LOCUS       BBa_K322247             2277 bp    DNA     linear       22-Dec-2024
DEFINITION  Lac promoter + S284T firefly luciferase  mutant (red colour)
ACCESSION   BBa_K322247
VERSION     BBa_K322247.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    609..611
                     /label=SacI
     RBS             612..618
                     /label=rbs
     RBS             609..618
                     /label=BBa_J15001
     misc_feature    1474..1476
                     /label=Serine -> Threonine
     CDS             625..2277
                     /label=BBa_K322246
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagct caaggaggta ctagatggaa gacgccaaaa acataaagaa aggcccggcg
      661 ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
      721 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact
      781 tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
      841 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
      901 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
      961 gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg
     1021 ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg
     1081 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     1141 cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt
     1201 gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat
     1261 agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     1321 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     1381 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     1441 tttctgagga gccttcagga ttacaagatt caaactgcgc tgctggtgcc aaccctattc
     1501 tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     1561 gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat
     1621 ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca
     1681 cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag
     1741 gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg
     1801 agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt
     1861 gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc
     1921 ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct
     1981 gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt
     2041 cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg
     2101 atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg
     2161 cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca
     2221 agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaa
//