LOCUS BBa_K325001 1710 bp DNA linear 25-Nov-2024 DEFINITION Mexican wave simulator ACCESSION BBa_K325001 VERSION BBa_K325001.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1647..1678 /label=LVA CDS 1068..1646 /label=luxI misc_feature 1068..1685 /label=BBa_C0061 misc_feature 1686..1710 /label=Help:Barcodes CDS 1068..1685 /label=BBa_C0061 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagatg actataatga 1081 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc 1141 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat 1201 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg 1261 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc 1321 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt 1381 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac 1441 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa 1501 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag 1561 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg 1621 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt 1681 aataactctg atagtgctag tgtagatctc //