LOCUS       BBa_K323015              984 bp    DNA     linear       27-Dec-2024
DEFINITION  PBSII link nYFP under T7 promoter
ACCESSION   BBa_K323015
VERSION     BBa_K323015.1  
FEATURES             Location/Qualifiers
     promoter        1..17
                     /label=pT7
     misc_feature    20..44
                     /label=lacO
     RBS             76..82
                     /label=rbs
     misc_feature    88..90
                     /label=ATG
     misc_feature    91..111
                     /label=Tyr + His tag
     misc_feature    118..405
                     /label=BBa_K323107
     misc_feature    376..405
                     /label=linker
     misc_feature    412..927
                     /label=BBa_K323072
     misc_feature    925..927
                     /label=STOP
     misc_feature    934..936
                     /label=STOP
     misc_feature    937..984
                     /label=T7 term
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
       61 tgtttaactt taagaaggag gtaaataatg tatcatcacc atcaccatca cactagaccg
      121 ggtgaaaaac cgtatgcatg tcctgaatgt ggtaaaagct ttagccagcg tgcaaatctg
      181 cgtgcacatc agcgtaccca tacaggtgaa aagccttata aatgcccaga atgcggcaaa
      241 agcttttcac gtagcgatca tctgaccacc catcagcgca cacatactgg cgagaaacct
      301 tacaaatgtc cagagtgtgg taaatcattt agccgtagtg atgttctggt tcgtcatcag
      361 agaacccaca cgggtggtgg tagcggtggt ggttcaggtg gtagtactag agtgagcaag
      421 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      481 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      541 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      601 ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      661 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      721 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      781 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      841 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      901 aacttcaaga tccgccacaa catctaaact agataactag cataacccct tggggcctct
      961 aaacgggtct tgaggggttt tttg
//