LOCUS       BBa_K323120             1328 bp    DNA     linear       22-Dec-2024
DEFINITION  CMV+NLS_TetR
ACCESSION   BBa_K323120
VERSION     BBa_K323120.1  
FEATURES             Location/Qualifiers
     promoter        1..662
                     /label=CMV
     RBS             668..680
                     /label=rbs+Kozak sequence
     misc_feature    681..683
                     /label=start
     misc_feature    681..707
                     /label=NLS
     CDS             708..1328
                     /label=Tet repressor
     misc_feature    1326..1328
                     /label=stop
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg cctagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaatactaga
      661 gtactagaag gagggccacc atggatccaa aaaagaagag aaaggtatcc agattagata
      721 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
      781 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
      841 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
      901 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
      961 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     1021 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     1081 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     1141 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     1201 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     1261 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     1321 ggtcctaa
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