BBa_K319015
1
BBa_K319015
yeast T14 Proximal Promoter
2010-10-19T11:00:00Z
2015-05-08T01:11:58Z
The part was amplified via colony PCR from a YPH500 strain of S. cerevisiae. The strain expressing the part was given to us by Tom Ellis.
The proximal T14 promoter was constructed with two tandem TetR operators in addition to the TATA box and start site of the GAL1 promoter. The yeast promoter is repressed by TetR (Tn10.B tetracycline repressor) and inducible with the TetR-inhibitor andhydrotetracylcine (ATc). The promoter was constructed with runs of unspecified ("N") sequence separating motifs and fixed tandem TetR operator and GAL1 motifs. The unspecified motifs modulate the efficiency of the fixed motifs to fine tune the expression of the downstream protein coding region.
false
false
_437_
0
3073
9
It's complicated
false
The part was designed using primers that introduced standard 20 bp primer binding sites flanking the upstream and downstream regions of the part.
false
Matthew Orton
BBa_K319015_sequence
1
cctctcctttttgaaatattatgcaggaaaggataaacaactagagtgcagttacagaccatataaagaaaagttcaatccctatcagtgatagagaaatccctatcagtgatagagatccacccgataccacatccaaactaaaagtatcaacaaaaaattcaaaaccccactgccccaccagaccttgcttcacaccacccccggatctatcccgccgccaccatggag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z