LOCUS BBa_K323138 6275 bp DNA linear 26-Nov-2024 DEFINITION R0011 crtEBIYO ACCESSION BBa_K323138 VERSION BBa_K323138.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..67 /label=BBa_K118012 CDS 74..985 /label=BBa_I742151 misc_feature 994..996 /label=SacI RBS 997..1003 /label=rbs RBS 994..1003 /label=BBa_J15001 CDS 1010..1939 /label=crtB coding sequence CDS 1010..1942 /label=BBa_K118002 misc_feature 1951..1953 /label=SacI RBS 1954..1960 /label=rbs RBS 1951..1960 /label=BBa_J15001 misc_feature 2444..2449 /label=PstI site removed misc_feature 2855..2860 /label=PstI site removed CDS 1967..3445 /label=crtI coding sequence CDS 1967..3448 /label=BBa_K118003 RBS 3457..3460 /label=BBa_K118012 CDS 3467..4615 /label=crtY coding sequence CDS 3467..4618 /label=BBa_K118008 misc_feature 1..4618 /label=BBa_K274210 RBS 4632..4638 /label=Elowitz RBS misc_feature 4646..6275 /label=crtO misc_feature 4627..6275 /label=BBa_K323213 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gaggaggtac tagatgacgg tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc 121 ggagcagtta ctggctgata ttgatcgacg ccttgatcag ttattgcccg tggagggaga 181 acgggatgtt gtgggtgccg cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg 241 ccccatgttg ctgttgctga ccgcccgcga tctgggttgc gctgtcagcc atgacggatt 301 actggatttg gcctgtgcgg tggaaatggt ccacgcggct tcgctgatcc ttgacgatat 361 gccctgcatg gacgatgcga agctgcggcg cggacgccct accattcatt ctcattacgg 421 agagcatgtg gcaatactgg cggcggttgc cttgctgagt aaagcctttg gcgtaattgc 481 cgatgcagat ggcctcacgc cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc 541 catcggcatg caaggattgg ttcagggtca gttcaaggat ctgtctgaag gggataagcc 601 gcgcagcgct gaagctattt tgatgacgaa tcactttaaa accagcacgc tgttttgtgc 661 ctccatgcag atggcctcga ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca 721 tcgtttttca cttgatcttg gtcaggcatt tcaactgctg gacgatttga ccgatggcat 781 gaccgacacc ggtaaggata gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt 841 aggcccgagg gcggttgaag aacgtctgag acaacatctt cagcttgcca gtgagcatct 901 ctctgcggcc tgccaacacg ggcacgccac tcaacatttt attcaggcct ggtttgacaa 961 aaaactcgct gccgtcagtt aataatacta gagctcaagg aggtactaga tgaataatcc 1021 gtcgttactc aatcatgcgg tcgaaacgat ggcagttggc tcgaaaagtt ttgcgacagc 1081 ctcaaagtta tttgatgcaa aaacccggcg cagcgtactg atgctctacg cctggtgccg 1141 ccattgtgac gatgttattg acgatcagac gctgggcttt caggcccggc agcctgcctt 1201 acaaacgccc gaacaacgtc tgatgcaact tgagatgaaa acgcgccagg cctatgcagg 1261 atcgcagatg cacgaaccgg cgtttgcggc ttttcaggaa gtggctatgg ctcatgatat 1321 cgccccggct tacgcgtttg atcatctgga aggcttcgcc atggatgtac gcgaagcgca 1381 atacagccaa ctggatgata cgctgcgcta ttgctatcac gttgcaggcg ttgtcggctt 1441 gatgatggcg caaatcatgg gcgtgcggga taacgccacg ctggaccgcg cctgtgacct 1501 tgggctggca tttcagttga ccaatattgc tcgcgatatt gtggacgatg cgcatgcggg 