##gff-version 3
##sequence-region BBa_K324050 1 3277
BBa_K324050	.	promoter	981	1034	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K324050	.	engineered_region	981	1034	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K324050	.	non_covalent_binding_site	981	999	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K324050	.	non_covalent_binding_site	1006	1024	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K324050	.	promoter	1000	1005	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K324050	.	promoter	1023	1028	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K324050	.	CDS	1061	1835	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K324050	.	CDS	1064	1771	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K324050	.	tag	1772	1804	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K324050	.	engineered_tag	1811	1835	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K324050	.	ribosome_entry_site	1844	1855	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K324050	.	conserved_region	1848	1851	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K324050	.	ribosome_entry_site	1043	1054	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K324050	.	conserved_region	1047	1050	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K324050	.	engineered_region	2810	3277	.	+	0	ID=BBa_K091141;Name=BBa_K091141
BBa_K324050	.	engineered_region	2810	3140	.	+	0	ID=BBa_I13025;Name=BBa_I13025;Parent=BBa_K091141
BBa_K324050	.	CDS	2828	3115	.	+	0	ID=BBa_C0072;Name=BBa_C0072;Parent=BBa_I13025
BBa_K324050	.	point_mutation	2922	2924	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K324050	.	CDS	2828	3076	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K324050	.	tag	3077	3109	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K324050	.	engineered_tag	3116	3140	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_K324050	.	ribosome_entry_site	2810	2821	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13025
BBa_K324050	.	conserved_region	2814	2817	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K324050	.	terminator	3149	3277	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091141
BBa_K324050	.	terminator	3237	3277	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K324050	.	polyA_site	3264	3277	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K324050	.	engineered_region	3237	3277	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K324050	.	stem_loop	3244	3263	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K324050	.	stop_codon	3270	3270	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K324050	.	terminator	3149	3228	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K324050	.	stem_loop	3160	3203	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K324050	.	engineered_region	3149	3228	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K324050	.	CDS	1862	2801	.	+	0	ID=BBa_C0080;Name=BBa_C0080
BBa_K324050	.	CDS	1862	2737	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_K324050	.	engineered_tag	2777	2801	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_K324050	.	tag	2738	2770	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_K324050	.	ribosome_entry_site	94	105	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K324050	.	conserved_region	98	101	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K324050	.	promoter	1	85	.	+	0	ID=BBa_K191007;Name=BBa_K191007
BBa_K324050	.	terminator	893	972	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K324050	.	stem_loop	904	947	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K324050	.	engineered_region	893	972	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K324050	.	CDS	112	796	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K324050	.	tag	732	765	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K324050	.	engineered_tag	772	796	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K324050	.	CDS	115	731	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K324050	.	terminator	805	884	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K324050	.	stem_loop	816	859	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K324050	.	engineered_region	805	884	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K324050
tggcaaatattctgaaatgagctgttgacaattaatcatcgaactagttaactagtacgcaagttcacgtaaaaagggt
atcgactactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagct
gcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtat
tggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgcc
ctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcga
tggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgc
caacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaag
atcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctat
cgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaa
cttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgta
gatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatac
tgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacg
cacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggg
gatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaa
attctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgc
agccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaac
ctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaa
ggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcagg
ctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcgg
tcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagtt
atcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgat
agtgctagtgtagatcgctactagagaaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccggg
atactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccga
ccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttg
tctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatg
gtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacg
ggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaagggg
aagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtc
gctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgat
atcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcg
tcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgt
cggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgag
ttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttag
tagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggcccgggatgatcc
tcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaac
tctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgac
tcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgc
aaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata