BBa_K322139
1
BBa_K322139
Blue emitting luciferase (from X. luminescens)
2010-09-24T11:00:00Z
2015-05-08T01:11:59Z
Source: genomic DNA of Xenorhabdus (Photorhabdus) luminescens Hb (actually, we used our BioBrick from last year, BBa_K216016 as template).
false
false
_441_
0
6225
9
In stock
true
Notes: we prepared this for two reasons: firstly to resolve an issue with the SpeI site in our submitted version of K216008 (for some reason, every clone we sequenced seemed to have an error, not always the same error, in the suffix SpeI site), and to put it in pSB1C3, since this seems to be the new standard vector for the Registry.
false
Richard Partridge-Hicks and Maria Kowal
BBa_K322149
1
BBa_K322149
Bacterial luciferase (luxAB) blue shifted by lumazine protein
2010-10-07T11:00:00Z
2015-05-08T01:11:59Z
See individual biobricks.
The spectral output of the bacterial luciferase from X. luminescens is known to shift towards blue wavelengths in presence of lumazine protein (lumP). This part is made of both these biobricks.
false
false
_441_
0
7053
9
It's complicated
true
LuxAB uses decanal as a substrate. This can either be added to the medium, or can be synthesised by providing lucCDE (BBa_K322312).
false
Chris French and Richard Partridge Hicks
component2084073
1
BBa_K322007
component2084070
1
BBa_K322139
annotation2084070
1
BBa_K322139
range2084070
1
1
2110
annotation2084073
1
BBa_K322007
range2084073
1
2119
2700
BBa_K322007
1
BBa_K322007
Lumazine protein (LumP). Causes blue shift of <i>P. phosphoreum</i> luciferase emmision spectrum.
2010-09-25T11:00:00Z
2015-05-08T01:11:59Z
Source: genomic DNA of Photobacterium phosphoreum.
This is the lumazine protein of Photobacterium phosphoreum. Lumazine protein (LumP) of Photobacterium phosphoreum is a small protein which binds a fluorescent pteridine, 6,7-dimethyl-8-(1'-D-ribityl)lumazine, as cofactor. In P. phosphoreum, it acts to shift the wavelength of light emitted by luciferase, LuxAB, from 495 nm to a shorter wavelength, around 475 nm (O'Kane et al, 1990 and references cited therein).
false
false
_441_
0
6225
9
It's complicated
true
We submitted a version of lumP last year (BBa_K216007), but made a new one since we were having some issues working with our clone (lots of red colonies). So we made a new one in pSB1C3 starting from the original PCR product. On sequencing this (as well as looking at the sequence results from the original plasmids, M1117 and M1118) it was found that the sequence differed from the one we had submitted in a number of mutations, some silent, some not.
false
Chris French and Maria Kowal
annotation2083462
1
rbs
range2083462
1
1
9
annotation2083463
1
lumP
range2083463
1
10
578
BBa_K322150
1
BBa_K322150
Bacterial luciferase (luxAB) blue shifted by lumazine protein, with promoter
2010-10-07T11:00:00Z
2015-05-08T01:11:59Z
See individual parts
Bacterial luciferase from X. luminescens (luxAB) with Lumazine protein which shifts its spectrum towards blue. Note that LuxAB uses decanal as a substrate, this needs to be added or synthesised by the bacteria (Decanal production is coded for by BBa_K322312).
false
false
_441_
0
7053
9
It's complicated
true
Decanal must be added or synthesised by LuxCDE (see Ba_K322312).
Note that this part also contains a LacZ fragment between the promoter and luxAB.
false
Chris French and Richard Partridge Hicks
component2099142
1
BBa_J33207
component2099147
1
BBa_K322149
annotation2099147
1
BBa_K322149
range2099147
1
609
3308
annotation2099142
1
BBa_J33207
range2099142
1
1
600
BBa_J33207
1
BBa_J33207
lac promoter and lacZ
2006-10-26T11:00:00Z
2015-08-31T04:08:46Z
The DNA was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). The annotation shown here is based on that associated with this Genbank entry. The sequence shown here is derived by sequencing the construct.
This part (submitted in pSB1A2) consists of the lac promoter and lacZ' gene, encoding the N-terminal 76 amino acid residues of LacZ, sufficient to complement the lacZ-delta-M15 mutation for blue-white selection on Xgal plates. A SacI site has been introduced at the 3' end, overlapping the XbaI site of the Biobrick prefix. This is designed to be used as a cloning vector for making new biobricks. PCR primers can be designed with a SacI site in one primer and an SpeI site in the other. This removes the necessity for an excessively long non-complementary tail on one primer bearing either the full biobrick prefix or suffix. The PCR product can then be digested with SacI and SpeI for insertion into this plasmid, replacing the Plac-lacZ' cassette. Recombinant plasmids will then be white on IPTG/Xgal plates, whereas any that still contain the original insert will be blue. We have used this strategy to prepare several biobricks, including BBa_J33204, which contains the xylE gene encoding catechol-2,3-dioxygenase. (For making biobricks that contain lacZ', BBa_J33204 can be used in the same way; in this case, clones with plasmids that still contain xylE will turn yellow on addition of a drop of 10 mM catechol.)
