LOCUS       BBa_K318599             1354 bp    DNA     linear       14-Nov-2024
DEFINITION  lacI pL + RBS + RcsB + RBS + RcsA + TT
ACCESSION   BBa_K318599
VERSION     BBa_K318599.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=start
     CDS             82..561
                     /label=cds
     misc_feature    562..564
                     /label=stop
     misc_feature    565..567
                     /label=stop
     CDS             82..567
                     /label=BBa_K200002
     misc_feature    580..583
                     /label=conserved
     RBS             576..587
                     /label=BBa_B0034
     CDS             594..1217
                     /label=BBa_K137113
     stem_loop       1237..1280
                     /label=stem_loop
     misc_feature    1226..1305
                     /label=BBa_B0010
     terminator      1226..1305
                     /label=BBa_B0010
     stem_loop       1321..1340
                     /label=T7 TE
     misc_feature    1347..1347
                     /label=stop
     polyA_site      1341..1354
                     /label=polya
     misc_feature    1314..1354
                     /label=BBa_B0012
     terminator      1314..1354
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgacaaac ctgacactgg atgtaaacat tatcgatttc
      121 ccatcaatac ctgtggcgat gttgccgcac cgctgtagcc ctgaattgct caactacagc
      181 gtggcgaaat ttatcatgtg gcgtaaagag acggggcttt ctcctgttaa ccaaagccag
      241 acttttggcg tcgcctggga cgaccctgcc accaccgcac cggaagcgtt tcgctttgat
      301 atctgcggca gcgttagcga accgattccc gataatcgtt atggtgtgag caatggtgaa
      361 cttaccggtg gacgttatgc cgtggcccgc cacgttggcg agctggacga tatttcacac
      421 acggtatggg gcatcattcg ccactggctg cctgcaagcg gcgagaaaat gcgtaaagca
      481 ccgattctgt ttcactacac caatcttgcc gaaggggtga cagagcagcg actggaaacg
      541 gatgtttatg tgccgttggc gtgataatac tagagaaaga ggagaaatac tagatgtcaa
      601 cgattattat ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta
      661 gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt
      721 gtgattcaca gcgcccttca gtggtgttta ttaatgagga ctgtttcatc cacgatgctt
      781 ctaacagtca gcgtatcaag ctcatcatta atcaacatcc caatacgtta tttatcgttt
      841 ttatggcaat tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattga
      901 tcagttctaa atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa
      961 aagagacaac gataacctcg tttttaaata tgccgacgtt atcattgagc cgaaccgaat
     1021 cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga
     1081 atatcaaagc caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacgc
     1141 ataataaaca ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt
     1201 ttgtcaacat gcgctaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1261 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1321 ggctcacctt cgggtgggcc tttctgcgtt tata
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