BBa_K112805 1 BBa_K112805 [T4 holin] 2008-10-30T12:00:00Z 2015-05-08T01:09:19Z Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. false true _224_ 0 3025 171 It's complicated false Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin. false Jin Huh annotation1999005 1 T4 holin range1999005 1 17 673 BBa_K318052 1 BBa_K318052 RE+HindIII 2010-08-26T11:00:00Z 2015-05-08T01:11:58Z 42 BBa_J3101 with a HindIII site added to the end. false false _438_ 0 4845 9 Not in stock false None false Nathaniel Pantalone BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898431 1 PolyA range1898431 1 1 9 annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 annotation1898429 1 modified thr terminator range1898429 1 10 31 BBa_K112806 1 BBa_K112806 [T4 endolysin] 2008-10-31T12:00:00Z 2015-05-08T01:09:19Z Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts Released HQ 2013 The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer. false true _224_ 0 3025 171 In stock false High expression of lysozyme only is toxic to bacteria. false Jin Huh annotation1999018 1 T4 Endolysin range1999018 1 17 511 BBa_J31000 1 Hin DNA-invertase Hin from Salmonella typhimurium 2006-06-04T11:00:00Z 2015-08-31T04:08:45Z Salmonella typhimurium Generates the Salmonella typhimurium Hin protein. This protein is used to invert DNA that is flanked by Hix siteds. false false _61_ 0 918 61 It's complicated false none really false Erin Zwack, Sabriya Rosemond annotation1884987 1 Hin sequence range1884987 1 1 573 annotation1880459 1 Former SpeI site range1880459 1 70 75 annotation1910569 1 Rev primer J31001 range1910569 1 551 570 annotation1910568 1 Fwd primer J31001 range1910568 1 1 3 annotation1880460 1 Mutation to delete the SpeI site range1880460 1 72 72 BBa_K318512 1 gadA gadA (pH promoter and RBS) 2010-07-21T11:00:00Z 2015-05-08T01:11:58Z uc uc false false _438_ 0 4504 9 In stock true uc false Sarah R. Sandock BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K318051 1 BBa_K318051 SapI site 2010-08-26T11:00:00Z 2015-05-08T01:11:58Z Life SapI site for junk DNA addition by multimerization of oligonucleotides. false false _438_ 0 4845 9 Not in stock false The three bases that make up the sticky ends after digestion are ATC false Nathaniel Pantalone BBa_I718016 1 lox66 lox66 2007-10-25T11:00:00Z 2015-08-31T04:07:52Z This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed lox66 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularily in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox66 and a lox71 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox66 & lox71 sites is potentially interresting when the recombination reaction must be "irreversible". false false _141_ 0 1568 9 In stock false No modidification was made on the lox66 sequence true Eimad Shotar BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K112807 1 BBa_K112807 [T4 antiholin] 2008-10-31T12:00:00Z 2015-05-08T01:09:19Z Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=59202&to=59495&view=gbwithparts Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria. false true _224_ 0 3025 171 It's complicated false Higher constitutive expression of antiholin compared to the expression of holin is required for the stable lysis device. false Jin Huh annotation1999019 1 T4 antiholin range1999019 1 15 308 BBa_J61047 1 Cre Cre DNA recombinase 2007-02-20T12:00:00Z 2015-08-31T02:03:00Z bob bob false false _95_ 0 483 95 In stock false bob true John Anderson BBa_J44000 1 hixC hixC binding site for Salmonella typhimurium Hin recombinase 2006-06-05T11:00:00Z 2015-08-31T04:08:48Z Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649] A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome. false true _71_ 0 606 61 In stock true Standard BioBrick prefix and suffix were added to the 26 bp sequence. true Missouri Western and Davidson Groups, Todd Eckdahl BBa_K318521 1 BBa_K318521 Encryption Lysis - pLock Lysis