
creator: Sarah R. Sandock
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K318514
component: BBa_B0010
component: BBa_K112806
component: BBa_J23116
component: BBa_B0012
component: BBa_K112807
component: BBa_K318516
component: BBa_J23100
component: BBa_K112805
sequence annotation: BBa_J23100
sequence annotation: BBa_K318516
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K318514
sequence annotation: BBa_K112805
sequence annotation: BBa_K112806
sequence annotation: BBa_J23116
sequence annotation: BBa_K112807
sequence: https://synbiohub.org/public/igem/BBa_K318519_sequence/1

BBa_K318514
type: Component
persistent identity: component2086630
display id: component2086630
version: 1
top level: BBa_K318519
definition: BBa_K318514
access: public

BBa_B0010
type: Component
persistent identity: component2086635
display id: component2086635
version: 1
top level: BBa_K318519
definition: BBa_B0010
access: public

BBa_K112806
type: Component
persistent identity: component2086634
display id: component2086634
version: 1
top level: BBa_K318519
definition: BBa_K112806
access: public

BBa_J23116
type: Component
persistent identity: component2086641
display id: component2086641
version: 1
top level: BBa_K318519
definition: BBa_J23116
access: public

BBa_B0012
type: Component
persistent identity: component2086637
display id: component2086637
version: 1
top level: BBa_K318519
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2086644
display id: component2086644
version: 1
top level: BBa_K318519
definition: BBa_B0010
access: public

BBa_K112807
type: Component
persistent identity: component2086643
display id: component2086643
version: 1
top level: BBa_K318519
definition: BBa_K112807
access: public

BBa_K318516
type: Component
persistent identity: component2086623
display id: component2086623
version: 1
top level: BBa_K318519
definition: BBa_K318516
access: public

BBa_J23100
type: Component
persistent identity: component2086622
display id: component2086622
version: 1
top level: BBa_K318519
definition: BBa_J23100
access: public

BBa_B0012
type: Component
persistent identity: component2086626
display id: component2086626
version: 1
top level: BBa_K318519
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2086624
display id: component2086624
version: 1
top level: BBa_K318519
definition: BBa_B0010
access: public

BBa_K112805
type: Component
persistent identity: component2086632
display id: component2086632
version: 1
top level: BBa_K318519
definition: BBa_K112805
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2086622
display id: annotation2086622
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086622/range2086622/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K318519/annotation2086622/range2086622/1
type: Range
persistent identity: range2086622
display id: range2086622
version: 1
top level: BBa_K318519
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K318516
type: SequenceAnnotation
persistent identity: annotation2086623
display id: annotation2086623
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086623/range2086623/1
component: BBa_K318516

https://synbiohub.org/public/igem/BBa_K318519/annotation2086623/range2086623/1
type: Range
persistent identity: range2086623
display id: range2086623
version: 1
top level: BBa_K318519
direction: direction/reverse
start: 44
end: 390
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2086624
display id: annotation2086624
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086624/range2086624/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K318519/annotation2086624/range2086624/1
type: Range
persistent identity: range2086624
display id: range2086624
version: 1
top level: BBa_K318519
direction: direction/forward
start: 399
end: 478
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2086626
display id: annotation2086626
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086626/range2086626/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K318519/annotation2086626/range2086626/1
type: Range
persistent identity: range2086626
display id: range2086626
version: 1
top level: BBa_K318519
direction: direction/forward
start: 487
end: 527
orientation: inline

BBa_K318514
type: SequenceAnnotation
persistent identity: annotation2086630
display id: annotation2086630
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086630/range2086630/1
component: BBa_K318514

https://synbiohub.org/public/igem/BBa_K318519/annotation2086630/range2086630/1
type: Range
persistent identity: range2086630
display id: range2086630
version: 1
top level: BBa_K318519
direction: direction/reverse
start: 536
end: 1370
orientation: inline

BBa_K112805
type: SequenceAnnotation
persistent identity: annotation2086632
display id: annotation2086632
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086632/range2086632/1
component: BBa_K112805

https://synbiohub.org/public/igem/BBa_K318519/annotation2086632/range2086632/1
type: Range
persistent identity: range2086632
display id: range2086632
version: 1
top level: BBa_K318519
direction: direction/reverse
start: 1379
end: 2054
orientation: inline

BBa_K112806
type: SequenceAnnotation
persistent identity: annotation2086634
display id: annotation2086634
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086634/range2086634/1
component: BBa_K112806

https://synbiohub.org/public/igem/BBa_K318519/annotation2086634/range2086634/1
type: Range
persistent identity: range2086634
display id: range2086634
version: 1
top level: BBa_K318519
direction: direction/reverse
start: 2063
end: 2576
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2086635
display id: annotation2086635
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086635/range2086635/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K318519/annotation2086635/range2086635/1
type: Range
persistent identity: range2086635
display id: range2086635
version: 1
top level: BBa_K318519
direction: direction/forward
start: 2585
end: 2664
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2086637
display id: annotation2086637
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086637/range2086637/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K318519/annotation2086637/range2086637/1
type: Range
persistent identity: range2086637
display id: range2086637
version: 1
top level: BBa_K318519
direction: direction/forward
start: 2673
end: 2713
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2086641
display id: annotation2086641
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086641/range2086641/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K318519/annotation2086641/range2086641/1
type: Range
persistent identity: range2086641
display id: range2086641
version: 1
top level: BBa_K318519
direction: direction/reverse
start: 2722
end: 2756
orientation: inline

