BBa_K322128
type: ComponentDefinition
persistent identity: BBa K322128
display id: BBa_K322128
version: 1
was generated by: iGEM to SBOL conversion
description: Phycocyanobilin synthesis genes + Red light detection system (EYFP as reporter)
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: PCR of BBa_M30109 (which is truncated in the 2010 igem distribution).
owned by: james
owned by: myers
mutable description: There are some problems with the original coliroid parts that are in the 2010 distribution. We resubmitted this in pSB1C3, with the reporter system attached. This part contains the phycocyanobilin synthesis genes. PCB binds to cph8 and is required for light detection.
discontinued: false
dominant: false
experience: experience/None
group u list: _441_
m user id: 0
owner id: 7053
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: EYFP is used as a reporter system for precise characterisation.
star: false
creator: Chris French and Richard Partridge Hicks
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J33202
component: BBa_R0082
component: BBa_E0030
component: BBa_K322124
component: BBa_K322123
sequence annotation: BBa_K322123
sequence annotation: BBa_K322124
sequence annotation: BBa_R0082
sequence annotation: BBa_J33202
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_K322128_sequence/1

BBa_J33202
type: Component
persistent identity: component2084195
display id: component2084195
version: 1
top level: BBa_K322128
definition: BBa_J33202
access: public

BBa_R0082
type: Component
persistent identity: component2084192
display id: component2084192
version: 1
top level: BBa_K322128
definition: OmpR
access: public

BBa_E0030
type: Component
persistent identity: component2084202
display id: component2084202
version: 1
top level: BBa_K322128
definition: eyfp
access: public

BBa_R0082
type: Component
persistent identity: component2084201
display id: component2084201
version: 1
top level: BBa_K322128
definition: OmpR
access: public

BBa_K322124
type: Component
persistent identity: component2084186
display id: component2084186
version: 1
top level: BBa_K322128
definition: BBa_K322124
access: public

BBa_K322123
type: Component
persistent identity: component2084183
display id: component2084183
version: 1
top level: BBa_K322128
definition: BBa_K322123
access: public

BBa_K322123
type: SequenceAnnotation
persistent identity: annotation2084183
display id: annotation2084183
version: 1
top level: BBa_K322128
location: https://synbiohub.org/public/igem/BBa_K322128/annotation2084183/range2084183/1
component: BBa_K322123

https://synbiohub.org/public/igem/BBa_K322128/annotation2084183/range2084183/1
type: Range
persistent identity: range2084183
display id: range2084183
version: 1
top level: BBa_K322128
direction: direction/reverse
start: 1
end: 1399
orientation: inline

BBa_K322124
type: SequenceAnnotation
persistent identity: annotation2084186
display id: annotation2084186
version: 1
top level: BBa_K322128
location: https://synbiohub.org/public/igem/BBa_K322128/annotation2084186/range2084186/1
component: BBa_K322124

https://synbiohub.org/public/igem/BBa_K322128/annotation2084186/range2084186/1
type: Range
persistent identity: range2084186
display id: range2084186
version: 1
top level: BBa_K322128
direction: direction/reverse
start: 1408
end: 3660
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2084192
display id: annotation2084192
version: 1
top level: BBa_K322128
location: https://synbiohub.org/public/igem/BBa_K322128/annotation2084192/range2084192/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K322128/annotation2084192/range2084192/1
type: Range
persistent identity: range2084192
display id: range2084192
version: 1
top level: BBa_K322128
direction: direction/reverse
start: 3669
end: 3776
orientation: inline

BBa_J33202
type: SequenceAnnotation
persistent identity: annotation2084195
display id: annotation2084195
version: 1
top level: BBa_K322128
location: https://synbiohub.org/public/igem/BBa_K322128/annotation2084195/range2084195/1
component: BBa_J33202

https://synbiohub.org/public/igem/BBa_K322128/annotation2084195/range2084195/1
type: Range
persistent identity: range2084195
display id: range2084195
version: 1
top level: BBa_K322128
direction: direction/reverse
start: 3785
end: 4027
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2084201
display id: annotation2084201
version: 1
top level: BBa_K322128
location: https://synbiohub.org/public/igem/BBa_K322128/annotation2084201/range2084201/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K322128/annotation2084201/range2084201/1
type: Range
persistent identity: range2084201
display id: range2084201
version: 1
top level: BBa_K322128
direction: direction/reverse
start: 4036
end: 4143
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2084202
display id: annotation2084202
version: 1
top level: BBa_K322128
location: https://synbiohub.org/public/igem/BBa_K322128/annotation2084202/range2084202/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K322128/annotation2084202/range2084202/1
type: Range
persistent identity: range2084202
display id: range2084202
version: 1
top level: BBa_K322128
direction: direction/reverse
start: 4150
end: 4872
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

