BBa_K322624 1 BBa_K322624 Firefly luciferase S284T mutant 2010-09-23T11:00:00Z 2015-05-08T01:12:00Z Photinus pyralis This is the same as the previous BioBrick but mutation S284T was introduced, which has been reported to result in a red-shifted output. Features: S284T mutation 850 to 852 reference: true false _441_ 0 6225 9 Discontinued false Sequence date: initial introduction of this mutation into K216015 (with PyeaR promoter and RBS) failed (sequence of 123 Aug 2010) and was repeated by Will and Lu. Positive results confirming the mutation were obtained by Lu for 9 of 14 clones on 7 Sep 2010. The mutated coding sequence was then amplified and cloned in pSB1C3. Sequence confirmation 22 Sep 2010; forward sequence is fine, reverse sequence good but missing the starting T of the suffix - goes straight from TAA to ACTAGT rather than TAATACTAGT. Compared to other mistakes in submitted BioBricks this is a pretty minor one, but still annoying. false Meng Lu igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z