LOCUS       BBa_K322924             3362 bp    DNA     linear       22-Nov-2024
DEFINITION  Alternate targeting contruct for TnaA replacement using BRIDGE
ACCESSION   BBa_K322924
VERSION     BBa_K322924.1  
FEATURES             Location/Qualifiers
     misc_feature    1..478
                     /label=BBa_K322705
     CDS             635..1453
                     /label=kanamycin resistance
     misc_feature    487..1453
                     /label=BBa_P1003
     RBS             1462..1464
                     /label=RBS
     CDS             1474..2895
                     /label=Coding sequence
     misc_feature    1672..1677
                     /label=Removed EcoRI site
     misc_feature    1462..2898
                     /label=BBa_K322921
     misc_feature    487..2898
                     /label=BBa_K322923
     misc_feature    2907..3362
                     /label=BBa_K322706
ORIGIN
        1 atgcggcttc gcttcattgt taccactcct gttattcctc aacccttttt ttaaacatta
       61 aaattcttac gtaatttata atctttaaaa aaagcattta atattgctcc ccgaacgatt
      121 gtgattcgat tcacatttaa acaatttcag aatagacaaa aactctgagt gtaataatgt
      181 agcctcgtgt cttgcgagga taagtgcatt atgaatatct tacatatatg tgtgacctca
      241 aaatggttca atattgacaa caaaattgtc gatcaccgcc cttgatttgc ccttctgtag
      301 ccatcaccag agccaaaccg attagattca atgtgatcta tttgtttgct atatcttaat
      361 tttgcctttt gcaaaggtca tctctcgttt atttacttgt tttagtaaat gatggtgctt
      421 gcatatatat ctggcgaatt aatcggtata gcagatgtaa tattcacagg gatcactgta
      481 ctagagctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc
      541 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
      601 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
      661 ttgctcccgt ccgcgcttaa actccaacat ggacgctgat ttatatgggt ataaatgggc
      721 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
      781 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
      841 ggtccgtctc aactggctga cggagtttat gcctctcccg accatcaagc attttatccg
      901 tactcctgat gatgcgtggt tactcaccac cgcgattcct gggaaaacag ccttccaggt
      961 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggccg tgttcctgcg
     1021 ccggttacat tcgattcctg tttgtaattg tccttttaac agcgatcgtg tatttcgtct
     1081 tgctcaggcg caatcacgca tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     1141 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cacaagctct tgccattctc
     1201 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     1261 gaaattaata ggttgtattg atgttggacg ggtcggaatc gcagaccgtt accaggacct
     1321 tgccattctt tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     1381 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     1441 gtttttctaa taatactaga ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa
     1501 gcaacagtat taacctttac taccgcactg ctggcaggag gcgcaactca agcgtttgcg
     1561 aaagaaacga accaaaagcc atataaggaa acatacggca tttcccatat tacacgccat
     1621 gatatgctgc aaatccctga acagcaaaaa aatgaaaaat atcaagttcc tgagttcgat
     1681 tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg acgtttggga cagctggcca
     1741 ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct accacatcgt ctttgcatta
     1801 gccggagatc ctaaaaatgc ggatgacaca tcgatttaca tgttctatca aaaagtcggc
     1861 gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc
     1921 gatgcaaatg attctatcct aaaagaccaa acacaagaat ggtcaggttc agccacattt
     1981 acatctgacg gaaaaatccg tttattctac actgatttct ccggtaaaca ttacggcaaa
     2041 caaacactga caactgcaca agttaacgta tcagcatcag acagctcttt gaacatcaac
     2101 ggtgtagagg attataaatc aatctttgac ggtgacggaa aaacgtatca aaatgtacag
     2161 cagttcatcg atgaaggcaa ctacagctca ggcgacaacc atacgctgag agatcctcac
     2221 tacgtagaag ataaaggcca caaatactta gtatttgaag caaacactgg aactgaagat
     2281 ggctaccaag gcgaagaatc tttatttaac aaagcatact atggcaaaag cacatcattc
     2341 ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc tgagttagca
     2401 aacggcgctc tcggtatgat tgagctaaac gatgattaca cactgaaaaa agtgatgaaa
     2461 ccgctgattg catctaacac agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg
     2521 aacggcaaat ggtacctgtt cactgactcc cgcggatcaa aaatgacgat tgacggcatt
     2581 acgtctaacg atatttacat gcttggttat gtttctaatt ctttaactgg cccatacaag
     2641 ccgctgaaca aaactggcct tgtgttaaaa atggatcttg atcctaacga tgtaaccttt
     2701 acttactcac acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat tacaagctat
     2761 atgacaaaca gaggattcta cgcagacaaa caatcaacgt ttgcgccaag cttcctgctg
     2821 aacatcaaag gcaagaaaac atctgttgtc aaagacagca tccttgaaca aggacaatta
     2881 acagttaaca aataataata ctagagtggc tataaggatg ttagccactc tcttacccta
     2941 catcctcaat aacaaaaata gccttcctct aaaggtggca tcatgactga tcaagctgaa
     3001 aaaaagcact ctgcattttg gggtgttatg gttatagcag gtacagtaat tggtggaggt
     3061 atgtttgctt tacctgttga tcttgccggt gcctggtttt tctggggtgc ctttatcctt
     3121 atcattgcct ggttttcaat gcttcattcc gggttattgt tattagaagc aaatttaaat
     3181 tatcccgtcg gctccagttt taacaccatc accaaagatt taatcggtaa cacctggaac
     3241 attatcagcg gtattaccgt tgccttcgtt ctctatatcc tcacttatgc ctatatctct
     3301 gctaatggtg cgatcattag tgaaacgata tcaatgaatt tgggttatca cgctaatcca
     3361 cg
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