false
false
_63_
0
837
63
It's complicated
true
Note that the SacI site overlaps the SpeI site. The Biobrick prefix ends ...TCTAGAG. When this is added to the CTC at the start of the sequence shown here, the SacI site, GAGCTC, is generated.
false
Chris French
annotation1907857
1
rbs
range1907857
1
359
362
annotation1907858
1
lacZ'
range1907858
1
370
600
annotation1907854
1
SacI
range1907854
1
1
3
annotation1907855
1
CAP binding site
range1907855
1
248
285
annotation1907856
1
LacI binding site
range1907856
1
332
352
annotation1907859
1
-10
range1907859
1
320
325
annotation1907860
1
-35
range1907860
1
297
302
BBa_K322150_sequence
1
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagagctcaaatagcaatataaggactctctatgaaatttggaaactttttgcttacataccaacctccccaattttctcaaacagaggtaatgaaacgtttggttaaattaggtcgcatctctgaggagtgtggttttgataccgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggcgcgactaaaaaattgaatgtaggaactgccgctattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgagggctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcgcgccttagcggaatgctggtacgggctgataaagaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaaccccgcggcgtatagcagaggtggcgcaccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctcaatttggcctaccgatgatattaagttggattataaatactaacgaaaagaaagcacaacttgagctttataatgaagtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtagatcatgactcaattaaagcgaaagagatttgccggaaatttctggggcattggtatgattcttatgtgaatgctacgactatttttgatgattcagaccaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgtattgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtattgacataattcaaaaagacattgatgctacaggaatatcaaatatttgttgtggatttgaagctaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgctattatattagctaaggagaaagaaatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttagtgtatgaaaatcatttttcagataatggtgttgtcggcgctcctctgactgtttctggttttctgctcggtttaacagagaaaattaaaattggttcattaaatcacatcattacaactcatcatcctgtcgccatagcggaggaagcttgcttattggatcagttaagtgaagggagatttattttagggtttagtgattgcgaaaaaaaagatgaaatgcatttttttaatcgcccggttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatccagataacgatttttatagcttccctaaaatatctgtaaatccccatgcttatacgccaggcggacctcggaaatatgtaacagcaaccagtcatcatattgttgagtgggcggccaaaaaaggtattcctctcatctttaagtgggatgattctaatgatgttagatatgaatatgctgaaagatataaagccgttgcggataaatatgacgttgacctatcagagatagaccatcagttaatgatattagttaactataacgaagatagtaataaagctaaacaagagacgcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaatttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaattatacggagtgtataactgcggctaagttggcaattgaaaagtgtggtgcgaaaagtgtattgctgtcctttgaaccaatgaatgatttgatgagccaaaaaaatgtaatcaatattgttgatgataatattaagaagtaccacatggaatatacctaataatactagaggagattattatgttcaaaggtatagttcagggcactggaataattaaaaaaatatcaaaaaatgatgatacccaaagacatggcattacttttccaaaaaatatattggacttagttgcaaaagatactgtcatgctcgtaaatggatgctcattaactgttgttcgcatcacaggtgatgtcgtttatttcgatatagatcaggcacttaataccacaacctttgattcattagaaatcggtgacaaggtaaacttagaactacgtccagaatttggctcactccttgggaaaggtgctttaactggaaatataaagggggtagctactgttgataatattactgaagaagaagatcttcttaaagtttatattgaaattcctaaaaacctaaccaaaaatatttccgcagaagatcatattggaattaatggtgtatctaattctattgaagaagtttccaatgatattatttgcattaattatcccaaaaatttatctattaccaccaaccttagcactttagaagcaggttcgaaggttaacgtagaaacattaaatgtatcaaatgaatggtaataa
BBa_K322139_sequence
1
ctcaaatagcaatataaggactctctatgaaatttggaaactttttgcttacataccaacctccccaattttctcaaacagaggtaatgaaacgtttggttaaattaggtcgcatctctgaggagtgtggttttgataccgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggcgcgactaaaaaattgaatgtaggaactgccgctattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgagggctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcgcgccttagcggaatgctggtacgggctgataaagaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaaccccgcggcgtatagcagaggtggcgcaccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctcaatttggcctaccgatgatattaagttggattataaatactaacgaaaagaaagcacaacttgagctttataatgaagtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtagatcatgactcaattaaagcgaaagagatttgccggaaatttctggggcattggtatgattcttatgtgaatgctacgactatttttgatgattcagaccaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgtattgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtattgacataattcaaaaagacattgatgctacaggaatatcaaatatttgttgtggatttgaagctaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgctattatattagctaaggagaaagaaatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttagtgtatgaaaatcatttttcagataatggtgttgtcggcgctcctctgactgtttctggttttctgctcggtttaacagagaaaattaaaattggttcattaaatcacatcattacaactcatcatcctgtcgccatagcggaggaagcttgcttattggatcagttaagtgaagggagatttattttagggtttagtgattgcgaaaaaaaagatgaaatgcatttttttaatcgcccggttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatccagataacgatttttatagcttccctaaaatatctgtaaatccccatgcttatacgccaggcggacctcggaaatatgtaacagcaaccagtcatcatattgttgagtgggcggccaaaaaaggtattcctctcatctttaagtgggatgattctaatgatgttagatatgaatatgctgaaagatataaagccgttgcggataaatatgacgttgacctatcagagatagaccatcagttaatgatattagttaactataacgaagatagtaataaagctaaacaagagacgcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaatttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaattatacggagtgtataactgcggctaagttggcaattgaaaagtgtggtgcgaaaagtgtattgctgtcctttgaaccaatgaatgatttgatgagccaaaaaaatgtaatcaatattgttgatgataatattaagaagtaccacatggaatatacctaataa
BBa_K322007_sequence
1
gagattattatgttcaaaggtatagttcagggcactggaataattaaaaaaatatcaaaaaatgatgatacccaaagacatggcattacttttccaaaaaatatattggacttagttgcaaaagatactgtcatgctcgtaaatggatgctcattaactgttgttcgcatcacaggtgatgtcgtttatttcgatatagatcaggcacttaataccacaacctttgattcattagaaatcggtgacaaggtaaacttagaactacgtccagaatttggctcactccttgggaaaggtgctttaactggaaatataaagggggtagctactgttgataatattactgaagaagaagatcttcttaaagtttatattgaaattcctaaaaacctaaccaaaaatatttccgcagaagatcatattggaattaatggtgtatctaattctattgaagaagtttccaatgatattatttgcattaattatcccaaaaatttatctattaccaccaaccttagcactttagaagcaggttcgaaggttaacgtagaaacattaaatgtatcaaatgaatggtaataa
BBa_J33207_sequence
1
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga
BBa_K322149_sequence
1
ctcaaatagcaatataaggactctctatgaaatttggaaactttttgcttacataccaacctccccaattttctcaaacagaggtaatgaaacgtttggttaaattaggtcgcatctctgaggagtgtggttttgataccgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggcgcgactaaaaaattgaatgtaggaactgccgctattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgagggctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcgcgccttagcggaatgctggtacgggctgataaagaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaaccccgcggcgtatagcagaggtggcgcaccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctcaatttggcctaccgatgatattaagttggattataaatactaacgaaaagaaagcacaacttgagctttataatgaagtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtagatcatgactcaattaaagcgaaagagatttgccggaaatttctggggcattggtatgattcttatgtgaatgctacgactatttttgatgattcagaccaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgtattgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtattgacataattcaaaaagacattgatgctacaggaatatcaaatatttgttgtggatttgaagctaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgctattatattagctaaggagaaagaaatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttagtgtatgaaaatcatttttcagataatggtgttgtcggcgctcctctgactgtttctggttttctgctcggtttaacagagaaaattaaaattggttcattaaatcacatcattacaactcatcatcctgtcgccatagcggaggaagcttgcttattggatcagttaagtgaagggagatttattttagggtttagtgattgcgaaaaaaaagatgaaatgcatttttttaatcgcccggttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatccagataacgatttttatagcttccctaaaatatctgtaaatccccatgcttatacgccaggcggacctcggaaatatgtaacagcaaccagtcatcatattgttgagtgggcggccaaaaaaggtattcctctcatctttaagtgggatgattctaatgatgttagatatgaatatgctgaaagatataaagccgttgcggataaatatgacgttgacctatcagagatagaccatcagttaatgatattagttaactataacgaagatagtaataaagctaaacaagagacgcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaatttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaattatacggagtgtataactgcggctaagttggcaattgaaaagtgtggtgcgaaaagtgtattgctgtcctttgaaccaatgaatgatttgatgagccaaaaaaatgtaatcaatattgttgatgataatattaagaagtaccacatggaatatacctaataatactagaggagattattatgttcaaaggtatagttcagggcactggaataattaaaaaaatatcaaaaaatgatgatacccaaagacatggcattacttttccaaaaaatatattggacttagttgcaaaagatactgtcatgctcgtaaatggatgctcattaactgttgttcgcatcacaggtgatgtcgtttatttcgatatagatcaggcacttaataccacaacctttgattcattagaaatcggtgacaaggtaaacttagaactacgtccagaatttggctcactccttgggaaaggtgctttaactggaaatataaagggggtagctactgttgataatattactgaagaagaagatcttcttaaagtttatattgaaattcctaaaaacctaaccaaaaatatttccgcagaagatcatattggaattaatggtgtatctaattctattgaagaagtttccaatgatattatttgcattaattatcccaaaaatttatctattaccaccaaccttagcactttagaagcaggttcgaaggttaacgtagaaacattaaatgtatcaaatgaatggtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z