LOCUS BBa_K323089 4643 bp DNA linear 22-Dec-2024 DEFINITION In vivo testing device for protein-DNA binding: part 2 (lacZ_DTER_pBAD_BsaI_DTER) ACCESSION BBa_K323089 VERSION BBa_K323089.1 FEATURES Location/Qualifiers RBS 5..10 /label=RBS CDS 19..3093 /label=lacZ misc_feature 3100..3229 /label=double terminator promoter 3238..4446 /label=pBAD/araC misc_feature 4513..4642 /label=double terminator misc_feature 4467..4506 /label=BsaI clone in RBS 4458..4463 /label=RBS ORIGIN 1 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt 61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 241 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac 301 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt 361 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg 421 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg 481 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg 541 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac 601 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg 661 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat 721 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac 781 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct 841 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg 901 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt 961 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc 1021 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc 1081 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg 1141 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg 1201 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc 1261 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta 1321 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg 1381 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc 1441 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc 1501 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg 1561 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg 1621 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg 1681 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag 1741 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc 1801 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg 1861 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg 1921 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac 1981 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct 2041 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa 2101 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac 2161 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg 2221 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat 2281 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa 2341 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt 2401 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg 2461 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg 2521 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg 2581 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg 2641 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag 2701 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc 2761 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc 2821 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc 2881 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc 2941 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt 3001 ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag cgccggtcgc 3061 taccattacc agttggtctg gtgtcaaaaa taaactagac caggcatcaa ataaaacgaa 3121 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3181 actagagtca cactggctcg accttcgggt gggcctttct gcgtttatat actagagtta 3241 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg 3301 gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac 3361 attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct 3421 gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg 3481 tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct 3541 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg 3601 atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat 3661 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag 3721 gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat 3781 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg 3841 ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag 3901 caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc 3961 gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc 4021 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta 4081 tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag 4141 agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct 4201 cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac 4261 caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat 4321 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc 4381 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc gtttttttgg 4441 gctagctact agaaaagagg agaaaactag agagaccaag cttgcgcgca tgcatggtct 4501 cgggccacta gaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 4561 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcgaccttcg 4621 ggtgggcctt tctgcgttta tat //