BBa_K319043 1 BBa_K319043 ADE4 targeting vector 2010-10-25T11:00:00Z 2015-05-08T01:11:59Z n This is a targeting vector for homologous recombination into the ADE4 locus of S. cerevisiae. This vector can be used in cloning free cloning (ref. 2010 uOttawa iGEM wiki). Yeast strains with an ADE1 or ADE2 deletion or mutation have red colonies; by integrating the constructs using our protocol the ADE4 gene is knocked out and the colonies express a white phenotype. false false _437_ 0 7363 9 It's complicated true N/A false Afnan Azizi annotation2109295 1 PstI site range2109295 1 248 253 annotation2109297 1 Reverse landing pad range2109297 1 254 273 annotation2109301 1 PstI/NsiI mixed site range2109301 1 474 479 annotation2109294 1 G5C5G5 filler site range2109294 1 233 247 annotation2109296 1 Forward landing pad range2109296 1 207 226 annotation2109321 1 Cm resistance range2109321 1 1620 2279 annotation2109292 1 ATM1 terminator range2109292 1 7 206 annotation2109290 1 EcoRI/MfeI mixed site range2109290 1 1 6 annotation2109293 1 EcoRI range2109293 1 227 232 annotation2109298 1 ADE4 terminator range2109298 1 274 473 BBa_K319043_sequence 1 gaattgaacgctcgtaagtaaatattgatttatacatgtataagtttttcaagatttaaggaaaaaagaaaagttattatctttctccgcggtttttcaatgacccgatgtattgactcttcctgaccgaaaataacaggacagagttagaacgaacatgaataataaaatataatctgggatcatgaatccgaatgactgctcatagtaacaaccaggcagaagcgaattcgggggcccccgggggctgcagggtgactggcctttgtctgaatagattgttccgaacttgttcagttgtatgtaaaatagtttccaaatgcgcccatttatacataattatctttgtattctgacctaaactaaatatatatatatatatgcacgtattaaaaaaaaaaataaaagcaaaaaggtaaacaaagagaagtaatccgcaacttgtacaaaaagaaaagaagataaaagaattgatgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacttgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z