LOCUS BBa_K318521 5507 bp DNA linear 05-Nov-2024 DEFINITION Encryption Lysis - pLock Lysis p(pH) Hin pBile Cre pCons ramA ACCESSION BBa_K318521 VERSION BBa_K318521.1 FEATURES Location/Qualifiers misc_feature 1..34 /label=BBa_I718016 promoter 43..77 /label=BBa_K318050 misc_feature 86..111 /label=BBa_J44000 misc_feature 120..153 /label=BBa_I718017 misc_feature 162..172 /label=BBa_K318051 misc_feature 181..219 /label=B1006 stem_loop 190..211 /label=modified thr terminator polyA_site 212..219 /label=PolyA polyA_site 181..189 /label=PolyA terminator 181..219 /label=BBa_B1006 misc_feature 228..266 /label=B1006 stem_loop 237..258 /label=modified thr terminator polyA_site 259..266 /label=PolyA polyA_site 228..236 /label=PolyA terminator 228..266 /label=BBa_B1006 misc_feature 275..357 /label=BBa_K318052 misc_feature 366..391 /label=BBa_J44000 CDS 416..1072 /label=T4 holin CDS 400..1075 /label=BBa_K112805 CDS 1100..1594 /label=T4 Endolysin CDS 1084..1597 /label=BBa_K112806 stem_loop 1617..1660 /label=stem_loop misc_feature 1606..1685 /label=BBa_B0010 terminator 1606..1685 /label=BBa_B0010 stem_loop 1701..1720 /label=T7 TE misc_feature 1727..1727 /label=stop polyA_site 1721..1734 /label=polya misc_feature 1694..1734 /label=BBa_B0012 terminator 1694..1734 /label=BBa_B0012 promoter 1743..1777 /label=BBa_J23116 CDS 1800..2093 /label=T4 antiholin CDS 1786..2096 /label=BBa_K112807 stem_loop 2116..2159 /label=stem_loop misc_feature 2105..2184 /label=BBa_B0010 terminator 2105..2184 /label=BBa_B0010 misc_feature 2193..2231 /label=B1006 stem_loop 2202..2223 /label=modified thr terminator polyA_site 2224..2231 /label=PolyA polyA_site 2193..2201 /label=PolyA terminator 2193..2231 /label=BBa_B1006 promoter 2240..2503 /label=BBa_K318512 misc_feature 2579..2584 /label=Former SpeI site misc_feature 2581..2581 /label=Mutation to delete the SpeI site CDS 2510..3082 /label=Hin sequence primer_bind 2510..2512 /label=Fwd primer J31001 primer_bind 3060..3079 /label=Rev primer J31001 CDS 2510..3082 /label=BBa_J31000 misc_feature 3091..3129 /label=B1006 stem_loop 3100..3121 /label=modified thr terminator polyA_site 3122..3129 /label=PolyA polyA_site 3091..3099 /label=PolyA terminator 3091..3129 /label=BBa_B1006 promoter 3138..3972 /label=BBa_K318514 CDS 3979..5015 /label=BBa_J61047 misc_feature 5024..5062 /label=B1006 stem_loop 5033..5054 /label=modified thr terminator polyA_site 5055..5062 /label=PolyA polyA_site 5024..5032 /label=PolyA terminator 5024..5062 /label=BBa_B1006 promoter 5071..5105 /label=BBa_J23100 CDS 5114..5460 /label=BBa_K318516 misc_feature 5469..5507 /label=B1006 stem_loop 5478..5499 /label=modified thr terminator polyA_site 5500..5507 /label=PolyA polyA_site 5469..5477 /label=PolyA terminator 5469..5507 /label=BBa_B1006 ORIGIN 1 ataacttggt atagcataca ttatacgaac ggtatactag aggctagcac tgtacctagg 61 actgagctag ccgtcaatac tagagttatc aaaaaccatg gtttttgata atactagagt 121 accgttcgta tacgatacat tatacgaagt tattactaga ggctcttcaa tctactagag 181 aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagagaaa