LOCUS       BBa_K322140             2718 bp    DNA     linear       22-Dec-2024
DEFINITION  lac promoter + luxAB
ACCESSION   BBa_K322140
VERSION     BBa_K322140.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..2718
                     /label=BBa_K322139
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaatagcaa tataaggact ctctatgaaa tttggaaact ttttgcttac
      661 ataccaacct ccccaatttt ctcaaacaga ggtaatgaaa cgtttggtta aattaggtcg
      721 catctctgag gagtgtggtt ttgataccgt atggttactg gagcatcatt tcacggagtt
      781 tggtttgctt ggtaaccctt atgtcgctgc tgcatattta cttggcgcga ctaaaaaatt
      841 gaatgtagga actgccgcta ttgttcttcc cacagcccat ccagtacgcc aacttgaaga
      901 tgtgaattta ttggatcaaa tgtcaaaagg acgatttcgg tttggtattt gccgagggct
      961 ttacaacaag gactttcgcg tattcggcac agatatgaat aacagtcgcg ccttagcgga
     1021 atgctggtac gggctgataa agaatggcat gacagaggga tatatggaag ctgataatga
     1081 acatatcaag ttccataagg taaaagtaaa ccccgcggcg tatagcagag gtggcgcacc
     1141 ggtttatgtg gtggctgaat cagcttcgac gactgagtgg gctgctcaat ttggcctacc
     1201 gatgatatta agttggatta taaatactaa cgaaaagaaa gcacaacttg agctttataa
     1261 tgaagtggct caagaatatg ggcacgatat tcataatatc gaccattgct tatcatatat
     1321 aacatctgta gatcatgact caattaaagc gaaagagatt tgccggaaat ttctggggca
     1381 ttggtatgat tcttatgtga atgctacgac tatttttgat gattcagacc aaacaagagg
     1441 ttatgatttc aataaagggc agtggcgtga ctttgtatta aaaggacata aagatactaa
     1501 tcgccgtatt gattacagtt acgaaatcaa tcccgtggga acgccgcagg aatgtattga
     1561 cataattcaa aaagacattg atgctacagg aatatcaaat atttgttgtg gatttgaagc
     1621 taatggaaca gtagacgaaa ttattgcttc catgaagctc ttccagtctg atgtcatgcc
     1681 atttcttaaa gaaaaacaac gttcgctatt atattagcta aggagaaaga aatgaaattt
     1741 ggattgttct tccttaactt catcaattca acaactgttc aagaacaaag tatagttcgc
     1801 atgcaggaaa taacggagta tgttgataag ttgaattttg aacagatttt agtgtatgaa
     1861 aatcattttt cagataatgg tgttgtcggc gctcctctga ctgtttctgg ttttctgctc
     1921 ggtttaacag agaaaattaa aattggttca ttaaatcaca tcattacaac tcatcatcct
     1981 gtcgccatag cggaggaagc ttgcttattg gatcagttaa gtgaagggag atttatttta
     2041 gggtttagtg attgcgaaaa aaaagatgaa atgcattttt ttaatcgccc ggttgaatat
     2101 caacagcaac tatttgaaga gtgttatgaa atcattaacg atgctttaac aacaggctat
     2161 tgtaatccag ataacgattt ttatagcttc cctaaaatat ctgtaaatcc ccatgcttat
     2221 acgccaggcg gacctcggaa atatgtaaca gcaaccagtc atcatattgt tgagtgggcg
     2281 gccaaaaaag gtattcctct catctttaag tgggatgatt ctaatgatgt tagatatgaa
     2341 tatgctgaaa gatataaagc cgttgcggat aaatatgacg ttgacctatc agagatagac
     2401 catcagttaa tgatattagt taactataac gaagatagta ataaagctaa acaagagacg
     2461 cgtgcattta ttagtgatta tgttcttgaa atgcacccta atgaaaattt cgaaaataaa
     2521 cttgaagaaa taattgcaga aaacgctgtc ggaaattata cggagtgtat aactgcggct
     2581 aagttggcaa ttgaaaagtg tggtgcgaaa agtgtattgc tgtcctttga accaatgaat
     2641 gatttgatga gccaaaaaaa tgtaatcaat attgttgatg ataatattaa gaagtaccac
     2701 atggaatata cctaataa
//