LOCUS       BBa_K324052             1419 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac/Mnt - RBS - GAL4 - ECFP - Term
ACCESSION   BBa_K324052
VERSION     BBa_K324052.1  
FEATURES             Location/Qualifiers
     promoter        47..52
                     /label=-10
     misc_feature    53..87
                     /label=LacI
     misc_feature    1..52
                     /label=Mnt
     promoter        1..87
                     /label=BBa_K091104
     misc_feature    100..103
                     /label=conserved
     RBS             96..107
                     /label=BBa_B0034
     misc_feature    116..553
                     /label=BBa_K105007
     CDS             560..1282
                     /label=BBa_E0020
     stem_loop       1302..1345
                     /label=stem_loop
     misc_feature    1291..1370
                     /label=BBa_B0010
     terminator      1291..1370
                     /label=BBa_B0010
     stem_loop       1386..1405
                     /label=T7 TE
     misc_feature    1412..1412
                     /label=stop
     polyA_site      1406..1419
                     /label=polya
     misc_feature    1379..1419
                     /label=BBa_B0012
     terminator      1379..1419
                     /label=BBa_B0012
ORIGIN
        1 ctcgaggtga gtgcacagta ctaggtccac ggtgacctag atctcctata gttgtgtgga
       61 attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac tagagaagct
      121 actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa
      181 agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa
      241 aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag
      301 actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat
      361 ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa
      421 taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc taacattgag
      481 acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca
      541 gttgactgta tcgtactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      601 catcctggtc gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg
      661 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      721 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg
      781 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      841 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      901 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      961 cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg tctatatcac
     1021 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     1081 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1141 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1201 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1261 ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga aaggctcagt
     1321 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1381 acactggctc accttcgggt gggcctttct gcgtttata
//