BBa_K320014 1 S + P25 + S + P25 + P28 + Ori + Tet 2010-10-23T11:00:00Z 2015-05-08T01:11:59Z This part has been designed from parts we made. Constitutive expression of P25 and P28 with an origin fro Asaia and E.Coli and a resiatance to Tetrafenicol in Asaia This BioBricks makes the bacteria resitance to Tetracycline, contain an origin of replication for Asaia/E.Coli and a constitutice expression of the protein P28. The ookinete (plasmodium state in mosquito's gut) has been intensively studied by scientists, looking for an ideal transmission-blocking vaccine target. IGEM EPFL team's interest goes toward the ookinete surface membrane proteins P25 and P28. In fact, it has been shown that the binding of specific antibodies to these surface proteins was fatal to more than 99% of the parasites reference. Indeed, P25 and P28 seem to be essential to the ookinete survival, the crossing of the midgut epithelium and its transformation into an oocyst. The team decided to design Asaia to express a soluble form of these 2 P-proteins. It could seems contra-intuitive to provide more of these proteins (by expression in Asaia) that are very important for the parasite survival, migration, and development. But our hypothesis is that adding a lot of these proteins in a soluble form will compete with the parasite surface protein and prevent the interaction necessary to its transmission. Because the functions of P25 and P28 are redundant, both proteins need to be inhibited or outcompeted to efficiently block the malaria transmission. false false _439_ 0 7149 9 Not in stock false The coding for the P protein was AT to richa nd had to be optimized for Asaia and E.Coli. false Perrudet Christian annotation2095168 1 BBa_K320009 range2095168 1 766 1533 annotation2095169 1 BBa_K320003 range2095169 1 1534 4223 annotation2095167 1 BBa_K320007 range2095167 1 1 765 BBa_K320014_sequence 1 ctagagaatacttgacatatcactgtgattcacatataatatgcgcatcctcttgataattgctagccttaagaaggaggaatactagatgaataaactttacagtttgtttcttttccttttcattcaacttagcataaaatataataatgcgaaagttaccgtggatactgtatgcaaaagaggatttttaattcagatgagtggtcatttggaatgtaaatgtgaaaatgatttggtgttagtaaatgaagaaacatgtgaagaaaaagttctgaaatgtgacgaaaagactgtaaataaaccatgtggagatttttccaaatgtattaaaatagatggaaatcccgtttcatacgcttgtaaatgtaatcttggatatgatatggtaaataatgtttgtataccaaatgaatgtaagaatgtaacttgtggtaacggtaaatgtatattagatacaagcaatcctgttaaaactggagtttgctcatgtaatataggcaaagttcccaatgtacaagatcaaaataaatgttcaaaagatggagaaaccaaatgctcattaaaatgcttaaaagaaaatgaaacctgtaaagctgttgatggaatttataaatgtgattgtaaagatggatttataatagataatgaaagctctatatgtactgctttttcagcatataatattttaaatctaagcattatgtttatactattttcagtatgcttttttataatgcatcatcatcatcatcatcactaatactagagaatacttgacatatcactgtgattcacatataatatgcgcatcctcttgataattgctagccttaagaaggaggaatactagatgaatacatattttaaggtacttctttttttatttattcaactttacataacgttgaataaggctcgggttactgaaaatacaatatgtaaatatggttatttaattcagatgagtaatcattatgaatgtaagtgtattgaaggatatgtattaataaatgaggacacgtgtggaaaaaaagtagtctgtgataaagttgaaaattcatttaaagcttgtgatgaatacgcttactgtttcgatttaggaaataagaataatgaaaaacagataaaatgtatgtgcagaacagaatatactttaactgctggagtatgtgttcctaatgtttgtcgagataaagtatgtggtaaaggaaaatgtatagtagatcctgcaaattctttaacacatacatgctcatgcaatataggtaccatacttaaccagaataaattatgtgatatacaaggtgatacaccatgttcattaaaatgtgcagaaaatgaagtgtgtacattagaaggaaattattatacatgtaaagaagatccttcatctaacggaggaggaaatactgtggaccaggctgatacatcatatagtgtaataaacggagtaaccctaacacacgttctgattgtatgctcaatatttattaaattgttaatacatcatcatcatcatcatcactaatactagagaccttgggcaaggccgaaggccgcgcagtcgatcaacaagccccggaggggccactttttgccggagggggagccgcgccgaaggcgtgggggaaccccgcaggggtgcccttctttgggcaccaaagaactagatatagggcgaaatgcgaaagacttaaaaatcaacaacttaaaaaaggggggtacgcaacagctcattgcggcaccccccgcaatagctcattgcgtaggttaaagaaaatctgtaattgactgccacttttacgcaacgcataattgttgtcgcgctgccgaaaagttgcagctgattgcgcatggtgccgcaaccgtgcggcaccctaccgcatggagataagcatggccacgcagtccagagaaatcggcattcaagccaagaacaagcccggtcactgggtgcaaacggaacgcaaagcgcatgaggcgtgggccgggcttattgcgaggaaacccacggcggcaatgctgctgcatcacctcgtggcgcagatgggccaccagaacgccgtggtggtcagccagaagacactttccaagctcatcggacgttctttgcggacggtccaatacgcagtcaaggacttggtggccgagcgctggatctccgtcgtgaagctcaacggccccggcaccgtgtcggcctacgtggtcaatgaccgcgtggcgtggggccagccccgcgaccagttgcgcctgtcggtgttcagtgccgccgtggtggttgatcacgacgaccaggacgaatcgctgttggggcatggcgacctgcgccgcatcccgaccctgtatccgggcgagcagcaactaccgaccggccccggcgaggagccgcccagccagcccggcattccgggcatggaaccagacctgccagccttgaccgaaacggaggaatgggaacggcgcgggcagcagcgcctgccgatgcccgatgagccgtgttttctggacgatggcgagccgttggagccgccgacacgggtcacgctgccgcgccggtagcacttgggttgcgcagcaacccgtaagtgcgctgttccagactatcggctgtagccgcctcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccgtttttatcaggctctgggaggcagaataaatgatcatatcgtcaattattacctccacggggagagcctgagcaaactggcctcaggcatttgagaagcacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgaccgggtcgaattttactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z