LOCUS       BBa_K322922             2526 bp    DNA     linear       29-Nov-2024
DEFINITION  cat + sacB for BRIDGE: A biobrick compatible markerless 
            recombineering method
ACCESSION   BBa_K322922
VERSION     BBa_K322922.1  
FEATURES             Location/Qualifiers
     RBS             280..285
                     /label=RBS
     CDS             294..944
                     /label=cat
     misc_feature    1..1081
                     /label=BBa_K322210
     RBS             1090..1092
                     /label=RBS
     CDS             1102..2523
                     /label=Coding sequence
     misc_feature    1300..1305
                     /label=Removed EcoRI site
     misc_feature    1090..2526
                     /label=BBa_K322921
ORIGIN
        1 tctatcccgg caatagttac ccttattatc aagataagaa agaaaaggat ttttcgctac
       61 gctcaaatcc tttaaaaaaa cacaaaagac cacatttttt aatgtggtct ttattcttca
      121 actaaagcac ccattagttc aacaaacgaa aattggataa agtgggatat ttttaaaata
      181 tatatttatg ttacagtaat attgactttt aaaaaaggat tgattctaat gaagaaagca
      241 gacaagtaag cctcctaaat tcactttaga taaaaattta ggaggcatat caaatgaact
      301 ttaataaaat tgatttagac aattggaaga gaaaagagat atttaatcat tatttgaacc
      361 aacaaacgac ttttagtata accacagaaa ttgatattag tgttttatac cgaaacataa
      421 aacaagaagg atataaattt taccctgcat ttattttctt agtgacaagg gtgataaact
      481 caaatacagc ttttagaact ggttacaata gcgacggaga gttaggttat tgggataagt
      541 tagagccact ttatacaatt tttgatggtg tatctaaaac attctctggt atttggactc
      601 ctgtaaagaa tgacttcaaa gagttttatg atttatacct ttctgatgta gagaaatata
      661 atggttcggg gaaattgttt cccaaaacac ctatacctga aaatgctttt tctctttcta
      721 ttattccatg gacttcattt actgggttta acttaaatat caataataat agtaattacc
      781 ttctacccat tattacagca ggaaaattca ttaataaagg taattcaata tatttaccgc
      841 tatctttaca ggtacatcat tctgtttgtg atggttatca tgcaggattg tttatgaact
      901 ctattcagga attgtcagat aggcctaatg actggctttt ataatatgag ataatgccga
      961 ctgtactttt tacagtcggt tttctaaaac gatacattaa taggtacgaa aaagcaactt
     1021 tttttgcgct taaaaccagt cataccaata acttaagggt aactagcctc gccggaaaga
     1081 gtactagagg agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta
     1141 acctttacta ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac
     1201 caaaagccat ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa
     1261 atccctgaac agcaaaaaaa tgaaaaatat caagttcctg agttcgattc gtccacaatt
     1321 aaaaatatct cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct
     1381 gacggcactg tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct
     1441 aaaaatgcgg atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt
     1501 gacagctgga aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat
     1561 tctatcctaa aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga
     1621 aaaatccgtt tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca
     1681 actgcacaag ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat
     1741 tataaatcaa tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat
     1801 gaaggcaact acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat
     1861 aaaggccaca aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc
     1921 gaagaatctt tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa
     1981 agtcaaaaac ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc
     2041 ggtatgattg agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca
     2101 tctaacacag taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg
     2161 tacctgttca ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat
     2221 atttacatgc ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa
     2281 actggccttg tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac
     2341 ttcgctgtac ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga
     2401 ggattctacg cagacaaaca atcaacgttt gcgccaagct tcctgctgaa catcaaaggc
     2461 aagaaaacat ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa
     2521 taataa
//