LOCUS BBa_K323135 6271 bp DNA linear 22-Dec-2024 DEFINITION VioA and VioB enzymes fused with zinc fingers under pBAD promoter ACCESSION BBa_K323135 VERSION BBa_K323135.1 FEATURES Location/Qualifiers promoter 1..1209 /label=pBAD/araC RBS 1215..1220 /label=RBS CDS 1230..1550 /label=Blues-linker CDS 1557..2817 /label=vioA RBS 2829..2834 /label=RBS CDS 2838..3134 /label=Zif268-linker CDS 3135..6141 /label=vioB misc_feature 6142..6271 /label=double terminator ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat accgtttttt 1201 tgggctagcg aaagaggaga aatactagaa tgactagagc aagtgatgat cgtccgtatg 1261 catgtccggt tgaaagctgt gatcgtcgtt ttagccgtcg tgatgttctg atgaatcaca 1321 ttcgtattca cacaggtcag aaaccgtttc agtgtcgtat ttgcatgcgt aattttagcc 1381 gtagcgatca tctgaccacc catattcgta cccatacagg tgaaaaaccg tttgcatgtg 1441 atatttgcgg tcgcaaattt gcaaatcgtg ataccctgac ccgtcatagc aaaattcatc 1501 tgcgtcagaa tgatctggaa ggtggtagcg gtggtggttc aggtggtagt actagaatga 1561 aacattcttc cgatatctgc attgttggtg ctggtatttc tggtttgacg tgcgcaagcc 1621 atctgctgga cagcccggca tgccgtggtc tgagcctgcg tatctttgac atgcagcaag 1681 aagccggtgg ccgtatccgc agcaaaatgc tggatggtaa ggcaagcatt gaactgggcg 1741 caggtcgcta ctcccctcag ttgcacccgc atttccaaag cgcaatgcag cactatagcc 1801 aaaagagcga agtctatccg ttcacccagt tgaagttcaa atctcacgtg cagcaaaagc 1861 tgaagcgcgc catgaatgaa ctgtccccgc gtctgaaaga gcatggtaaa gagagctttt 1921 tgcagtttgt cagccgttat caaggtcacg atagcgcggt tggtatgatc cgctctatgg 1981 gttacgacgc actgttcctg ccggatatca gcgcagaaat ggcctacgac attgtgggta 2041 agcacccgga gatccagagc gtgacggaca acgacgcgaa ccaatggttt gcagcggaaa 2101 cgggctttgc tggtctgatt cagggcatca aggctaaggt taaggcggca ggtgcgcgtt 2161 ttagcctggg ttatcgtctg ctgagcgtcc gtaccgacgg tgacggctac ctgctgcaac 2221 tggcaggtga cgacggctgg aaactggagc accgtacccg ccatctgatt ctggcgattc 2281 cgccgagcgc gatggcgggt ttgaatgttg attttccaga agcctggtcc ggtgcgcgct 2341 atggcagcct gccgctgttt aagggctttc tgacgtacgg tgagccgtgg tggttggact 2401 acaaactgga cgatcaggtg ctgattgttg acaacccgct gcgcaaaatc tatttcaaag 2461 gcgataagta cctgttcttc tataccgata gcgagatggc gaattactgg cgcggttgtg 2521 tcgcggaggg cgaggacggt tacctggagc aaattcgcac ccatttggct agcgcactgg 2581 gtatcgtccg tgaacgtatc ccgcaaccgc tggcacacgt tcacaagtat tgggcgcacg 2641 gcgttgagtt ttgccgtgat tctgatattg accacccgag cgcactgtct catcgcgaca 2701 gcggtatcat cgcgtgctcc gatgcgtaca cggagcattg tggttggatg gagggcggtc 2761 tgctgagcgc ccgtgaggca agccgtctgc tgttgcagcg tatcgccgcg tgattaaact 2821 agagaaagag gagaaatatg ccgggtgaaa aaccgtatgc atgtccggtt gaaagctgtg 2881 atcgtcgttt tagccgtagt gatgaactga cccgtcatat tcgtattcat acaggtcaga 2941 aaccgtttca gtgtcgtatt tgcatgcgta attttagccg ttcagatcat ctgaccaccc 3001 atattcgtac ccatactggc gaaaaaccgt ttgcctgtga tatttgtggt cgtaaatttg 3061 cacgttccga tgaacgtaaa cgccatacca aaattcatac gggtggtggt agcggtggtg 3121 gttcaggtgg tagtatgagc attctggatt tcccgcgtat ccacttccgt ggctgggccc 3181 gtgtcaatgc gccgaccgcg aaccgcgatc cgcacggcca catcgatatg gccagcaata 3241 ccgtggcgat ggcgggtgag ccgttcgacc tggcacgcca tcctacggag ttccaccgtc 3301 acctgcgctc cctgggtccg cgcttcggct tggatggtcg tgctgacccg gaaggcccgt 3361 tcagcctggc cgagggctac aacgctgccg gtaacaacca cttttcgtgg gagagcgcaa 3421 ccgttagcca cgtgcaatgg gatggcggtg aggcggatcg tggtgacggt ctggtcggtg 3481 ctcgtttggc actgtggggt cactacaatg