BBa_K322123
type: ComponentDefinition
persistent identity: BBa K322123
display id: BBa_K322123
version: 1
was generated by: iGEM to SBOL conversion
description: Phycobilin synthesis operon without terminator
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: This is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon.
owned by: james
owned by: myers
mutable description: Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor.
discontinued: false
dominant: false
experience: experience/None
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The actual forward sequence has the same changes at the start as seen in clone H11 described above. The reverse sequence is as expected. Features same as for the one above apart from the terminator.
star: false
creator: Chris French and Richard Edward Partridge-Hicks
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: J23110
sequence annotation: B0032
sequence annotation: Heme oxygenase
sequence annotation: rbs
sequence annotation: pcyA
sequence: https://synbiohub.org/public/igem/BBa_K322123_sequence/1

J23110
type: SequenceAnnotation
persistent identity: annotation2083043
display id: annotation2083043
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083043/range2083043/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K322123/annotation2083043/range2083043/1
type: Range
persistent identity: range2083043
display id: range2083043
version: 1
top level: BBa_K322123
direction: direction/forward
start: 1
end: 35
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2083044
display id: annotation2083044
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083044/range2083044/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K322123/annotation2083044/range2083044/1
type: Range
persistent identity: range2083044
display id: range2083044
version: 1
top level: BBa_K322123
direction: direction/forward
start: 43
end: 54
orientation: inline

Heme oxygenase
type: SequenceAnnotation
persistent identity: annotation2083045
display id: annotation2083045
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083045/range2083045/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K322123/annotation2083045/range2083045/1
type: Range
persistent identity: range2083045
display id: range2083045
version: 1
top level: BBa_K322123
direction: direction/forward
start: 63
end: 629
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2083046
display id: annotation2083046
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083046/range2083046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K322123/annotation2083046/range2083046/1
type: Range
persistent identity: range2083046
display id: range2083046
version: 1
top level: BBa_K322123
direction: direction/forward
start: 630
end: 644
orientation: inline

pcyA
type: SequenceAnnotation
persistent identity: annotation2083047
display id: annotation2083047
version: 1
top level: BBa_K322123
location: https://synbiohub.org/public/igem/BBa_K322123/annotation2083047/range2083047/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K322123/annotation2083047/range2083047/1
type: Range
persistent identity: range2083047
display id: range2083047
version: 1
top level: BBa_K322123
direction: direction/forward
start: 650
end: 1396
orientation: inline

BBa_K322124
type: ComponentDefinition
persistent identity: BBa K322124
display id: BBa_K322124
version: 1
was generated by: iGEM to SBOL conversion
description: Red light sensor Cph8 (from Coliroid) with RBS
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:59Z
mutable provenance: BBa_I15010 (Photosensing Chimaera (Cph8) (not working, but amplified out of the whole construct))
owned by: james
owned by: myers
mutable description: Cph8 is a combination of cph1 and envZ which, together with PCB, form a transmembrane, histidine kinase-based light sensing system. It contains the photoreceptor from Cph1 and the histidine kinase and response-regulator from EnvZ???OmpR, producing a particularly strongresponse to light. Features: cph8 coding sequence 16 to 2250, RBS 6 to 10.
discontinued: false
dominant: false
experience: experience/None
group u list: _441_
m user id: 0
owner id: 6225
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Notes: we were unable to revive BioBrick I15010 with the coding sequence of the cph8 red light sensor fusion protein, so we sought to amplify it from BioBrick BBa_M30109. This led to numerous problems. It is clear from the Registry sequence data that M30109 is not what it is supposed to be. It does include cph8 with a ribosome binding site, but the RBS is not joined to cph8 by a standard scar; promoter R0040 may be present, but there appears to be an EcoRI site a little upstream of the ribosome binding site so that, when we cloned our PCR product, we failed to recover the promoter region; also the PstI site in cph8, which was mutated out in I15010, is still present in the version in M30109. We therefore performed corrective PCR to obtain a clone with the correct prefix, a ribosome binding site, and the cph8 coding sequence with the PstI site properly removed, which we present here, cloned in pSB1C3.
star: false
creator: Chris French and Richard Edward Partridge-Hicks
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: Cph8
sequence: https://synbiohub.org/public/igem/BBa_K322124_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2083481
display id: annotation2083481
version: 1
top level: BBa_K322124
location: https://synbiohub.org/public/igem/BBa_K322124/annotation2083481/range2083481/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K322124/annotation2083481/range2083481/1
type: Range
persistent identity: range2083481
display id: range2083481
version: 1
top level: BBa_K322124
direction: direction/forward
start: 6
end: 10
orientation: inline

