LOCUS       BBa_K320008             3505 bp    DNA     linear       13-Jun-2024
DEFINITION  Constitutive expression of Immunotoxin in Asaia and resistance to 
            Kanamycine
ACCESSION   BBa_K320008
VERSION     BBa_K320008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1131
                     /label=BBa_K320006
     promoter        1..84
                     /label=Strong Promotor
     CDS             85..1047
                     /label=Immunotoxine
     misc_feature    1132..3505
                     /label=BBa_K320004
     misc_feature    1486..2160
                     /label=Asaia recplication origin
     CDS             2531..3505
                     /label=KanR
ORIGIN
        1 ctagagaata cttgacatat cactgtgatt cacatataat atgcgcatcc tcttgataat
       61 tgctagcctt aagaaggagg aatactagat gaaatatctg ctgcctacag ctgctgctgg
      121 tctgctgctg ctggctgctc aacctgctat ggcaatggac atcggcatta acagtgatcc
      181 ggactataaa gacgacgacg ataaaatctc cgaagttcag ctgcaacagt ctggtgccga
      241 actggttcgt tctggagcaa gtgtacaact gtcctgtacc gcttctggct ttaacatcaa
      301 agacttctat atccactggg tgaaacagcg cccggaacaa ggactggaat ggattgggtg
      361 tatcgaccct gaatctgggg atgtggagta tgccccgaaa tttcaagaca aagcgactat
      421 gaccactgat acgagtagca ataccgccta tctgcaactg tctggcctga catctggtga
      481 cacagccgtg tatttttgta acgctctgat cctgcgtttt tctagcgcta tggactattg
      541 gggacgtggt acgagtgtaa ccgtttcttc aacaggtggt ggtggttctg gtggtggagg
      601 ttctggagga ggtggtagcg atatcgatgt gctgatgacc caaacacctc tgtctctgcc
      661 tattagcctg ggcgatcaag caagcatttc ttgccgttcc tctcagtcaa tcgttcacag
      721 caacggaaac acatatctgg aatggtatct gcaacgccct ggacaatctc ctaaactgct
      781 gctgttcaaa gtgagcaacc gctttagcgg tgttcctgat cgtttttccg gttctggcag
      841 tggcactgac tttacgctga aaatcacccg tgtggaagca gaagatctgg gggtctatta
      901 ttgttttcaa ggctcccatc tgccgtatac ctttggcggg ggtacgaaac tggaaatcaa
      961 acgtggtcct ggaggtccta tgcctcgttg gcgtctgttc cgtcgtatcg atcgtgttgg
     1021 caaacaaatc aaacagggca ttctgcgtgc aggtccggca attgctctgg ttggagatgc
     1081 tcgtgctgtc ggtgctgccg ctctggaaca tcatcaccat caccattaat actagagacc
     1141 ttgggcaagg ccgaaggccg cgcagtcgat caacaagccc cggaggggcc actttttgcc
     1201 ggagggggag ccgcgccgaa ggcgtggggg aaccccgcag gggtgccctt ctttgggcac
     1261 caaagaacta gatatagggc gaaatgcgaa agacttaaaa atcaacaact taaaaaaggg
     1321 gggtacgcaa cagctcattg cggcaccccc cgcaatagct cattgcgtag gttaaagaaa
     1381 atctgtaatt gactgccact tttacgcaac gcataattgt tgtcgcgctg ccgaaaagtt
     1441 gcagctgatt gcgcatggtg ccgcaaccgt gcggcaccct accgcatgga gataagcatg
     1501 gccacgcagt ccagagaaat cggcattcaa gccaagaaca agcccggtca ctgggtgcaa
     1561 acggaacgca aagcgcatga ggcgtgggcc gggcttattg cgaggaaacc cacggcggca
     1621 atgctgctgc atcacctcgt ggcgcagatg ggccaccaga acgccgtggt ggtcagccag
     1681 aagacacttt ccaagctcat cggacgttct ttgcggacgg tccaatacgc agtcaaggac
     1741 ttggtggccg agcgctggat ctccgtcgtg aagctcaacg gccccggcac cgtgtcggcc
     1801 tacgtggtca atgaccgcgt ggcgtggggc cagccccgcg accagttgcg cctgtcggtg
     1861 ttcagtgccg ccgtggtggt tgatcacgac gaccaggacg aatcgctgtt ggggcatggc
     1921 gacctgcgcc gcatcccgac cctgtatccg ggcgagcagc aactaccgac cggccccggc
     1981 gaggagccgc ccagccagcc cggcattccg ggcatggaac cagacctgcc agccttgacc
     2041 gaaacggagg aatgggaacg gcgcgggcag cagcgcctgc cgatgcccga tgagccgtgt
     2101 tttctggacg atggcgagcc gttggagccg ccgacacggg tcacgctgcc gcgccggtag
     2161 cacttgggtt gcgcagcaac ccgtaagtgc gctgttccag actatcggct gtagccgcct
     2221 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac
     2281 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccgtttt tatcaggctc
     2341 tgggaggcag aataaatgat catatcgtca attattacct ccacggggag agcctgagca
     2401 aactggcctc aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg
     2461 gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg
     2521 tcgaatttta ctagagctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc
     2581 acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     2641 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     2701 gggaaacgtc ttgctcccgt ccgcgcttaa actccaacat ggacgctgat ttatatgggt
     2761 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     2821 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     2881 cagatgagat ggtccgtctc aactggctga cggagtttat gcctctcccg accatcaagc
     2941 attttatccg tactcctgat gatgcgtggt tactcaccac cgcgattcct gggaaaacag
     3001 ccttccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggccg
     3061 tgttcctgcg ccggttacat tcgattcctg tttgtaattg tccttttaac agcgatcgtg
     3121 tatttcgtct tgctcaggcg caatcacgca tgaataacgg tttggttgat gcgagtgatt
     3181 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cacaagctct
     3241 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     3301 ttgacgaggg gaaattaata ggttgtattg atgttggacg ggtcggaatc gcagaccgtt
     3361 accaggacct tgccattctt tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     3421 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     3481 tgctcgatga gtttttctaa taata
//