##gff-version 3
##sequence-region BBa_K323139 1 6830
BBa_K323139	.	engineered_region	1	5173	.	+	0	ID=BBa_K323122;Name=BBa_K323122
BBa_K323139	.	engineered_region	1	4618	.	+	0	ID=BBa_K274210;Name=BBa_K274210;Parent=BBa_K323122
BBa_K323139	.	ribosome_entry_site	994	1003	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274210
BBa_K323139	.	sequence_feature	994	996	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K323139	.	ribosome_entry_site	997	1003	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K323139	.	ribosome_entry_site	64	67	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274210
BBa_K323139	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K274210
BBa_K323139	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K323139	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K323139	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K323139	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K323139	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K323139	.	CDS	1967	3448	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K274210
BBa_K323139	.	point_mutation	2444	2449	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K323139	.	CDS	1967	3445	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_K323139	.	point_mutation	2855	2860	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K323139	.	CDS	74	985	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K274210
BBa_K323139	.	ribosome_entry_site	3457	3460	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274210
BBa_K323139	.	CDS	1010	1942	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K274210
BBa_K323139	.	CDS	1010	1939	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_K323139	.	CDS	3467	4618	.	+	0	ID=BBa_K118008;Name=BBa_K118008;Parent=BBa_K274210
BBa_K323139	.	CDS	3467	4615	.	+	0	ID=annotation1995716;Name=crtY coding sequence;Parent=BBa_K118008
BBa_K323139	.	ribosome_entry_site	1951	1960	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274210
BBa_K323139	.	sequence_feature	1951	1953	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K323139	.	ribosome_entry_site	1954	1960	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K323139	.	engineered_region	4627	5173	.	+	0	ID=BBa_I742158;Name=BBa_I742158;Parent=BBa_K323122
BBa_K323139	.	ribosome_entry_site	4627	4642	.	+	0	ID=BBa_I742156;Name=BBa_I742156;Parent=BBa_I742158
BBa_K323139	.	CDS	4643	5173	.	+	0	ID=BBa_I742157;Name=BBa_I742157;Parent=BBa_I742158
BBa_K323139	.	CDS	4643	5170	.	+	0	ID=annotation1953812;Name=crtZ;Parent=BBa_I742157
BBa_K323139	.	engineered_region	5182	6830	.	+	0	ID=BBa_K323213;Name=BBa_K323213
BBa_K323139	.	ribosome_entry_site	5187	5193	.	+	0	ID=annotation2100003;Name=Elowitz RBS;Parent=BBa_K323213
BBa_K323139	.	sequence_feature	5201	6830	.	+	0	ID=annotation2100004;Name=crtO;Parent=BBa_K323213
>BBa_K323139
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggaggtactagatgacg
gtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatc
agttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtat
tcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgt
gcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggac
gccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgt
aattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaa
ggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatc
actttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtga
ttgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacacc
ggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctga
gacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggc
ctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgtta
ctcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaaccc
ggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggc
ccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcg
cagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatc
atctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgt
tgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgacctt
gggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaa
gctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccg
tcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgg
gcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagc
ggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggc
tcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaacca
actacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgc
ttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggt
tatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccg
gttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgc
agattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaagg
ctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaa
gcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgc
tgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctg
gtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgtta
aacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctga
cgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagca
gtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcag
ctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcag
aggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgt
gttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtattttt
gcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttc
gcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgca
taaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatc
ggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataatactagaggaggtactagatgcaaccgc
attatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatat
gcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgag
agccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgta
agctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtg
gatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtg
attgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattga
gccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacag
cctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcg
cggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttac
ccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgtt
ccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcg
gcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgca
tgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgc
ccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagca
gcattgcaagccattatgacgactcatcgttaataatactagagctctaccggagaaattatgttgtggatttggaatg
ccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctgggg
ttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgct
gcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgt
atggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggcta
cctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctc
tatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgc
agggcggggaggatgagcccgcatccgggaagtaataatactagaggaaagaggagaaatactagaatgattaccaccg
atgttgttattattggcgcaggtcataatggtctggtttgtgcagcatatctgctgcaacgtggtctgggtgttaccct
gctggaaaaacgtgaagttccgggtggtgcagcaaccaccgaagcactgatgccggaactgagtccgcagtttcgtttt
aatcgttgtgccattgatcatgaatttatttttctgggtccggttctgcaagaactgaatctggcacagtatggtctgg
aatacctgttttgtgatccgagcgttttttgtccgggtctggatggtcaggcatttatgagctatcgtagcctggaaaa
aacctgtgcacatattgcaacctatagtccgcgtgatgcagaaaaatatcgccagtttgtgaattattggaccgatctg
ctgaatgcagttcagcctgcatttaatgcacctccgcaggcactgctggatctggcactgaattatggttgggaaaatc
tgaaaagcgttctggcaattgcaggtagcaaaaccaaagcactggattttattcgcaccatgattggttcaccggaaga
tgttctgaatgaatggtttgatagcgaacgtgttaaagcaccgctggcacgtctgtgtagcgaaattggtgcacctccg
agccagaaaggtagcagcagcggtatgatgatggttgcaatgcgtcatctggaaggtattgcacgtccgaaaggtggta
caggtgcactgaccgaagccctggttaaactggttcaggcacagggtggtaaaattctgaccgatcagaccgttaaacg
tgttctggttgaaaataatcaggccattggtgttgaagttgcaaatggtgaacagtatcgtgccaaaaaaggcgtgatt
agcaatattgatgcccgtcgtctgtttctgcaactggttgaaccgggtgcactggcaaaagttaatcagaatctgggtg
aacgtctggaacgtcgtaccgttaataataatgaagccattctgaaaattgattgtgcactgagcggtctgccgcattt
taccgcaatggcaggtcctgaagatctgaccggcaccattctgattgcagatagcgttcgtcatgttgaagaagcacac
gcactgattgcactgggtcagattccagatgcaaatccgagcctgtatctggatattccgaccgttctggatccgacca
tggcacctccgggtcagcataccctgtggattgaattttttgcaccgtatcgtattgcaggtctggaaggtacaggtct
gatgggtacaggttggaccgatgaactgaaagaaaaagttgccgatcgcgtgattgataaactgaccgattatgcaccg
aatctgaaaagtctgattattggtcgtcgtgttgaaagtccggcagaactggcacagcgtctgggtagctataatggta
atgtttatcatctggatatgagcctggatcagatgatgtttctgcgtccgctgccggaaattgcaaattatcagacccc
gattaaaaatctgtatctgacaggtgcaggcacccatccgggtggtagcattagcggtatgccgggtcgtaattgtgca
cgtgtttttctgaaacagcagcgtcgtttttggtaa