LOCUS       BBa_K322212             2280 bp    DNA     linear       22-Dec-2024
DEFINITION  Lac promoter + 356K firefly luciferase mutant (red colour)
ACCESSION   BBa_K322212
VERSION     BBa_K322212.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..611
                     /label=SacI
     RBS             612..618
                     /label=rbs
     RBS             609..618
                     /label=BBa_J15001
     misc_feature    1690..1692
                     /label=Introduced lysine
     CDS             625..2280
                     /label=BBa_K322211
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaggaggta ctagatggaa gacgccaaaa acataaagaa aggcccggcg
      661 ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
      721 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact
      781 tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
      841 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
      901 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
      961 gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg
     1021 ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg
     1081 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     1141 cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt
     1201 gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat
     1261 agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     1321 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     1381 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     1441 tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc
     1501 tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     1561 gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat
     1621 ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca
     1681 cccgagggga aagatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     1741 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
     1801 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     1861 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     1921 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
     1981 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
     2041 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     2101 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     2161 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     2221 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa
//