LOCUS       BBa_K322923             2412 bp    DNA     linear       26-Dec-2024
DEFINITION  Kanamycin resistance plus sacB for use the BRIDGE protocol
ACCESSION   BBa_K322923
VERSION     BBa_K322923.1  
FEATURES             Location/Qualifiers
     CDS             149..967
                     /label=kanamycin resistance
     misc_feature    1..967
                     /label=BBa_P1003
     RBS             976..978
                     /label=RBS
     CDS             988..2409
                     /label=Coding sequence
     misc_feature    1186..1191
                     /label=Removed EcoRI site
     misc_feature    976..2412
                     /label=BBa_K322921
ORIGIN
        1 ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa
       61 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
      121 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc
      181 ccgtccgcgc ttaaactcca acatggacgc tgatttatat gggtataaat gggctcgcga
      241 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
      301 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtccg
      361 tctcaactgg ctgacggagt ttatgcctct cccgaccatc aagcatttta tccgtactcc
      421 tgatgatgcg tggttactca ccaccgcgat tcctgggaaa acagccttcc aggtattaga
      481 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gccgtgttcc tgcgccggtt
      541 acattcgatt cctgtttgta attgtccttt taacagcgat cgtgtatttc gtcttgctca
      601 ggcgcaatca cgcatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
      661 tggctggcct gttgaacaag tctggaaaga aatgcacaag ctcttgccat tctcaccgga
      721 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
      781 aataggttgt attgatgttg gacgggtcgg aatcgcagac cgttaccagg accttgccat
      841 tctttggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
      901 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
      961 ctaataatac tagaggagac atgaacgatg aacatcaaaa agtttgcaaa acaagcaaca
     1021 gtattaacct ttactaccgc actgctggca ggaggcgcaa ctcaagcgtt tgcgaaagaa
     1081 acgaaccaaa agccatataa ggaaacatac ggcatttccc atattacacg ccatgatatg
     1141 ctgcaaatcc ctgaacagca aaaaaatgaa aaatatcaag ttcctgagtt cgattcgtcc
     1201 acaattaaaa atatctcttc tgcaaaaggc ctggacgttt gggacagctg gccattacaa
     1261 aacgctgacg gcactgtcgc aaactatcac ggctaccaca tcgtctttgc attagccgga
     1321 gatcctaaaa atgcggatga cacatcgatt tacatgttct atcaaaaagt cggcgaaact
     1381 tctattgaca gctggaaaaa cgctggccgc gtctttaaag acagcgacaa attcgatgca
     1441 aatgattcta tcctaaaaga ccaaacacaa gaatggtcag gttcagccac atttacatct
     1501 gacggaaaaa tccgtttatt ctacactgat ttctccggta aacattacgg caaacaaaca
     1561 ctgacaactg cacaagttaa cgtatcagca tcagacagct ctttgaacat caacggtgta
     1621 gaggattata aatcaatctt tgacggtgac ggaaaaacgt atcaaaatgt acagcagttc
     1681 atcgatgaag gcaactacag ctcaggcgac aaccatacgc tgagagatcc tcactacgta
     1741 gaagataaag gccacaaata cttagtattt gaagcaaaca ctggaactga agatggctac
     1801 caaggcgaag aatctttatt taacaaagca tactatggca aaagcacatc attcttccgt
     1861 caagaaagtc aaaaacttct gcaaagcgat aaaaaacgca cggctgagtt agcaaacggc
     1921 gctctcggta tgattgagct aaacgatgat tacacactga aaaaagtgat gaaaccgctg
     1981 attgcatcta acacagtaac agatgaaatt gaacgcgcga acgtctttaa aatgaacggc
     2041 aaatggtacc tgttcactga ctcccgcgga tcaaaaatga cgattgacgg cattacgtct
     2101 aacgatattt acatgcttgg ttatgtttct aattctttaa ctggcccata caagccgctg
     2161 aacaaaactg gccttgtgtt aaaaatggat cttgatccta acgatgtaac ctttacttac
     2221 tcacacttcg ctgtacctca agcgaaagga aacaatgtcg tgattacaag ctatatgaca
     2281 aacagaggat tctacgcaga caaacaatca acgtttgcgc caagcttcct gctgaacatc
     2341 aaaggcaaga aaacatctgt tgtcaaagac agcatccttg aacaaggaca attaacagtt
     2401 aacaaataat aa
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