BBa_K322139 1 BBa_K322139 Blue emitting luciferase (from X. luminescens) 2010-09-24T11:00:00Z 2015-05-08T01:11:59Z Source: genomic DNA of Xenorhabdus (Photorhabdus) luminescens Hb (actually, we used our BioBrick from last year, BBa_K216016 as template). false false _441_ 0 6225 9 In stock true Notes: we prepared this for two reasons: firstly to resolve an issue with the SpeI site in our submitted version of K216008 (for some reason, every clone we sequenced seemed to have an error, not always the same error, in the suffix SpeI site), and to put it in pSB1C3, since this seems to be the new standard vector for the Registry. false Richard Partridge-Hicks and Maria Kowal BBa_K322007 1 BBa_K322007 Lumazine protein (LumP). Causes blue shift of <i>P. phosphoreum</i> luciferase emmision spectrum. 2010-09-25T11:00:00Z 2015-05-08T01:11:59Z Source: genomic DNA of Photobacterium phosphoreum. This is the lumazine protein of Photobacterium phosphoreum. Lumazine protein (LumP) of Photobacterium phosphoreum is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In P. phosphoreum, it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein). false false _441_ 0 6225 9 It's complicated true We submitted a version of lumP last year (BBa_K216007), but made a new one since we were having some issues working with our clone (lots of red colonies). So we made a new one in pSB1C3 starting from the original PCR product. On sequencing this (as well as looking at the sequence results from the original plasmids, M1117 and M1118) it was found that the sequence differed from the one we had submitted in a number of mutations, some silent, some not. false Chris French and Maria Kowal annotation2083463 1 lumP range2083463 1 10 578 annotation2083462 1 rbs range2083462 1 1 9 BBa_K322149 1 BBa_K322149 Bacterial luciferase (luxAB) blue shifted by lumazine protein 2010-10-07T11:00:00Z 2015-05-08T01:11:59Z See individual biobricks. The spectral output of the bacterial luciferase from X. luminescens is known to shift towards blue wavelengths in presence of lumazine protein (lumP). This part is made of both these biobricks. false false _441_ 0 7053 9 It's complicated true LuxAB uses decanal as a substrate. This can either be added to the medium, or can be synthesised by providing lucCDE (BBa_K322312). false Chris French and Richard Partridge Hicks component2084070 1 BBa_K322139 component2084073 1 BBa_K322007 annotation2084073 1 BBa_K322007 range2084073 1 2119 2700 annotation2084070 1 BBa_K322139 range2084070 1 1 2110 BBa_K322139_sequence 1 ctcaaatagcaatataaggactctctatgaaatttggaaactttttgcttacataccaacctccccaattttctcaaacagaggtaatgaaacgtttggttaaattaggtcgcatctctgaggagtgtggttttgataccgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggcgcgactaaaaaattgaatgtaggaactgccgctattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgagggctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcgcgccttagcggaatgctggtacgggctgataaagaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaaccccgcggcgtatagcagaggtggcgcaccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctcaatttggcctaccgatgatattaagttggattataaatactaacgaaaagaaagcacaacttgagctttataatgaagtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtagatcatgactcaattaaagcgaaagagatttgccggaaatttctggggcattggtatgattcttatgtgaatgctacgactatttttgatgattcagaccaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgtattgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtattgacataattcaaaaagacattgatgctacaggaatatcaaatatttgttgtggatttgaagctaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgctattatattagctaaggagaaagaaatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttagtgtatgaaaatcatttttcagataatggtgttgtcggcgctcctctgactgtttctggttttctgctcggtttaacagagaaaattaaaattggttcattaaatcacatcattacaactcatcatcctgtcgccatagcggaggaagcttgcttattggatcagttaagtgaagggagatttattttagggtttagtgattgcgaaaaaaaagatgaaatgcatttttttaatcgcccggttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatccagataacgatttttatagcttccctaaaatatctgtaaatccccatgcttatacgccaggcggacctcggaaatatgtaacagcaaccagtcatcatattgttgagtgggcggccaaaaaaggtattcctctcatctttaagtgggatgattctaatgatgttagatatgaatatgctgaaagatataaagccgttgcggataaatatgacgttgacctatcagagatagaccatcagttaatgatattagttaactataacgaagatagtaataaagctaaacaagagacgcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaatttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaattatacggagtgtataactgcggctaagttggcaattgaaaagtgtggtgcgaaaagtgtattgctgtcctttgaaccaatgaatgatttgatgagccaaaaaaatgtaatcaatattgttgatgataatattaagaagtaccacatggaatatacctaataa BBa_K322007_sequence 1 gagattattatgttcaaaggtatagttcagggcactggaataattaaaaaaatatcaaaaaatgatgatacccaaagacatggcattacttttccaaaaaatatattggacttagttgcaaaagatactgtcatgctcgtaaatggatgctcattaactgttgttcgcatcacaggtgatgtcgtttatttcgatatagatcaggcacttaataccacaacctttgattcattagaaatcggtgacaaggtaaacttagaactacgtccagaatttggctcactccttgggaaaggtgctttaactggaaatataaagggggtagctactgttgataatattactgaagaagaagatcttcttaaagtttatattgaaattcctaaaaacctaaccaaaaatatttccgcagaagatcatattggaattaatggtgtatctaattctattgaagaagtttccaatgatattatttgcattaattatcccaaaaatttatctattaccaccaaccttagcactttagaagcaggttcgaaggttaacgtagaaacattaaatgtatcaaatgaatggtaataa BBa_K322149_sequence 1 ctcaaatagcaatataaggactctctatgaaatttggaaactttttgcttacataccaacctccccaattttctcaaacagaggtaatgaaacgtttggttaaattaggtcgcatctctgaggagtgtggttttgataccgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggcgcgactaaaaaattgaatgtaggaactgccgctattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgagggctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcgcgccttagcggaatgctggtacgggctgataaagaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaaccccgcggcgtatagcagaggtggcgcaccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctcaatttggcctaccgatgatattaagttggattataaatactaacgaaaagaaagcacaacttgagctttataatgaagtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtagatcatgactcaattaaagcgaaagagatttgccggaaatttctggggcattggtatgattcttatgtgaatgctacgactatttttgatgattcagaccaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgtattgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtattgacataattcaaaaagacattgatgctacaggaatatcaaatatttgttgtggatttgaagctaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgctattatattagctaaggagaaagaaatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttagtgtatgaaaatcatttttcagataatggtgttgtcggcgctcctctgactgtttctggttttctgctcggtttaacagagaaaattaaaattggttcattaaatcacatcattacaactcatcatcctgtcgccatagcggaggaagcttgcttattggatcagttaagtgaagggagatttattttagggtttagtgattgcgaaaaaaaagatgaaatgcatttttttaatcgcccggttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatccagataacgatttttatagcttccctaaaatatctgtaaatccccatgcttatacgccaggcggacctcggaaatatgtaacagcaaccagtcatcatattgttgagtgggcggccaaaaaaggtattcctctcatctttaagtgggatgattctaatgatgttagatatgaatatgctgaaagatataaagccgttgcggataaatatgacgttgacctatcagagatagaccatcagttaatgatattagttaactataacgaagatagtaataaagctaaacaagagacgcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaatttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaattatacggagtgtataactgcggctaagttggcaattgaaaagtgtggtgcgaaaagtgtattgctgtcctttgaaccaatgaatgatttgatgagccaaaaaaatgtaatcaatattgttgatgataatattaagaagtaccacatggaatatacctaataatactagaggagattattatgttcaaaggtatagttcagggcactggaataattaaaaaaatatcaaaaaatgatgatacccaaagacatggcattacttttccaaaaaatatattggacttagttgcaaaagatactgtcatgctcgtaaatggatgctcattaactgttgttcgcatcacaggtgatgtcgtttatttcgatatagatcaggcacttaataccacaacctttgattcattagaaatcggtgacaaggtaaacttagaactacgtccagaatttggctcactccttgggaaaggtgctttaactggaaatataaagggggtagctactgttgataatattactgaagaagaagatcttcttaaagtttatattgaaattcctaaaaacctaaccaaaaatatttccgcagaagatcatattggaattaatggtgtatctaattctattgaagaagtttccaatgatattatttgcattaattatcccaaaaatttatctattaccaccaaccttagcactttagaagcaggttcgaaggttaacgtagaaacattaaatgtatcaaatgaatggtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z