LOCUS       BBa_K323118              822 bp    DNA     linear       19-Nov-2024
DEFINITION  pT7_TetO+NLS_TetR_PEST
ACCESSION   BBa_K323118
VERSION     BBa_K323118.1  
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label=T7 promoter
     misc_feature    19..33
                     /label=TetO
     RBS             39..51
                     /label=rbs+Kozak sequence
     misc_feature    52..54
                     /label=start
     misc_feature    55..78
                     /label=NLS
     misc_feature    820..822
                     /label=stop
     misc_feature    696..819
                     /label=PEST
     CDS             79..690
                     /label=Tet repressor
ORIGIN
        1 taatacgact cactatagtc tatcaatgat agaactagaa ggagggccac catggatcca
       61 aaaaagaaga gaaaggtatc cagattagat aaaagtaaag tgattaacag cgcattagag
      121 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
      181 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
      241 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
      301 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
      361 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
      421 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
      481 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
      541 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
      601 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
      661 ggattagaaa aacaacttaa atgtgaaagt gggtccacta gacacggctt ccctcccgag
      721 gtggaggagc aggccgccgg caccctgccc atgagctgcg cccaggagag cggcatggat
      781 agacaccctg ctgcttgcgc cagcgccagg atcaacgtct aa
//