BBa_K322451 1 BBa_K322451 Photinus pyralis luciferase mutated for increased bioluminescence. 2010-08-31T11:00:00Z 2015-05-08T01:12:00Z DNA was synthesised to have the same amino acids as the wild type except for the three mutations. Mutations Ile423Leu Asp436Gly and Leu530Arg increase the bioluminescence intensity by a factor of 12.5 according to Fujii et al. (2007). It is codon optimised for expression in E. coli. Reference Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007). Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. false false _441_ 0 7053 9 Not in stock true Wild type sequence with three mutations which increase relative bioluminescence. Reference Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007). Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. false William Rostain annotation2098105 1 Brighter luciferase range2098105 1 19 1674 annotation2098104 1 B0034 range2098104 1 1 12 BBa_K322451_sequence 1 aaagaggagaaatactagatggaggacgccaaaaacatcaaaaaaggtccggcaccgttctatccactggaagatggtacagcaggtgaacagctgcataaagccatgaaacgctatgctctggttccgggtacaattgcctttaccgatgcccatatcgaggttaacatcacgtatgccgaatatttcgagatgagcgttcgtctggcagaagcaatgaaacgctatggcctgaataccaaccaccgtattgttgtgtgtagcgaaaactccctgcaattcttcatgcctgttctgggagcactgtttatcggagtggcagttgccccagcaaatgacatttataacgagcgtgagctgctgaatagtatggggattagccagccgactgtggtgtttgtgtctaaaaaaggtctgcaaaaaatcctgaacgtccaaaaaaaactgccgatcatccagaaaatcattatcatggactccaaaaccgactatcaggggttccaatcgatgtataccttcgtgacgtcacatctgcctccaggttttaatgagtatgacttcgtccctgaaagctttgatcgtgacaaaaccattgccctgatcatgaatagtagtggcagcacaggtctgcctaaaggtgttgccctgcctcatcgtacagcttgtgtccgttttagccatgcccgtgatccgatctttggcaatcagatcattcctgacaccgctatcctgagtgttgtcccgttccaccatgggtttggcatgtttaccactctgggctatctgatctgtggtttccgtgttgttctgatgtatcgctttgaggaagaactgtttctgcgtagcctgcaagactataaaatccagtccgctctgctggtgcctacactgttctcgttctttgctaaaagcacgctgatcgacaaatatgacctgagcaacctgcacgaaattgctagtggtggtgcccctctgagtaaagaagttggcgaagccgtcgctaaacgttttcatctgccgggtatccgtcaaggttatggtctgaccgaaacgacaagcgctatcctgattacaccggaaggtgacgacaaaccaggtgctgtgggaaaagttgtccctttcttcgaggcgaaagtcgtggatctggataccggtaaaaccctgggcgttaatcaacgtggagagctgtgtgttcgtggtcctatgatcatgtccggctatgtgaataatccggaggccaccaatgccctgattgataaagacggttggctgcatagtggagatctggcatattgggatgaggacgagcatttctttattgtgggtcgcctgaaaagcctgatcaaatataaaggctatcaagtggcaccggctgaactggaaagcattctgctgcaacatcctaacatctttgacgccggcgtagcaggactgcctgatgatgacgctggtgagctgccggctgccgttgttgtactggagcatgggaaaaccatgaccgagaaagagatcgtcgattatgtggcaagccaagtgactacagcgaaaaaactgcgtggtggtgtcgtgtttgttgacgaggttccgaaaggactgaccgggaaacgtgacgcccgtaaaattcgtgagattctgattaaagcaaaaaaaggcggcaaatccaaactgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z