BBa_K112807
type: SequenceAnnotation
persistent identity: annotation2086643
display id: annotation2086643
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086643/range2086643/1
component: BBa_K112807

https://synbiohub.org/public/igem/BBa_K318519/annotation2086643/range2086643/1
type: Range
persistent identity: range2086643
display id: range2086643
version: 1
top level: BBa_K318519
direction: direction/reverse
start: 2765
end: 3075
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2086644
display id: annotation2086644
version: 1
top level: BBa_K318519
location: https://synbiohub.org/public/igem/BBa_K318519/annotation2086644/range2086644/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K318519/annotation2086644/range2086644/1
type: Range
persistent identity: range2086644
display id: range2086644
version: 1
top level: BBa_K318519
direction: direction/forward
start: 3084
end: 3163
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K318514
type: ComponentDefinition
persistent identity: BBa K318514
display id: BBa_K318514
version: 1
was generated by: iGEM to SBOL conversion
description: acrRA
created: 2010-09-25T11:00:00Z
modified: 2015-05-08T01:11:58Z
mutable provenance: uc
owned by: james
owned by: myers
mutable description: uc
discontinued: false
dominant: false
group u list: _438_
m user id: 0
owner id: 4504
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: uc
star: false
creator: Sarah R. Sandock
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K318514_sequence/1

BBa_K318516
type: ComponentDefinition
persistent identity: BBa K318516
display id: BBa_K318516
version: 1
was generated by: iGEM to SBOL conversion
description: RamA
created: 2010-09-25T11:00:00Z
modified: 2015-05-08T01:11:58Z
mutable provenance: uc
owned by: james
owned by: myers
mutable description: uc
discontinued: false
dominant: false
group u list: _438_
m user id: 0
owner id: 4504
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: uc
star: false
creator: Sarah R. Sandock
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K318516_sequence/1

BBa_K112805
type: ComponentDefinition
persistent identity: BBa K112805
display id: BBa_K112805
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 holin]
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts
owned by: james
owned by: myers
mutable description: Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer.
discontinued: false
dominant: true
experience: experience/Works
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 holin
sequence: https://synbiohub.org/public/igem/BBa_K112805_sequence/1

T4 holin
type: SequenceAnnotation
persistent identity: annotation1999005
display id: annotation1999005
version: 1
top level: BBa_K112805
location: https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K112805/annotation1999005/range1999005/1
type: Range
persistent identity: range1999005
display id: range1999005
version: 1
top level: BBa_K112805
direction: direction/forward
start: 17
end: 673
orientation: inline

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 endolysin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: In stock
status: status/Available
bookmark: false
mutable notes: High expression of lysozyme only is toxic to bacteria.
star: false
creator: Jin Huh
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
top level: BBa_K112806
direction: direction/forward
start: 17
end: 511
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_K112807
type: ComponentDefinition
persistent identity: BBa K112807
display id: BBa_K112807
version: 1
was generated by: iGEM to SBOL conversion
description: [T4 antiholin]
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=59202&to=59495&view=gbwithparts
owned by: james
owned by: myers
mutable description: Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria.
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Higher constitutive expression of antiholin compared to the expression of holin is required for the stable lysis device.
star: false
creator: Jin Huh
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 antiholin
sequence: https://synbiohub.org/public/igem/BBa_K112807_sequence/1

T4 antiholin
type: SequenceAnnotation
persistent identity: annotation1999019
display id: annotation1999019
version: 1
top level: BBa_K112807
location: https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K112807/annotation1999019/range1999019/1
type: Range
persistent identity: range1999019
display id: range1999019
version: 1
top level: BBa_K112807
direction: direction/forward
start: 15
end: 308
orientation: inline

https://synbiohub.org/public/igem/BBa_K318519_sequence/1
type: Sequence
persistent identity: BBa K318519 sequence
display id: BBa_K318519_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagaggatgaccatttccgctcaggttatcgacacgattgtcgagtggattgatgataatttgaatcagccgttacgtattgatgatattgcccgtcatgcggggtattccaagtggcacctgcagcgcctttttatgcagtacaaaggggagagtctgggacgctacgtgcgtgaacggaagctaaaactggcggcgcgcgacctgctcgacaccgaccagaaggtgtatgatatttgtctcaagtatggttttgattcgcagcaaacctttacgcgcatttttacccgcacgttcaacctgccgccaggcgcttatcgtaaagaaaagcatggccgtacgcattgaaatgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatcagggggagccgttgaccgtcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatcaaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtcagcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaaggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcggcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaattttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttatttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgccttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgccatatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacgcagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacagagggttaacgcctctggcggtcgtactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K318514_sequence/1
type: Sequence
persistent identity: BBa K318514 sequence
display id: BBa_K318514_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcagggggagccgttgaccgtcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatcaaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtcagcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaaggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcggcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaattttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttatttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgccttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgccatatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacgcagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacagagggttaacgcctctggcggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K318516_sequence/1
type: Sequence
persistent identity: BBa K318516 sequence
display id: BBa_K318516_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatgaccatttccgctcaggttatcgacacgattgtcgagtggattgatgataatttgaatcagccgttacgtattgatgatattgcccgtcatgcggggtattccaagtggcacctgcagcgcctttttatgcagtacaaaggggagagtctgggacgctacgtgcgtgaacggaagctaaaactggcggcgcgcgacctgctcgacaccgaccagaaggtgtatgatatttgtctcaagtatggttttgattcgcagcaaacctttacgcgcatttttacccgcacgttcaacctgccgccaggcgcttatcgtaaagaaaagcatggccgtacgcattgaaatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23116_sequence/1
type: Sequence
persistent identity: BBa J23116 sequence
display id: BBa_J23116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112805_sequence/1
type: Sequence
persistent identity: BBa K112805 sequence
display id: BBa_K112805_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112806_sequence/1
type: Sequence
persistent identity: BBa K112806 sequence
display id: BBa_K112806_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K112807_sequence/1
type: Sequence
persistent identity: BBa K112807 sequence
display id: BBa_K112807_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z