aaaaaacccc 241 gcccctgaca gggcggggtt tttttttact agagttcggg tgtcaacaat tgaccaaaat 301 attgatttac agcgtaatgc gctttctagt gcaaattgtg accgcatttt gaagctttac 361 tagagttatc aaaaaccatg gtttttgata atactagagc ttaaaaggag ggtctatggc 421 agcacctaga atatcatttt cgccctctga tattctattt ggtgttctcg atcgcttgtt 481 caaagataac gctaccggga aggttcttgc ttcccgggta gctgtcgtaa ttcttttgtt 541 tataatggcg attgtttggt ataggggaga tagtttcttt gagtactata agcaatcaaa 601 gtatgaaaca tacagtgaaa ttattgaaaa ggaaagaact gcacgctttg aatctgtcgc 661 cctggaacaa ctccagatag ttcatatatc atctgaggca gactttagtg cggtgtattc 721 tttccgccct aaaaacttaa actattttgt tgatattata gcatacgaag gaaaattacc 781 ttcaacaata agtgaaaaat cacttggagg atatcctgtt gataaaacta tggatgaata 841 tacagttcat ttaaatggac gtcattatta ttccaactca aaatttgctt ttttaccaac 901 taaaaagcct actcccgaaa taaactacat gtacagttgt ccatatttta atttggataa 961 tatctatgct ggaacgataa ccatgtactg gtatagaaat gatcatataa gtaatgaccg 1021 ccttgaatca atatgtgctc aggcggccag aatattagga agggctaaat aattatacta 1081 gagatactta ggaggtatta tgaatatatt tgaaatgtta cgtatagatg aaggtcttag 1141 acttaaaatc tataaagaca cagaaggcta ttacactatt ggcatcggtc atttgcttac 1201 aaaaagtcca tcacttaatg ctgctaaatc tgaattagat aaagctattg ggcgtaattg 1261 caatggtgta attacaaaag atgaggctga aaaactcttt aatcaggatg ttgatgctgc 1321 tgttcgcgga atcctgagaa atgctaaatt aaaaccggtt tatgattctc ttgatgcggt 1381 tcgtcgctgt gcattgatta atatggtttt ccaaatggga gaaaccggtg tggcaggatt 1441 tactaactct ttacgtatgc ttcaacaaaa acgctgggat gaagcagcag ttaacttagc 1501 taaaagtaga tggtataatc aaacacctaa tcgcgcaaaa cgagtcatta caacgtttag 1561 aactggcact tgggacgcgt ataaaaatct ataaagctac tagagccagg catcaaataa 1621 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 1681 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 1741 agttgacagc tagctcagtc ctagggacta tgctagctac tagaggatag gaggccttta 1801 tggccttaaa agcaacagca ctttttgcca tgctaggatt gtcatttgtt ttatctccat 1861 cgattgaagc gaatgtcgat cctcattttg ataaatttat ggaatctggt attaggcacg 1921 tttatatgct ttttgaaaat aaaagcgtag aatcgtctga acaattctat agttttatga 1981 gaacgaccta taaaaatgac ccgtgctctt ctgattttga atgtatagag cgaggcgcgg 2041 agatggcaca atcatacgct agaattatga acattaaatt ggagactgaa tgaaattact 2101 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2161 tgttgtttgt cggtgaacgc tctctactag agaaaaaaaa accccgcccc tgacagggcg 2221 gggttttttt ttactagagc tttcctgcac agtctgaacg gggtgtacac gccgtatatg 2281 caggataaac aataattaat ttgatcgccc gaacagcaat gtttgggcga tttttattac 2341 gataataaag tctgttttta atattatcat gttaaatgtt tatattataa aaagtcgttt 2401 ttctgcttag gattttgtta tttaaattaa gcctgtaatg ccttgcttcc attgcggata 2461 aatcctactt ttttattgcc ttcaaataaa tttaaggagt tcgtactaga tggctactat 2521 tgggtatatt cgggtgtcaa caattgacca aaatatcgat ttacagcgta atgcgcttac 2581 cagtgcaaat tgtgaccgca tttttgaaga ccgtatcagt ggcaagattg caaaccgccc 2641 cggcctgaaa cgggcgttaa agtatgtaaa taaaggcgat actcttgtcg tctggaaatt 2701 agacagactg ggccgtagcg tgaaaaatct ggtggcgtta atatcagaat tacatgaacg 2761 tggagctcac ttccattctt taaccgatag tattgatacc agtagcgcga tggggcgatt 2821 