1561 ccgctgttat ctgccggcaa gctggctgga gcatgaaggt ctgaacaaag agaattatgc 1621 ggcacctgaa aaccgtcagg cgctgagccg tatcgcccgt cgtttggtgc aggaagcaga 1681 accttactat ttgtctgcca cagccggcct ggcagggttg cccctgcgtt ccgcctgggc 1741 aatcgctacg gcgaagcagg tttaccggaa aataggtgtc aaagttgaac aggccggtca 1801 gcaagcctgg gatcagcggc agtcaacgac cacgcccgaa aaattaacgc tgctgctggc 1861 cgcctctggt caggccctta cttcccggat gcgggctcat cctccccgcc ctgcgcatct 1921 ctggcagcgc ccgctctaat aatactagag ctcaaggagg tactagatga aaccaactac 1981 ggtaattggt gcaggcttcg gtggcctggc actggcaatt cgtctacaag ctgcggggat 2041 ccccgtctta ctgcttgaac aacgtgataa acccggcggt cgggcttatg tctacgagga 2101 tcaggggttt acctttgatg caggcccgac ggttatcacc gatcccagtg ccattgaaga 2161 actgtttgca ctggcaggaa aacagttaaa agagtatgtc gaactgctgc cggttacgcc 2221 gttttaccgc ctgtgttggg agtcagggaa ggtctttaat tacgataacg atcaaacccg 2281 gctcgaagcg cagattcagc agtttaatcc ccgcgatgtc gaaggttatc gtcagtttct 2341 ggactattca cgcgcggtgt ttaaagaagg ctatctaaag ctcggtactg tccctttttt 2401 atcgttcaga gacatgcttc gcgccgcacc tcaactggcg aaactgcaag catggagaag 2461 cgtttacagt aaggttgcca gttacatcga agatgaacat ctgcgccagg cgttttcttt 2521 ccactcgctg ttggtgggcg gcaatccctt cgccacctca tccatttata cgttgataca 2581 cgcgctggag cgtgagtggg gcgtctggtt tccgcgtggc ggcaccggcg cattagttca 2641 ggggatgata aagctgtttc aggatctggg tggcgaagtc gtgttaaacg ccagagtcag 2701 ccatatggaa acgacaggaa acaagattga agccgtgcat ttagaggacg gtcgcaggtt 2761 cctgacgcaa gccgtcgcgt caaatgcaga tgtggttcat acctatcgcg acctgttaag 2821 ccagcaccct gccgcggtta agcagtccaa caaactgcaa actaagcgca tgagtaactc 2881 tctgtttgtg ctctattttg gtttgaatca ccatcatgat cagctcgcgc atcacacggt 2941 ttgtttcggc ccgcgttacc gcgagctgat tgacgaaatt tttaatcatg atggcctcgc 3001 agaggacttc tcactttatc tgcacgcgcc ctgtgtcacg gattcgtcac tggcgcctga 3061 aggttgcggc agttactatg tgttggcgcc ggtgccgcat ttaggcaccg cgaacctcga 3121 ctggacggtt gaggggccaa aactacgcga ccgtattttt gcgtaccttg agcagcatta 3181 catgcctggc ttacggagtc agctggtcac gcaccggatg tttacgccgt ttgattttcg 3241 cgaccagctt aatgcctatc atggctcagc cttttctgtg gagcccgttc ttacccagag 3301 cgcctggttt cggccgcata accgcgataa aaccattact aatctctacc tggtcggcgc 3361 aggcacgcat cccggcgcag gcattcctgg cgtcatcggc tcggcaaaag cgacagcagg 3421 tttgatgctg gaggatctga tataataata ctagaggagg tactagatgc aaccgcatta 3481 tgatctgatt ctcgtggggg ctggactcgc gaatggcctt atcgccctgc gtcttcagca 3541 gcagcaacct gatatgcgta ttttgcttat cgacgccgca ccccaggcgg gcgggaatca 3601 tacgtggtca tttcaccacg atgatttgac tgagagccaa catcgttgga tagctccgct 3661 ggtggttcat cactggcccg actatcaggt acgctttccc acacgccgtc gtaagctgaa 3721 cagcggctac ttttgtatta cttctcagcg tttcgctgag gttttacagc gacagtttgg 3781 cccgcacttg tggatggata ccgcggtcgc agaggttaat gcggaatctg ttcggttgaa 3841 aaagggtcag gttatcggtg cccgcgcggt gattgacggg cggggttatg cggcaaattc 3901 agcactgagc gtgggcttcc