p(pH) Hin pBile Cre pCons ramA 2010-10-17T11:00:00Z 2015-05-08T01:11:58Z Registry parts Where to start... false false _438_ 0 4845 9 Not in stock true All theoretical false Nathaniel Pantalone component2089206 1 BBa_K318516 component2089192 1 BBa_J31000 component2089152 1 BBa_I718017 component2089199 1 BBa_J61047 component2089205 1 BBa_J23100 component2089151 1 BBa_J44000 component2089198 1 BBa_K318514 component2089158 1 BBa_B1006 component2089164 1 BBa_K318052 component2089153 1 BBa_K318051 component2089169 1 BBa_K112806 component2089149 1 BBa_I718016 component2089167 1 BBa_K112805 component2089150 1 BBa_K318050 component2089197 1 BBa_B1006 component2089163 1 BBa_B1006 component2089211 1 BBa_B1006 component2089178 1 BBa_K112807 component2089170 1 BBa_B0010 component2089186 1 BBa_K318512 component2089204 1 BBa_B1006 component2089165 1 BBa_J44000 component2089176 1 BBa_J23116 component2089185 1 BBa_B1006 component2089172 1 BBa_B0012 component2089179 1 BBa_B0010 annotation2089178 1 BBa_K112807 range2089178 1 1786 2096 annotation2089204 1 BBa_B1006 range2089204 1 5024 5062 annotation2089176 1 BBa_J23116 range2089176 1 1743 1777 annotation2089167 1 BBa_K112805 range2089167 1 400 1075 annotation2089165 1 BBa_J44000 range2089165 1 366 391 annotation2089198 1 BBa_K318514 range2089198 1 3138 3972 annotation2089151 1 BBa_J44000 range2089151 1 86 111 annotation2089158 1 BBa_B1006 range2089158 1 181 219 annotation2089169 1 BBa_K112806 range2089169 1 1084 1597 annotation2089185 1 BBa_B1006 range2089185 1 2193 2231 annotation2089199 1 BBa_J61047 range2089199 1 3979 5015 annotation2089163 1 BBa_B1006 range2089163 1 228 266 annotation2089205 1 BBa_J23100 range2089205 1 5071 5105 annotation2089164 1 BBa_K318052 range2089164 1 275 357 annotation2089152 1 BBa_I718017 range2089152 1 120 153 annotation2089170 1 BBa_B0010 range2089170 1 1606 1685 annotation2089149 1 BBa_I718016 range2089149 1 1 34 annotation2089192 1 BBa_J31000 range2089192 1 2510 3082 annotation2089206 1 BBa_K318516 range2089206 1 5114 5460 annotation2089211 1 BBa_B1006 range2089211 1 5469 5507 annotation2089153 1 BBa_K318051 range2089153 1 162 172 annotation2089172 1 BBa_B0012 range2089172 1 1694 1734 annotation2089197 1 BBa_B1006 range2089197 1 3091 3129 annotation2089179 1 BBa_B0010 range2089179 1 2105 2184 annotation2089186 1 BBa_K318512 range2089186 1 2240 2503 annotation2089150 1 BBa_K318050 range2089150 1 43 77 BBa_K318516 1 BBa_K318516 RamA 2010-09-25T11:00:00Z 2015-05-08T01:11:58Z uc uc false false _438_ 0 4504 9 Not in stock false uc false Sarah R. Sandock BBa_I718017 1 lox71 lox71 2007-10-25T11:00:00Z 2015-08-31T04:07:52Z This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed Released HQ 2013 Lox71 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularly in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox71 and a lox66 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox71 & lox66 sites is potentially interesting when the recombination reaction must be "irreversible". false false _141_ 0 1568 9 In stock false No modifaication was made on lox71 sequence true Eimad Shotar BBa_K318050 1 BBa_K318050 rPcons (J23100) 2010-08-26T11:00:00Z 2015-05-08T01:11:58Z Registry The reverse compliment of BBa_J23100 false false _438_ 0 4845 9 Not in stock false This part expresses very strongly. Pick smaller colonies when screening. false Nathaniel Pantalone BBa_K318514 1 BBa_K318514 acrRA 2010-09-25T11:00:00Z 2015-05-08T01:11:58Z uc uc false false _438_ 0 4504 9 Not in stock false uc false Sarah R. Sandock BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23116 1 BBa_J23116 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_K318516_sequence 1 gatgaccatttccgctcaggttatcgacacgattgtcgagtggattgatgataatttgaatcagccgttacgtattgatgatattgcccgtcatgcggggtattccaagtggcacctgcagcgcctttttatgcagtacaaaggggagagtctgggacgctacgtgcgtgaacggaagctaaaactggcggcgcgcgacctgctcgacaccgaccagaaggtgtatgatatttgtctcaagtatggttttgattcgcagcaaacctttacgcgcatttttacccgcacgttcaacctgccgccaggcgcttatcgtaaagaaaagcatggccgtacgcattgaaatg