cttttttcat gtaatgtcag cactggccga gatggagcga gaattaatcg tcgagcgaac 2881 ccttgccgga ctggctgccg ccagagcgca aggacgactg ggagggcgcc ctcgggcgat 2941 caacaaacat gaacaggaac agattagtcg gctattagag aaaggccatc ctcggcagca 3001 attagctatt atttttggta ttggcgtatc caccttatac agatactttc cggcaagcag 3061 tataaaaaaa cgaatgaatt aatactagag aaaaaaaaac cccgcccctg acagggcggg 3121 gttttttttt actagagtta tcagggggag ccgttgaccg tcgacgcgcg cagcgtcgga 3181 cacaattgat acatctccag caggatcgtg acgtaggcgc gagcttcttt ttttaaatca 3241 aacgattgcg gagcaaataa ccagttttcc atgagccctg aaataaagct gcgcatcagt 3301 atcgccgcac gacgggtcag cagattttca ggcagcattt tagcattaat acaatgtttt 3361 aacgtttgtt caatccgatc gtaactttcc agacaaaggc tacgttgcgc ctgttgaacc 3421 acaaccattt ctccgacaaa ctcacattta tggaatataa tctccatcaa taagcggcgt 3481 cgttcttctg ttacagtagc ttcaagaata tgaactaaaa tttctcttaa tacagatagt 3541 ggatcgtcgg ggaattttgc ctgatactca atctcaagct caccaatatt ggattctgat 3601 agctcccaga tctcactgaa taaatccgac ttatttttga aatgccaata gattgcgccg 3661 cgcgtcacgc cagcagcgtt cgcaatctcc gccagcgagg ttgccgatac gccttgctgc 3721 gagaacaaac gcagggccac atccaggatg tgttgtcgtg tctccagcgc ttgttgtttg 3781 gtttttcgtg ccatatgtcg gtgaatttac aggcgttaga tttacataca tttatggatg 3841 tatgtaccat agcacgacga taatataaac gcagcaatgg gtttaaggac ctttgaccat 3901 tgaccaattt gaaatcggac actcgaggtt tacatatgaa caaaaacaga gggttaacgc 3961 ctctggcggt cgtactagat gtccaattta ctgaccgtac accaaaattt gcctgcatta 4021 ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc 4081 caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca 4141 tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat 4201 cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag 4261 ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca 4321 ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct 4381 ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc 4441 tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata 4501 gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta 4561 atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc 4621 ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg 4681 aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc 4741 cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc 4801 gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga 4861 gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc 4921 tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca 4981 atggtgcgcc tgctggaaga tggcgattaa gaatttacta gagaaaaaaa aaccccgccc 5041 ctgacagggc ggggtttttt tttactagag ttgacggcta gctcagtcct aggtacagtg 5101 ctagctacta gaggatgacc atttccgctc aggttatcga cacgattgtc gagtggattg 5161 atgataattt gaatcagccg ttacgtattg atgatattgc ccgtcatgcg gggtattcca 5221 agtggcacct gcagcgcctt tttatgcagt acaaagggga gagtctggga cgctacgtgc 5281 gtgaacggaa gctaaaactg gcggcgcgcg acctgctcga caccgaccag aaggtgtatg 5341 atatttgtct caagtatggt tttgattcgc agcaaacctt tacgcgcatt tttacccgca 5401 cgttcaacct gccgccaggc gcttatcgta aagaaaagca tggccgtacg cattgaaatg 5461 tactagagaa aaaaaaaccc cgcccctgac agggcggggt ttttttt //