attatctgcg taccaccttc aatcgtgctc 3541 gttgggtcga cagcgacccg acgcgccgtg acgctgcaca aatctatgcg ggccaattca 3601 ccattagccc ggctggtgcc ggtccgggta cgccgtggct gtttacggca gacattgatg 3661 atagccatgg tgcacgttgg acgcgtggcg gccacattgc agagcgtggc ggccacttct 3721 tggatgaaga gtttggtctg gcacgcctgt ttcagttctc tgtgccgaaa gatcacccac 3781 attttctgtt tcacccgggt ccgtttgatt ccgaggcctg gcgtcgtctg caattggctc 3841 tggaggatga cgacgttctg ggtctgaccg tgcaatatgc gttgttcaat atgagcaccc 3901 cgcctcagcc gaacagcccg gtttttcacg atatggtcgg tgttgtcggt ctgtggcgtc 3961 gtggtgaact ggcgagctac ccggctggtc gtctgctgcg tccgcgtcaa ccgggtctgg 4021 gtgacctgac cctgcgcgtc aacggtggtc gcgttgcgct gaatttggcg tgtgccattc 4081 cgttcagcac tcgtgccgcg cagccaagcg caccggaccg cctgaccccg gacctgggtg 4141 ccaaactgcc gctgggcgat ctgctgctgc gtgatgagga cggcgcactg ttggcacgtg 4201 tgccgcaggc tctgtaccaa gactattgga cgaatcacgg tattgtggac ctgccgctgc 4261 tgcgcgaacc gcgtggtagc ttgaccctga gcagcgaact ggcggagtgg cgtgagcaag 4321 actgggtcac ccaaagcgac gcgtctaacc tgtacctgga ggcaccggat cgccgtcacg 4381 gtcgcttttt ccctgagagc atcgcgctgc gcagctactt tcgcggtgaa gcgcgtgcgc 4441 gtccggatat cccgcatcgt atcgagggca tgggcctggt cggcgtcgaa tctcgtcagg 4501 atggcgacgc tgcggaatgg cgtctgacgg gtctgcgtcc gggtccggca cgcattgttc 4561 tggacgatgg tgccgaggcg atccctctgc gtgttctgcc tgacgattgg gcgctggatg 4621 acgcgaccgt cgaagaagtg gattacgcct ttttgtaccg ccacgttatg gcgtattacg 4681 agctggtgta tccattcatg agcgacaagg tgttttccct ggctgatcgt tgcaaatgtg 4741 aaacgtacgc acgtctgatg tggcagatgt gtgatccgca gaaccgcaac aagtcctatt 4801 acatgccgag cacccgcgaa ctgtcggcac cgaaagctcg tttgttcttg aagtatctgg 4861 cccacgtgga aggccaggca cgcctgcaag cacctccgcc agcgggtccg gcacgcattg 4921 aatctaaagc ccagttggcg gcagagctgc gtaaagccgt cgacctggag ctgtctgtga 4981 tgctgcaata cctgtacgcg gcgtatagca ttccgaacta tgcacagggc caacaacgtg 5041 ttcgtgacgg tgcgtggacc gccgagcagc tgcaactggc gtgcggtagc ggtgaccgtc 5101 gccgtgatgg cggtattcgt gcagcactgc tggaaattgc tcatgaagaa atgattcatt 5161 acctggtcgt taacaacctg ctgatggccc tgggcgagcc gttctacgcg ggtgtcccgc 5221 tgatgggcga agcggcacgt caggcgtttg gcctggacac cgagttcgct ctggaaccgt 5281 ttagcgaaag cacgctggca cgttttgttc gtctggaatg gccgcacttt atcccagcac 5341 cgggcaaatc catcgcggac tgctatgccg ccattcgtca ggcgtttttg gatctgccgg 5401 acttgtttgg tggcgaggca ggtaagcgtg gcggtgaaca ccacctgttc ctgaatgagc 5461 tgaccaaccg tgcgcatccg ggttatcaac tggaagtttt cgatcgcgac tcggcgctgt 5521 ttggtattgc atttgtgacc gatcagggcg aaggtggcgc tctggacagc ccgcactacg 5581 aacatagcca ttttcaacgt ctgcgtgaaa tgagcgcgcg tatcatggct caaagcgcac 5641 cgttcgaacc ggcgctgccg gcgttgcgta atccggttct ggatgagagc ccgggttgcc 5701 aacgtgtcgc agacggtcgt gcgcgtgcgc tgatggcatt gtaccaaggc gtttatgagc 5761 tgatgtttgc gatgatggcg cagcacttcg ccgtgaaacc gctgggtagc ttgcgtcgca 5821 gccgcctgat gaacgcagca atcgatctga tgaccggtct gttgcgtccg ctgagctgcg 5881 cgctgatgaa cctgccaagc ggcatcgccg gtcgcacggc cggtccgccg ctgccgggtc 5941 cggttgacac ccgtagctat gacgactacg cgctgggctg tcgcatgctg gcacgccgtt 6001 gcgagcgtct gctggagcag gcgagcatgc tggaaccggg ttggctgccg gatgcgcaga 6061 tggagctgct ggatttctat cgtcgccaaa tgctggactt ggcgtgcggc aaactgagcc 6121 gcgaggccta aggatcctta accaggcatc aaataaaacg aaaggctcag tcgaaagact 6181 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 6241 cgaccttcgg gtgggccttt ctgcgtttat a //