BBa_K112805_sequence 1 cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta BBa_K318052_sequence 1 ttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttgaagctt BBa_I718017_sequence 1 taccgttcgtatacgatacattatacgaagttat BBa_K318512_sequence 1 ctttcctgcacagtctgaacggggtgtacacgccgtatatgcaggataaacaataattaatttgatcgcccgaacagcaatgtttgggcgatttttattacgataataaagtctgtttttaatattatcatgttaaatgtttatattataaaaagtcgtttttctgcttaggattttgttatttaaattaagcctgtaatgccttgcttccattgcggataaatcctacttttttattgccttcaaataaatttaaggagttcg BBa_J31000_sequence 1 atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaa BBa_K318050_sequence 1 gctagcactgtacctaggactgagctagccgtcaa BBa_K318051_sequence 1 gctcttcaatc BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_J23116_sequence 1 ttgacagctagctcagtcctagggactatgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K318514_sequence 1 ttatcagggggagccgttgaccgtcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatcaaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtcagcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaaggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcggcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaattttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttatttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgccttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgccatatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacgcagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacagagggttaacgcctctggcggtcg BBa_K318521_sequence 1 ataacttggtatagcatacattatacgaacggtatactagaggctagcactgtacctaggactgagctagccgtcaatactagagttatcaaaaaccatggtttttgataatactagagtaccgttcgtatacgatacattatacgaagttattactagaggctcttcaatctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttgaagctttactagagttatcaaaaaccatggtttttgataatactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagctttcctgcacagtctgaacggggtgtacacgccgtatatgcaggataaacaataattaatttgatcgcccgaacagcaatgtttgggcgatttttattacgataataaagtctgtttttaatattatcatgttaaatgtttatattataaaaagtcgtttttctgcttaggattttgttatttaaattaagcctgtaatgccttgcttccattgcggataaatcctacttttttattgccttcaaataaatttaaggagttcgtactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagttatcagggggagccgttgaccgtcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatcaaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtcagcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaaggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcggcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaattttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttatttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgccttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgccatatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacgcagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacagagggttaacgcctctggcggtcgtactagatgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatttactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagttgacggctagctcagtcctaggtacagtgctagctactagaggatgaccatttccgctcaggttatcgacacgattgtcgagtggattgatgataatttgaatcagccgttacgtattgatgatattgcccgtcatgcggggtattccaagtggcacctgcagcgcctttttatgcagtacaaaggggagagtctgggacgctacgtgcgtgaacggaagctaaaactggcggcgcgcgacctgctcgacaccgaccagaaggtgtatgatatttgtctcaagtatggttttgattcgcagcaaacctttacgcgcatttttacccgcacgttcaacctgccgccaggcgcttatcgtaaagaaaagcatggccgtacgcattgaaatgtactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_J44000_sequence 1 ttatcaaaaaccatggtttttgataa BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_I718016_sequence 1 ataacttggtatagcatacattatacgaacggta BBa_K112807_sequence 1 gataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaat BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K112806_sequence 1 atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc BBa_J61047_sequence 1 atgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z