LOCUS       BBa_K322921             1437 bp    DNA     linear       22-Nov-2024
DEFINITION  B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.
ACCESSION   BBa_K322921
VERSION     BBa_K322921.1  
FEATURES             Location/Qualifiers
     RBS             1..3
                     /label=RBS
     CDS             13..1434
                     /label=Coding sequence
     misc_feature    211..216
                     /label=Removed EcoRI site
ORIGIN
        1 gagacatgaa cgatgaacat caaaaagttt gcaaaacaag caacagtatt aacctttact
       61 accgcactgc tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca
      121 tataaggaaa catacggcat ttcccatatt acacgccatg atatgctgca aatccctgaa
      181 cagcaaaaaa atgaaaaata tcaagttcct gagttcgatt cgtccacaat taaaaatatc
      241 tcttctgcaa aaggcctgga cgtttgggac agctggccat tacaaaacgc tgacggcact
      301 gtcgcaaact atcacggcta ccacatcgtc tttgcattag ccggagatcc taaaaatgcg
      361 gatgacacat cgatttacat gttctatcaa aaagtcggcg aaacttctat tgacagctgg
      421 aaaaacgctg gccgcgtctt taaagacagc gacaaattcg atgcaaatga ttctatccta
      481 aaagaccaaa cacaagaatg gtcaggttca gccacattta catctgacgg aaaaatccgt
      541 ttattctaca ctgatttctc cggtaaacat tacggcaaac aaacactgac aactgcacaa
      601 gttaacgtat cagcatcaga cagctctttg aacatcaacg gtgtagagga ttataaatca
      661 atctttgacg gtgacggaaa aacgtatcaa aatgtacagc agttcatcga tgaaggcaac
      721 tacagctcag gcgacaacca tacgctgaga gatcctcact acgtagaaga taaaggccac
      781 aaatacttag tatttgaagc aaacactgga actgaagatg gctaccaagg cgaagaatct
      841 ttatttaaca aagcatacta tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa
      901 cttctgcaaa gcgataaaaa acgcacggct gagttagcaa acggcgctct cggtatgatt
      961 gagctaaacg atgattacac actgaaaaaa gtgatgaaac cgctgattgc atctaacaca
     1021 gtaacagatg aaattgaacg cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc
     1081 actgactccc gcggatcaaa aatgacgatt gacggcatta cgtctaacga tatttacatg
     1141 cttggttatg tttctaattc tttaactggc ccatacaagc cgctgaacaa aactggcctt
     1201 gtgttaaaaa tggatcttga tcctaacgat gtaaccttta cttactcaca cttcgctgta
     1261 cctcaagcga aaggaaacaa tgtcgtgatt acaagctata tgacaaacag aggattctac
     1321 gcagacaaac aatcaacgtt tgcgccaagc ttcctgctga acatcaaagg caagaaaaca
     1381 tctgttgtca aagacagcat ccttgaacaa ggacaattaa cagttaacaa ataataa
//