##gff-version 3
##sequence-region BBa_K318521 1 5507
BBa_K318521	.	promoter	5071	5105	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K318521	.	terminator	3091	3129	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K318521	.	polyA_site	3122	3129	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	stem_loop	3100	3121	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K318521	.	polyA_site	3091	3099	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	engineered_region	3091	3129	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K318521	.	terminator	2105	2184	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K318521	.	engineered_region	2105	2184	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K318521	.	stem_loop	2116	2159	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K318521	.	CDS	1084	1597	.	+	0	ID=BBa_K112806;Name=BBa_K112806
BBa_K318521	.	CDS	1100	1594	.	+	0	ID=annotation1999018;Name=T4 Endolysin;Parent=BBa_K112806
BBa_K318521	.	terminator	2193	2231	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K318521	.	polyA_site	2224	2231	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	stem_loop	2202	2223	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K318521	.	polyA_site	2193	2201	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	engineered_region	2193	2231	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K318521	.	terminator	181	219	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K318521	.	polyA_site	212	219	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	stem_loop	190	211	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K318521	.	polyA_site	181	189	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	engineered_region	181	219	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K318521	.	CDS	5114	5460	.	+	0	ID=BBa_K318516;Name=BBa_K318516
BBa_K318521	.	promoter	2240	2503	.	+	0	ID=BBa_K318512;Name=BBa_K318512
BBa_K318521	.	sequence_feature	120	153	.	+	0	ID=BBa_I718017;Name=BBa_I718017
BBa_K318521	.	CDS	3979	5015	.	+	0	ID=BBa_J61047;Name=BBa_J61047
BBa_K318521	.	sequence_feature	275	357	.	+	0	ID=BBa_K318052;Name=BBa_K318052
BBa_K318521	.	sequence_feature	162	172	.	+	0	ID=BBa_K318051;Name=BBa_K318051
BBa_K318521	.	terminator	5024	5062	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K318521	.	polyA_site	5055	5062	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	stem_loop	5033	5054	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K318521	.	polyA_site	5024	5032	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	engineered_region	5024	5062	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K318521	.	promoter	1743	1777	.	+	0	ID=BBa_J23116;Name=BBa_J23116
BBa_K318521	.	terminator	1606	1685	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K318521	.	engineered_region	1606	1685	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K318521	.	stem_loop	1617	1660	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K318521	.	CDS	2510	3082	.	+	0	ID=BBa_J31000;Name=BBa_J31000
BBa_K318521	.	CDS	2510	3082	.	+	0	ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000
BBa_K318521	.	sequence_alteration	2581	2581	.	+	0	ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000
BBa_K318521	.	sequence_alteration	2579	2584	.	+	0	ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000
BBa_K318521	.	primer_binding_site	3060	3079	.	+	0	ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000
BBa_K318521	.	primer_binding_site	2510	2512	.	+	0	ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000
BBa_K318521	.	CDS	1786	2096	.	+	0	ID=BBa_K112807;Name=BBa_K112807
BBa_K318521	.	CDS	1800	2093	.	+	0	ID=annotation1999019;Name=T4 antiholin;Parent=BBa_K112807
BBa_K318521	.	sequence_feature	1	34	.	+	0	ID=BBa_I718016;Name=BBa_I718016
BBa_K318521	.	sequence_feature	86	111	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_K318521	.	promoter	3138	3972	.	+	0	ID=BBa_K318514;Name=BBa_K318514
BBa_K318521	.	terminator	228	266	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K318521	.	polyA_site	259	266	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	stem_loop	237	258	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K318521	.	polyA_site	228	236	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	engineered_region	228	266	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K318521	.	CDS	400	1075	.	+	0	ID=BBa_K112805;Name=BBa_K112805
BBa_K318521	.	CDS	416	1072	.	+	0	ID=annotation1999005;Name=T4 holin;Parent=BBa_K112805
BBa_K318521	.	sequence_feature	366	391	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_K318521	.	terminator	1694	1734	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K318521	.	engineered_region	1694	1734	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K318521	.	polyA_site	1721	1734	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K318521	.	stop_codon	1727	1727	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K318521	.	stem_loop	1701	1720	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K318521	.	promoter	43	77	.	+	0	ID=BBa_K318050;Name=BBa_K318050
BBa_K318521	.	terminator	5469	5507	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K318521	.	polyA_site	5500	5507	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	stem_loop	5478	5499	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K318521	.	polyA_site	5469	5477	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K318521	.	engineered_region	5469	5507	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
>BBa_K318521
ataacttggtatagcatacattatacgaacggtatactagaggctagcactgtacctaggactgagctagccgtcaata
ctagagttatcaaaaaccatggtttttgataatactagagtaccgttcgtatacgatacattatacgaagttattacta
gaggctcttcaatctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaaaaaaaaac
cccgcccctgacagggcggggtttttttttactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgta
atgcgctttctagtgcaaattgtgaccgcattttgaagctttactagagttatcaaaaaccatggtttttgataatact
agagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcg
cttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgatt
gtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaagg
aaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgc
ggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaata
agtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattatt
attccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattt
taatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaa
tcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaata
tatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcat
cggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaat
ggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatg
ctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggaga
aaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagct
aaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgt
ataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact
agagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaaca
gcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaat
ttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttt
tatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatca
tacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaaaaaaaaaccccgcccctg
acagggcggggtttttttttactagagctttcctgcacagtctgaacggggtgtacacgccgtatatgcaggataaaca
ataattaatttgatcgcccgaacagcaatgtttgggcgatttttattacgataataaagtctgtttttaatattatcat
gttaaatgtttatattataaaaagtcgtttttctgcttaggattttgttatttaaattaagcctgtaatgccttgcttc
cattgcggataaatcctacttttttattgccttcaaataaatttaaggagttcgtactagatggctactattgggtata
ttcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttga
agaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactctt
gtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggag
ctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcact
ggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactggga
gggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaat
tagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaatta
atactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagttatcagggggagccgttgaccg
tcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatc
aaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtc
agcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaa
ggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcg
gcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaat
tttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttat
ttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgcc
ttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgcc
atatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacg
cagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacaga
gggttaacgcctctggcggtcgtactagatgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgat
gcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaa
tgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaaga
tgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaac
atgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaag
aaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcat
ggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagcc
gaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaa
cgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctc
tggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagc
cagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtc
agagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaatacc
ggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggg
gcaatggtgcgcctgctggaagatggcgattaagaatttactagagaaaaaaaaaccccgcccctgacagggcggggtt
tttttttactagagttgacggctagctcagtcctaggtacagtgctagctactagaggatgaccatttccgctcaggtt
atcgacacgattgtcgagtggattgatgataatttgaatcagccgttacgtattgatgatattgcccgtcatgcggggt
attccaagtggcacctgcagcgcctttttatgcagtacaaaggggagagtctgggacgctacgtgcgtgaacggaagct
aaaactggcggcgcgcgacctgctcgacaccgaccagaaggtgtatgatatttgtctcaagtatggttttgattcgcag
caaacctttacgcgcatttttacccgcacgttcaacctgccgccaggcgcttatcgtaaagaaaagcatggccgtacgc
attgaaatgtactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt