LOCUS       BBa_K322710             3476 bp    DNA     linear       16-Jun-2024
DEFINITION  Marker construct which can be used to target tnaA in E.coli.
ACCESSION   BBa_K322710
VERSION     BBa_K322710.1  
FEATURES             Location/Qualifiers
     misc_feature    1..478
                     /label=BBa_K322705
     RBS             766..771
                     /label=RBS
     CDS             780..1430
                     /label=cat
     misc_feature    487..1567
                     /label=BBa_K322210
     RBS             1576..1578
                     /label=RBS
     CDS             1588..3009
                     /label=Coding sequence
     misc_feature    1786..1791
                     /label=Removed EcoRI site
     misc_feature    1576..3012
                     /label=BBa_K322921
     misc_feature    487..3012
                     /label=BBa_K322922
     misc_feature    3021..3476
                     /label=BBa_K322706
ORIGIN
        1 atgcggcttc gcttcattgt taccactcct gttattcctc aacccttttt ttaaacatta
       61 aaattcttac gtaatttata atctttaaaa aaagcattta atattgctcc ccgaacgatt
      121 gtgattcgat tcacatttaa acaatttcag aatagacaaa aactctgagt gtaataatgt
      181 agcctcgtgt cttgcgagga taagtgcatt atgaatatct tacatatatg tgtgacctca
      241 aaatggttca atattgacaa caaaattgtc gatcaccgcc cttgatttgc ccttctgtag
      301 ccatcaccag agccaaaccg attagattca atgtgatcta tttgtttgct atatcttaat
      361 tttgcctttt gcaaaggtca tctctcgttt atttacttgt tttagtaaat gatggtgctt
      421 gcatatatat ctggcgaatt aatcggtata gcagatgtaa tattcacagg gatcactgta
      481 ctagagtcta tcccggcaat agttaccctt attatcaaga taagaaagaa aaggattttt
      541 cgctacgctc aaatccttta aaaaaacaca aaagaccaca ttttttaatg tggtctttat
      601 tcttcaacta aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt
      661 aaaatatata tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag
      721 aaagcagaca agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa
      781 tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
      841 tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
      901 acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
      961 taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
     1021 ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
     1081 ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
     1141 aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
     1201 tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
     1261 attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
     1321 taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
     1381 tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa
     1441 tgccgactgt actttttaca gtcggttttc taaaacgata cattaatagg tacgaaaaag
     1501 caactttttt tgcgcttaaa accagtcata ccaataactt aagggtaact agcctcgccg
     1561 gaaagagtac tagaggagac atgaacgatg aacatcaaaa agtttgcaaa acaagcaaca
     1621 gtattaacct ttactaccgc actgctggca ggaggcgcaa ctcaagcgtt tgcgaaagaa
     1681 acgaaccaaa agccatataa ggaaacatac ggcatttccc atattacacg ccatgatatg
     1741 ctgcaaatcc ctgaacagca aaaaaatgaa aaatatcaag ttcctgagtt cgattcgtcc
     1801 acaattaaaa atatctcttc tgcaaaaggc ctggacgttt gggacagctg gccattacaa
     1861 aacgctgacg gcactgtcgc aaactatcac ggctaccaca tcgtctttgc attagccgga
     1921 gatcctaaaa atgcggatga cacatcgatt tacatgttct atcaaaaagt cggcgaaact
     1981 tctattgaca gctggaaaaa cgctggccgc gtctttaaag acagcgacaa attcgatgca
     2041 aatgattcta tcctaaaaga ccaaacacaa gaatggtcag gttcagccac atttacatct
     2101 gacggaaaaa tccgtttatt ctacactgat ttctccggta aacattacgg caaacaaaca
     2161 ctgacaactg cacaagttaa cgtatcagca tcagacagct ctttgaacat caacggtgta
     2221 gaggattata aatcaatctt tgacggtgac ggaaaaacgt atcaaaatgt acagcagttc
     2281 atcgatgaag gcaactacag ctcaggcgac aaccatacgc tgagagatcc tcactacgta
     2341 gaagataaag gccacaaata cttagtattt gaagcaaaca ctggaactga agatggctac
     2401 caaggcgaag aatctttatt taacaaagca tactatggca aaagcacatc attcttccgt
     2461 caagaaagtc aaaaacttct gcaaagcgat aaaaaacgca cggctgagtt agcaaacggc
     2521 gctctcggta tgattgagct aaacgatgat tacacactga aaaaagtgat gaaaccgctg
     2581 attgcatcta acacagtaac agatgaaatt gaacgcgcga acgtctttaa aatgaacggc
     2641 aaatggtacc tgttcactga ctcccgcgga tcaaaaatga cgattgacgg cattacgtct
     2701 aacgatattt acatgcttgg ttatgtttct aattctttaa ctggcccata caagccgctg
     2761 aacaaaactg gccttgtgtt aaaaatggat cttgatccta acgatgtaac ctttacttac
     2821 tcacacttcg ctgtacctca agcgaaagga aacaatgtcg tgattacaag ctatatgaca
     2881 aacagaggat tctacgcaga caaacaatca acgtttgcgc caagcttcct gctgaacatc
     2941 aaaggcaaga aaacatctgt tgtcaaagac agcatccttg aacaaggaca attaacagtt
     3001 aacaaataat aatactagag tggctataag gatgttagcc actctcttac cctacatcct
     3061 caataacaaa aatagccttc ctctaaaggt ggcatcatga ctgatcaagc tgaaaaaaag
     3121 cactctgcat tttggggtgt tatggttata gcaggtacag taattggtgg aggtatgttt
     3181 gctttacctg ttgatcttgc cggtgcctgg tttttctggg gtgcctttat ccttatcatt
     3241 gcctggtttt caatgcttca ttccgggtta ttgttattag aagcaaattt aaattatccc
     3301 gtcggctcca gttttaacac catcaccaaa gatttaatcg gtaacacctg gaacattatc
     3361 agcggtatta ccgttgcctt cgttctctat atcctcactt atgcctatat ctctgctaat
     3421 ggtgcgatca ttagtgaaac gatatcaatg aatttgggtt atcacgctaa tccacg
//