LOCUS       BBa_K323116             2850 bp    DNA     linear       19-Nov-2024
DEFINITION  pT7_TetO+NLS_T7RNAP_PEST
ACCESSION   BBa_K323116
VERSION     BBa_K323116.1  
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label=T7 promoter
     misc_feature    19..33
                     /label=TetO
     RBS             39..51
                     /label=rbs+Kozak
     misc_feature    52..54
                     /label=start
     misc_feature    2848..2850
                     /label=stop
     misc_feature    2717..2847
                     /label=PEST
     CDS             79..2716
                     /label=T7 RNA polymerase
     misc_feature    52..78
                     /label=NLS
ORIGIN
        1 taatacgact cactatagtc tatcaatgat agaactagaa ggagggccac catggatcca
       61 aaaaagaaga gaaaggtaaa cacgattaac atcgctaaga acgacttctc tgacatcgaa
      121 ctggctgcta tcccgttcaa cactctggct gaccattacg gtgagcgttt agctcgcgaa
      181 cagttggccc ttgagcatga gtcttacgag atgggtgaag cacgcttccg caagatgttt
      241 gagcgtcaac ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc tctcatcact
      301 accctactcc ctaagatgat tgcacgcatc aacgactggt ttgaggaagt gaaagctaag
      361 cgcggcaagc gcccgacagc cttccagttc ctgcaagaaa tcaagccgga agccgtagcg
      421 tacatcacca ttaagaccac tctggcttgc ctaaccagtg ctgacaatac aaccgttcag
      481 gctgtagcaa gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt
      541 gaccttgaag ctaagcactt caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg
      601 cacgtctaca agaaagcatt tatgcaagtt gtcgaggctg acatgctctc taagggtcta
      661 ctcggtggcg aggcgtggtc ttcgtggcat aaggaagact ctattcatgt aggagtacgc
      721 tgcatcgaga tgctcattga gtcaaccgga atggttagct tacaccgcca aaatgctggc
      781 gtagtaggtc aagactctga gactatcgaa ctcgcacctg aatacgctga ggctatcgca
      841 acccgtgcag gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt agttcctcct
      901 aagccgtgga ctggcattac tggtggtggc tattgggcta acggtcgtcg tcctctggcg
      961 ctggtgcgta ctcacagtaa gaaagcactg atgcgctacg aagacgttta catgcctgag
     1021 gtgtacaaag cgattaacat tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta
     1081 gcggtcgcca acgtaatcac caagtggaag cattgtccgg tcgaggacat ccctgcgatt
     1141 gagcgtgaag aactcccgat gaaaccggaa gacatcgaca tgaatcctga ggctctcacc
     1201 gcgtggaaac gtgctgccgc tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt
     1261 atcagccttg agttcatgct tgagcaagcc aataagtttg ctaaccataa ggccatctgg
     1321 ttcccttaca acatggactg gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa
     1381 ggtaacgata tgaccaaagg actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa
     1441 ggttactact ggctgaaaat ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc
     1501 cctgagcgca tcaagttcat tgaggaaaac cacgagaaca tcatggcttg cgctaagtct
     1561 ccactggaga acacttggtg ggctgagcaa gattctccgt tctgcttcct tgcgttctgc
     1621 tttgagtacg ctggggtaca gcaccacggc ctgagctata actgctccct tccgctggcg
     1681 tttgacgggt cttgctctgg catccagcac ttctccgcga tgctccgaga tgaggtaggt
     1741 ggtcgcgcgg ttaacttgct tcctagtgaa accgttcagg acatctacgg gattgttgct
     1801 aagaaagtca acgagattct acaagcagac gcaatcaatg ggaccgataa cgaagtagtt
     1861 accgtgaccg atgagaacac tggtgaaatc tctgagaaag tcaagctggg cactaaggca
     1921 ctggctggtc aatggctggc ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg
     1981 acgctggctt acgggtccaa agagttcggc ttccgtcaac aagtgctgga agataccatt
     2041 cagccagcta ttgattccgg caagggtctg atgttcactc agccgaatca ggctgctgga
     2101 tacatggcta agctgatttg ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca
     2161 atgaactggc ttaagtctgc tgctaagctg ctggctgctg aggtcaaaga taagaagact
     2221 ggagagattc ttcgcaagcg ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg
     2281 tggcaggaat acaagaagcc tattcagacg cgcttgaacc tgatgttcct cggtcagttc
     2341 cgcttacagc ctaccattaa caccaacaaa gatagcgaga ttgatgcaca caaacaggag
     2401 tctggtatcg ctcctaactt tgtacacagc caagacggta gccaccttcg taagactgta
     2461 gtgtgggcac acgagaagta cggaatcgaa tcttttgcac tgattcacga ctccttcggt
     2521 accattccgg ctgacgctgc gaacctgttc aaagcagtgc gcgaaactat ggttgacaca
     2581 tatgagtctt gtgatgtact ggctgatttc tacgaccagt tcgctgacca gttgcacgag
     2641 tctcaattgg acaaaatgcc agcacttccg gctaaaggta acttgaacct ccgtgacatc
     2701 ttagagtcgg acttcgcgtt cgcgactaga cacggcttcc ctcccgaggt ggaggagcag
     2761 gccgccggca ccctgcccat gagctgcgcc caggagagcg gcatggatag acaccctgct
     2821 gcttgcgcca gcgccaggat caacgtctaa
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