LOCUS       BBa_K319043             2508 bp    DNA     linear       25-Nov-2024
DEFINITION  ADE4 targeting vector
ACCESSION   BBa_K319043
VERSION     BBa_K319043.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=EcoRI/MfeI mixed site
     misc_feature    7..206
                     /label=ATM1 terminator
     misc_feature    227..232
                     /label=EcoRI
     misc_feature    233..247
                     /label=G5C5G5 filler site
     misc_feature    248..253
                     /label=PstI site
     primer_bind     207..226
                     /label=Forward landing pad
     primer_bind     254..273
                     /label=Reverse landing pad
     misc_feature    274..473
                     /label=ADE4 terminator
     misc_feature    474..479
                     /label=PstI/NsiI mixed site
     misc_feature    1620..2279
                     /label=Cm resistance
ORIGIN
        1 gaattgaacg ctcgtaagta aatattgatt tatacatgta taagtttttc aagatttaag
       61 gaaaaaagaa aagttattat ctttctccgc ggtttttcaa tgacccgatg tattgactct
      121 tcctgaccga aaataacagg acagagttag aacgaacatg aataataaaa tataatctgg
      181 gatcatgaat ccgaatgact gctcatagta acaaccaggc agaagcgaat tcgggggccc
      241 ccgggggctg cagggtgact ggcctttgtc tgaatagatt gttccgaact tgttcagttg
      301 tatgtaaaat agtttccaaa tgcgcccatt tatacataat tatctttgta ttctgaccta
      361 aactaaatat atatatatat atgcacgtat taaaaaaaaa aataaaagca aaaaggtaaa
      421 caaagagaag taatccgcaa cttgtacaaa aagaaaagaa gataaaagaa ttgatgcagt
      481 ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
      541 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     1201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1381 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1441 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1501 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1561 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1621 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1681 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1741 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1801 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     1861 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1921 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1981 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     2041 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     2101 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     2161 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2221 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     2281 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     2341 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacttga
     2401 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     2461 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctg
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