aggcgtttat tggccaggaa tggcgattga gccacccgca 3961 tggtttatcg tctcccatta tcatggatgc cacggtcgat cagcaaaatg gttatcgctt 4021 cgtgtacagc ctgccgctct cgccgaccag attgttaatt gaagacacgc actatattga 4081 taatgcgaca ttagatcctg aatgcgcgcg gcaaaatatt tgcgactatg ccgcgcaaca 4141 gggttggcag cttcagacac tgctgcgaga agaacagggc gccttaccca ttactctgtc 4201 gggcaatgcc gacgcattct ggcagcagcg ccccctggcc tgtagtggat tacgtgccgg 4261 tctgttccat cctaccaccg gctattcact gccgctggcg gttgccgtgg ccgaccgcct 4321 gagtgcactt gatgtcttta cgtcggcctc aattcaccat gccattacgc attttgcccg 4381 cgagcgctgg cagcagcagg gctttttccg catgctgaat cgcatgctgt ttttagccgg 4441 acccgccgat tcacgctggc gggttatgca gcgtttttat ggtttacctg aagatttaat 4501 tgcccgtttt tatgcgggaa aactcacgct gaccgatcgg ctacgtattc tgagcggcaa 4561 gccgcctgtt ccggtattag cagcattgca agccattatg acgactcatc gttaataata 4621 ctagaggaaa gaggagaaat actagaatga ttaccaccga tgttgttatt attggcgcag 4681 gtcataatgg tctggtttgt gcagcatatc tgctgcaacg tggtctgggt gttaccctgc 4741 tggaaaaacg tgaagttccg ggtggtgcag caaccaccga agcactgatg ccggaactga 4801 gtccgcagtt tcgttttaat cgttgtgcca ttgatcatga atttattttt ctgggtccgg 4861 ttctgcaaga actgaatctg gcacagtatg gtctggaata cctgttttgt gatccgagcg 4921 ttttttgtcc gggtctggat ggtcaggcat ttatgagcta tcgtagcctg gaaaaaacct 4981 gtgcacatat tgcaacctat agtccgcgtg atgcagaaaa atatcgccag tttgtgaatt 5041 attggaccga tctgctgaat gcagttcagc ctgcatttaa tgcacctccg caggcactgc 5101 tggatctggc actgaattat ggttgggaaa atctgaaaag cgttctggca attgcaggta 5161 gcaaaaccaa agcactggat tttattcgca ccatgattgg ttcaccggaa gatgttctga 5221 atgaatggtt tgatagcgaa cgtgttaaag caccgctggc acgtctgtgt agcgaaattg 5281 gtgcacctcc gagccagaaa ggtagcagca gcggtatgat gatggttgca atgcgtcatc 5341 tggaaggtat tgcacgtccg aaaggtggta caggtgcact gaccgaagcc ctggttaaac 5401 tggttcaggc acagggtggt aaaattctga ccgatcagac cgttaaacgt gttctggttg 5461 aaaataatca ggccattggt gttgaagttg caaatggtga acagtatcgt gccaaaaaag 5521 gcgtgattag caatattgat gcccgtcgtc tgtttctgca actggttgaa ccgggtgcac 5581 tggcaaaagt taatcagaat ctgggtgaac gtctggaacg tcgtaccgtt aataataatg 5641 aagccattct gaaaattgat tgtgcactga gcggtctgcc gcattttacc gcaatggcag 5701 gtcctgaaga tctgaccggc accattctga ttgcagatag cgttcgtcat gttgaagaag 5761 cacacgcact gattgcactg ggtcagattc cagatgcaaa tccgagcctg tatctggata 5821 ttccgaccgt tctggatccg accatggcac ctccgggtca gcataccctg tggattgaat 5881 tttttgcacc gtatcgtatt gcaggtctgg aaggtacagg tctgatgggt acaggttgga 5941 ccgatgaact gaaagaaaaa gttgccgatc gcgtgattga taaactgacc gattatgcac 6001 cgaatctgaa aagtctgatt attggtcgtc gtgttgaaag tccggcagaa ctggcacagc 6061 gtctgggtag ctataatggt aatgtttatc atctggatat gagcctggat cagatgatgt 6121 ttctgcgtcc gctgccggaa attgcaaatt atcagacccc gattaaaaat ctgtatctga 6181 caggtgcagg cacccatccg ggtggtagca ttagcggtat gccgggtcgt aattgtgcac 6241 gtgtttttct gaaacagcag cgtcgttttt ggtaa //