BBa_K324001 1 BBa_K324001 AraC regulatory protein in RFC25 format 2010-10-24T11:00:00Z 2015-05-08T01:12:02Z refer to http://partsregistry.org/Part:BBa_C0080 This part consists of the AraC regulatory protein optimized with endings for protein fusion in the Freiburg assembly format (RFC25). There is also a 6-his tag appended to the C-terminus for easy purification using Ni-affinity column. The LVA rapid degradation tag from our source DNA, BBa_C0080, was also removed from the C-terminus. All modifications were made to BBa_C0080 by means of PCR with specially designed primer set. false false _444_ 0 6047 9 It's complicated true The LVA tag was removed because this part's intended purpose, harvesting from cells and purification, required the protein to have a long lifespan. 6-his tag was added to the C-terminus to avoid isolation of incompletely translated peptides during Ni-affinity purification. false Julius Ho annotation2100703 1 AraC in RFC25 range2100703 1 1 924 BBa_K324001_sequence 1 atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctcatcatcatcatcatcattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z