LOCUS       BBa_K323058             1257 bp    DNA     linear       30-Jun-2024
DEFINITION  Gli1 link nCFP under T7 promoter
ACCESSION   BBa_K323058
VERSION     BBa_K323058.1  
FEATURES             Location/Qualifiers
     promoter        1..17
                     /label=pT7
     misc_feature    20..44
                     /label=lacO
     RBS             76..82
                     /label=RBS
     misc_feature    88..90
                     /label=ATG
     misc_feature    91..111
                     /label=Tyr + His tag
     misc_feature    118..678
                     /label=BBa_K323063
     misc_feature    649..678
                     /label=linker
     misc_feature    685..1200
                     /label=BBa_K323011
     misc_feature    1198..1200
                     /label=STOP
     misc_feature    1207..1209
                     /label=STOP
     misc_feature    1210..1257
                     /label=T7 term
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
       61 tgtttaactt taagaaggag gtaaataatg tatcatcacc atcaccatca cactagaaag
      121 cgtgagcctg aatctgtgta tgaaactgac tgccgttggg atggctgtag ccaggaattt
      181 gactcccaag agcagctggt gcaccacatc aacagcgagc acatccacgg ggagcggaag
      241 gagttcgtgt gccactgggg gggctgctcc agggagctga ggcccttcaa agcccagtac
      301 atgctggtgg ttcacatgcg cagacacact ggcgagaagc cacacaagtg cacgtttgaa
      361 gggtgccgga agtcatactc acgcctcgaa aacctgaaga cgcacctgcg gtcacacacg
      421 ggtgagaagc catacatgtg tgagcacgag ggctgtagta aagccttcag caatgccagt
      481 gaccgagcca agcaccagaa tcggacccat tccaatgaga agccgtatgt atgtaagctc
      541 cctggctgca ccaaacgcta tacagatcct agctcgctgc gaaaacatgt caagacagtg
      601 catggtcctg acgcccatgt gaccaaacgg caccgtgggg atactagagg cgggtcaggc
      661 gggggctcag gcgggtcaac tagagtgagc aagggcgagg agctgttcac cggggtggtg
      721 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      781 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      841 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc
      901 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      961 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1021 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1081 gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa cgtctatatc
     1141 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatctaa
     1201 actagataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttg
//