Cph8
type: SequenceAnnotation
persistent identity: annotation2083482
display id: annotation2083482
version: 1
top level: BBa_K322124
location: https://synbiohub.org/public/igem/BBa_K322124/annotation2083482/range2083482/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K322124/annotation2083482/range2083482/1
type: Range
persistent identity: range2083482
display id: range2083482
version: 1
top level: BBa_K322124
direction: direction/forward
start: 16
end: 2250
orientation: inline

BBa_J33202
type: ComponentDefinition
persistent identity: BBa J33202
display id: BBa_J33202
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ
created: 2006-10-12T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: The DNA was derived by PCR from E. coli BL21, which possesses an intact lacZ gene. Primers were designed based on sequence from GenBank accession J01636, GI:146575. A stop codon was artificially introduced to replace codon 78. The sequence reported here is derived by sequencing of the Biobrick construct.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This gene encodes the N-terminal 77 amino acid residues of LacZ. When expressed in an E. coli host carrying the lacZ-delta-M15 mutation, common in laboratory strains, it complements the delection resulting in the production of active LacZ, which can be detected by various means, including chromogenic substrates such as Xgal (5-bromo-4-chloro-3-indolyl-beta-D-galactoside) and ONPG (o-nitrophenyl galactoside).
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Note that a stop codon was introduced to replace codon 78 of lacZ, resulting in production only of the N-terminal 77 amino acid residues of LacZ. This is sufficient to complement the lacZ-delta-M15 mutation commonly found in laboratory strains of E. col used for alpha-complementation.
star: false
creator: Chris French
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: lacZ'
sequence: https://synbiohub.org/public/igem/BBa_J33202_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1902818
display id: annotation1902818
version: 1
top level: BBa_J33202
location: https://synbiohub.org/public/igem/BBa_J33202/annotation1902818/range1902818/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J33202/annotation1902818/range1902818/1
type: Range
persistent identity: range1902818
display id: range1902818
version: 1
top level: BBa_J33202
direction: direction/forward
start: 1
end: 4
orientation: inline

lacZ'
type: SequenceAnnotation
persistent identity: annotation1902819
display id: annotation1902819
version: 1
top level: BBa_J33202
location: https://synbiohub.org/public/igem/BBa_J33202/annotation1902819/range1902819/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J33202/annotation1902819/range1902819/1
type: Range
persistent identity: range1902819
display id: range1902819
version: 1
top level: BBa_J33202
direction: direction/forward
start: 13
end: 243
orientation: inline

https://synbiohub.org/public/igem/BBa_K322128_sequence/1
type: Sequence
persistent identity: BBa K322128 sequence
display id: BBa_K322128_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataatactagagaggagaaaggtacccatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacggga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K322123_sequence/1
type: Sequence
persistent identity: BBa K322123 sequence
display id: BBa_K322123_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtattatgctagctactagagtcacacaggaaaggtgcacatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctagttaaagaggagaaaggatccatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K322124_sequence/1
type: Sequence
persistent identity: BBa K322124 sequence
display id: BBa_K322124_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggagaaaggtacccatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J33202_sequence/1
type: Sequence
persistent identity: BBa J33202 sequence
display id: BBa_J33202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z