LOCUS       BBa_K320009             3458 bp    DNA     linear       26-Dec-2024
DEFINITION  Constitutive expression of P28 in Asaia and Resistance to 
            Tetracycline
ACCESSION   BBa_K320009
VERSION     BBa_K320009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..84
                     /label=BBa_K320002
     CDS             85..768
                     /label=P28
     misc_feature    769..3458
                     /label=BBa_K320003
     misc_feature    1123..1797
                     /label=Asaia replication origin
     CDS             2168..3456
                     /label=TetR
ORIGIN
        1 ctagagaata cttgacatat cactgtgatt cacatataat atgcgcatcc tcttgataat
       61 tgctagcctt aagaaggagg aatactagat gaatacatat tttaaggtac ttcttttttt
      121 atttattcaa ctttacataa cgttgaataa ggctcgggtt actgaaaata caatatgtaa
      181 atatggttat ttaattcaga tgagtaatca ttatgaatgt aagtgtattg aaggatatgt
      241 attaataaat gaggacacgt gtggaaaaaa agtagtctgt gataaagttg aaaattcatt
      301 taaagcttgt gatgaatacg cttactgttt cgatttagga aataagaata atgaaaaaca
      361 gataaaatgt atgtgcagaa cagaatatac tttaactgct ggagtatgtg ttcctaatgt
      421 ttgtcgagat aaagtatgtg gtaaaggaaa atgtatagta gatcctgcaa attctttaac
      481 acatacatgc tcatgcaata taggtaccat acttaaccag aataaattat gtgatataca
      541 aggtgataca ccatgttcat taaaatgtgc agaaaatgaa gtgtgtacat tagaaggaaa
      601 ttattataca tgtaaagaag atccttcatc taacggagga ggaaatactg tggaccaggc
      661 tgatacatca tatagtgtaa taaacggagt aaccctaaca cacgttctga ttgtatgctc
      721 aatatttatt aaattgttaa tacatcatca tcatcatcat cactaatact agagaccttg
      781 ggcaaggccg aaggccgcgc agtcgatcaa caagccccgg aggggccact ttttgccgga
      841 gggggagccg cgccgaaggc gtgggggaac cccgcagggg tgcccttctt tgggcaccaa
      901 agaactagat atagggcgaa atgcgaaaga cttaaaaatc aacaacttaa aaaagggggg
      961 tacgcaacag ctcattgcgg caccccccgc aatagctcat tgcgtaggtt aaagaaaatc
     1021 tgtaattgac tgccactttt acgcaacgca taattgttgt cgcgctgccg aaaagttgca
     1081 gctgattgcg catggtgccg caaccgtgcg gcaccctacc gcatggagat aagcatggcc
     1141 acgcagtcca gagaaatcgg cattcaagcc aagaacaagc ccggtcactg ggtgcaaacg
     1201 gaacgcaaag cgcatgaggc gtgggccggg cttattgcga ggaaacccac ggcggcaatg
     1261 ctgctgcatc acctcgtggc gcagatgggc caccagaacg ccgtggtggt cagccagaag
     1321 acactttcca agctcatcgg acgttctttg cggacggtcc aatacgcagt caaggacttg
     1381 gtggccgagc gctggatctc cgtcgtgaag ctcaacggcc ccggcaccgt gtcggcctac
     1441 gtggtcaatg accgcgtggc gtggggccag ccccgcgacc agttgcgcct gtcggtgttc
     1501 agtgccgccg tggtggttga tcacgacgac caggacgaat cgctgttggg gcatggcgac
     1561 ctgcgccgca tcccgaccct gtatccgggc gagcagcaac taccgaccgg ccccggcgag
     1621 gagccgccca gccagcccgg cattccgggc atggaaccag acctgccagc cttgaccgaa
     1681 acggaggaat gggaacggcg cgggcagcag cgcctgccga tgcccgatga gccgtgtttt
     1741 ctggacgatg gcgagccgtt ggagccgccg acacgggtca cgctgccgcg ccggtagcac
     1801 ttgggttgcg cagcaacccg taagtgcgct gttccagact atcggctgta gccgcctcgc
     1861 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     1921 gacctgaatg gaagccggcg gcacctcgct aacggattca ccgtttttat caggctctgg
     1981 gaggcagaat aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac
     2041 tggcctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     2101 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg
     2161 aattttacta gaggagattc tcatgtttga cagcttatca tcgataagct ttaatgcggt
     2221 agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     2281 ctcatcgtca ttctcggcac cgtcaccctg gacgctgtag gcataggctt ggttatgccg
     2341 gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gtattgccag tcactatggc
     2401 gtgctgcttg cgctctatgc gttgatgcaa tttctttgcg cacccgttct cggagccctg
     2461 tccgaccgct ttggccgccg tccagtcctg ctcgcttcgc tccttggagc cactatcgac
     2521 tacgcgatca tggcgaccac acccgtcctg tggattctct acgccggacg catcgtggcg
     2581 ggcatcacgg gtgccacagg tgcggttgct ggtgcctata tcgccgacat caccgatggg
     2641 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     2701 ggccccgtgg ccgggggact gttgggtgcc atctccttgc atgcaccatt ccttgcggcg
     2761 gcggtgctca acggcctcaa cctcctcctg ggctgcttcc ttatgcagga atcgcataag
     2821 ggagagcgcc gtccgatgcc cttgcgtgcc ttcaatccag tcagctcctt ccggtgggcg
     2881 cggggcatga ctatcgtcgc cgcacttatg actgttttct ttatcatgca actcgtagga
     2941 caggttccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     3001 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     3061 acgggccccg ccaccaaacg tttcggcgag aagcaggcca ttatcgcggg catggcggcc
     3121 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgcg gctggatggc cttccccatt
     3181 atgattcttc tcgcttccgg cggcatcggt atgcccgcgt tgcaggccat gctgtcccgc
     3241 caagtagatg acgaccatca gggacagctt caagggtcgc tcgcggctct taccagcctc
     3301 acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     3361 aacgggttgg catggattgt aggtgccgcc ctttaccttg tctgcctccc cgcgttgcgt
     3421 cgcggtgcat ggagccgggc cacctcgacc taataata
//