LOCUS BBa_K324050 3277 bp DNA linear 29-Apr-2024 DEFINITION TrpR-RBS-TetR-Term-pTetR-RBS-CI-RBS-AraC-RBS-Mnt-Term ACCESSION BBa_K324050 VERSION BBa_K324050.1 FEATURES Location/Qualifiers promoter 1..85 /label=BBa_K191007 misc_feature 98..101 /label=conserved RBS 94..105 /label=BBa_B0034 CDS 115..731 /label=tetR misc_feature 732..765 /label=SsrA misc_feature 772..796 /label=Help:Barcodes CDS 112..796 /label=BBa_C0040 stem_loop 816..859 /label=stem_loop misc_feature 805..884 /label=BBa_B0010 terminator 805..884 /label=BBa_B0010 stem_loop 904..947 /label=stem_loop misc_feature 893..972 /label=BBa_B0010 terminator 893..972 /label=BBa_B0010 misc_feature 981..999 /label=TetR 1 misc_feature 981..1034 /label=BBa_R0040 promoter 1000..1005 /label=-35 misc_feature 1006..1024 /label=TetR 2 promoter 1023..1028 /label=-10 promoter 981..1034 /label=BBa_R0040 misc_feature 1047..1050 /label=conserved RBS 1043..1054 /label=BBa_B0034 CDS 1064..1771 /label=cI lambda misc_feature 1772..1804 /label=LVA misc_feature 1811..1835 /label=Help:Barcodes CDS 1061..1835 /label=BBa_C0051 misc_feature 1848..1851 /label=conserved RBS 1844..1855 /label=BBa_B0034 CDS 1862..2737 /label=araC misc_feature 2738..2770 /label=LVA misc_feature 2777..2801 /label=Help:Barcodes CDS 1862..2801 /label=BBa_C0080 misc_feature 2814..2817 /label=conserved RBS 2810..2821 /label=BBa_B0034 CDS 2828..3076 /label=mnt misc_feature 2922..2924 /label=T misc_feature 3077..3109 /label=LVA misc_feature 3116..3140 /label=Help:Barcodes CDS 2828..3115 /label=BBa_C0072 misc_feature 2810..3140 /label=BBa_I13025 stem_loop 3160..3203 /label=stem_loop misc_feature 3149..3228 /label=BBa_B0010 terminator 3149..3228 /label=BBa_B0010 stem_loop 3244..3263 /label=T7 TE misc_feature 3270..3270 /label=stop polyA_site 3264..3277 /label=polya misc_feature 3237..3277 /label=BBa_B0012 terminator 3237..3277 /label=BBa_B0012 terminator 3149..3277 /label=BBa_B0015 misc_feature 2810..3277 /label=BBa_K091141 ORIGIN 1 tggcaaatat tctgaaatga gctgttgaca attaatcatc gaactagtta actagtacgc 61 aagttcacgt aaaaagggta tcgactacta gagaaagagg agaaatacta gatgtccaga 121 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa 181 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg 241 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac 301 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct 361 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca 421 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa 481 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt 541 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact 601 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag 661 ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt 721 gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag 781 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 841 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agccaggcat 901 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 961 gtgaacgctc tctactagag tccctatcag tgatagagat tgacatccct atcagtgata 1021 gagatactga gcactactag agaaagagga gaaatactag atgagcacaa aaaagaaacc 1081 attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa 1141 aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg 1201 cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc 1261 aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga 1321 gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt 1381 ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc 1441 ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga 1501 aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat 1561 tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg 1621 tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact 1681 aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat 1741 cgctagtcag tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt 1801 agcttaataa cgctgatagt gctagtgtag atcgctacta gagaaagagg agaaatacta 1861 gatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaacg cccatctggt 1921 ggcgggttta acgccgattg aggccaatgg ttatctcgat ttttttatcg accgaccgct 1981 gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg tgaaaaatca 2041 gggacgagaa tttgtctgcc gaccgggtga tattttgctg ttcccgccag gagagattca 2101 tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt actttcgtcc 2161 gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata cgggtttctt 2221 tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa tcattaacgc 2281 cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg agcaattgtt 2341 actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata atcgggtacg 2401 cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata tcgccagcgt 2461 cgcacagcat gtttgcctgt cgccgtcgcg tctgtcacat cttttccgcc agcagttagg 2521 gattagcgtc ttaagctggc gcgaggacca acgcatcagc caggcgaagc tgcttttgag 2581 cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg atcaactcta 2641 tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc gtgccggttg 2701 tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcagct gcaaacgacg aaaactacgc 2761 tttagtagct taataacact gatagtgcta gtgtagatca ctactagaga aagaggagaa 2821 atactagatg gcccgggatg atcctcactt caattttcgt atgccaatgg aagtaagaga 2881 gaaattgaaa tttagagcag aggcaaacgg acggagcatg aactctgagc ttttgcaaat 2941 cgtacaagat gccctaagca aaccgtcacc agtcactggg taccgcaatg atgcggaacg 3001 actcgccgat gagcagagcg agttagtgaa gaagatggtc ttcgatacac tgaaggatct 3061 ttataaaaaa accaccgctg caaacgacga aaactacgct ttagtagctt aataaccctg 3121 atagtgctag tgtagatccc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3181 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3241 actggctcac cttcgggtgg gcctttctgc gtttata //