LOCUS       BBa_K322999             1806 bp    DNA     linear       22-Nov-2024
DEFINITION  Blue light sensor (lovTAP) + Reporter system
ACCESSION   BBa_K322999
VERSION     BBa_K322999.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS B0032
     misc_feature    702..706
                     /label=stop codon
     CDS             18..701
                     /label=LovTAP K360022
     stem_loop       725..771
                     /label=Terminator B0010
     stem_loop       804..830
                     /label=Terminator B0012
     polyA_site      831..844
                     /label=AAA
     misc_feature    1..844
                     /label=BBa_K360121
     promoter        853..937
                     /label=BBa_K191007
     misc_feature    950..953
                     /label=conserved
     RBS             946..957
                     /label=BBa_B0034
     CDS             964..1638
                     /label=mrfp1
     misc_feature    1645..1669
                     /label=Help:Barcodes
     CDS             964..1644
                     /label=BBa_E1010
     stem_loop       1689..1732
                     /label=stem_loop
     misc_feature    1678..1757
                     /label=BBa_B0010
     terminator      1678..1757
                     /label=BBa_B0010
     stem_loop       1773..1792
                     /label=T7 TE
     misc_feature    1799..1799
                     /label=stop
     polyA_site      1793..1806
                     /label=polya
     misc_feature    1766..1806
                     /label=BBa_B0012
     terminator      1766..1806
                     /label=BBa_B0012
     misc_feature    946..1806
                     /label=BBa_I13507
     misc_feature    853..1806
                     /label=BBa_K191004
ORIGIN
        1 tcacacagga aagtactatg ttggctacta cacttgaacg tattgagaag aactttgtca
       61 ttactgaccc aaggttgcca gataatccct ttatattcgc gtccgatagt ttcttgcagt
      121 tgacagaata tagccgtgaa gaaattttgg gaagaaattg tcgttttcta caaggtcctg
      181 aaactgatcg cgcgacagtg agaaaaatta gagatgccat agataaccaa acagaggtca
      241 ctgttcagct gattaattat acaaagagtg gtaaaaagtt ctggaacctc tttcacttgc
      301 agcctatgcg agatcagaag ggagatgtcc agtactttat tggggttcag ttggatggaa
      361 ctgagcatgt ccgagatgct gccgagagag agggagtcat gctgattaag aaaaccgccg
      421 aaaatattga tgaggcggca tttgtcgacc tgcttaagaa tgcctaccaa aacgatctcc
      481 atttaccgtt gttaaacctg atgctgacgc cagatgagcg cgaagcgttg gggactcgcg
      541 tgcgtattgt cgaagagctg ttgcgcggcg aaatgagcca gcgtgagtta aaaaatgaac
      601 tcggcgcggg catcgcgacg attacgcgtg gatctaacag cctgaaagcc gcgcccgtgg
      661 agctgcgcca gtggctggaa gaggtgttgc tgaaaagcga ttaataatac tagagccagg
      721 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      781 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      841 tatatactag agtggcaaat attctgaaat gagctgttga caattaatca tcgaactagt
      901 taactagtac gcaagttcac gtaaaaaggg tatcgactac tagagaaaga ggagaaatac
      961 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
     1021 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
     1081 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
     1141 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
     1201 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
     1261 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
     1321 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
     1381 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
     1441 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
     1501 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
     1561 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
     1621 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
     1681 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1741 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1801 tttata
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