LOCUS BBa_K325100 2626 bp DNA linear 22-Dec-2024 DEFINITION EPIC Firefly Luciferase and LRE
P. Pyralis
(E. coli optimised) ACCESSION BBa_K325100 VERSION BBa_K325100.1 FEATURES Location/Qualifiers RBS 1..17 /label=rbs CDS 18..941 /label=Luciferin regenerating enzyme (P.pyralis) misc_feature 942..947 /label=double stop codon misc_feature 948..953 /label=BamH1 site RBS 954..970 /label=rbs misc_feature 971..2620 /label=EPIC luciferase (P. pyralis) misc_feature 2621..2626 /label=double stop codon ORIGIN 1 ttaaggaggt aaaaaaaatg ggtccggtcg ttgaaaagat cgccgagctg ggtaagtaca 61 cggttggtga gggtccgcac tgggaccacg aaacgcagac gctgtatttc gtggatacgg 121 tcgagaaaac gttccacaaa tacgttccga gccagaagaa atacacgttt tgtaaggtgg 181 acaagctggt tagctttatc attccgctgg caggttctcc gggtcgcttc gtcgtcagct 241 tggagcgcga gattgcgatc ctgacgtggg acggcgtcag cgcggcaccg accagcattg 301 aggcgattgt gaatgtcgag ccgcacatca agaataatcg cctgaacgac ggtaaagcgg 361 acccgctggg taatctgtgg accggcacga tggccattga tgcgggtttg ccggtgggtc 421 cggtgaccgg ctccctgtac cacctgggtg cggataagaa ggtcaagatg catgaaagca 481 atattgccat cgctaacggc ctggcgtgga gcaacgacct gaagaagatg tactatattg 541 acagcggcaa acgtcgtgtt gacgaatacg actatgatgc gagcacgctg tctatcagca 601 atcagcgtcc gctgttcacc ttcgaaaaac atgaggtccc tggctaccca gacggtcaaa 661 ccattgacga agaaggtaac ctgtgggtcg ccgtgtttca aggccagcgt attatcaaga 721 ttagcaccca gcagccggag gttctgctgg ataccgtcaa aatcccggac ccgcaggtga 781 ctagcgttgc attcggtggt ccgaatctgg acgagctgca tgtgaccagc gccggtttgc 841 agctggatga cagctccctg gacaaatctt tggtcaatgg tcacgtctac cgtgtgactg 901 gcctgggtgt taagggtttc gccggtgtga aagttaagct gtaataagga tccttaagga 961 ggtaaaaaaa atggaggacg cgaaaaacat caagaaaggc ccagcaccgt tctatccgct 1021 ggaagatggt accgccggtg aacaattgca caaagcgatg aaacgttatg cgctggttcc 1081 gggtaccatc gcattcacgg acgcgcatat cgaggttaac atcacctatg ccgagtactt 1141 cgagatgagc gttcgtctgg ccgaggcaat gaagcgttat ggtttgaata ccaatcatcg 1201 catcgtcgtc tgtagcgaga acagcttgca gtttttcatg ccagttctgg gtgcactgtt 1261 tattggtgtg gcagtggcgc ctgcgaatga tatctacaac gagcgtgaac tgttgaatag 1321 catgaacatt tcgcagccga cggttgtttt tgtgtccaag aaaggtttgc agaagattct 1381 gaatgtgcaa aagaagttgc cgatcattca gaaaatcatc atcatggaca gcaagaccga 1441 ttaccagggt ttccaaagca tgtacacttt tgtgacgagc cacctgcctc cgggttttaa 1501 cgagtatgat ttcgttccgg aaagcttcga ccgtgataag acgattgcac tgattatgaa 1561 ctcgtccggc tctaccggcc tgcctaaagg cgtggccctg ccgcaccgta ctgcatgtgt 1621 ccgcttcagc cacgcgcgtg atccgatctt cggtaaccaa atcattccgg acaccgccat 1681 tttgtctgtc gttccgtttc atcatggctt tggtatgttt accactctgg gttacctgat 1741 ctgtggtttc cgtgttgttc tgatgtaccg tttcgaagaa gaactgttcc tgcgtagcct 1801 gcaagattac aagatccaat ccgcgctgct ggtgccgacg ctgtttagct tcttcgctaa 1861 atccaccctg attgacaaat atgacttgag caacctgcac gaaatcgcgt cgggcggtgc 1921 gccgctgagc aaagaagtcg gtgaggccgt tgcgaaacgt ttccatctgc cgggcatccg 1981 tcagggttat ggcctgacgg aaacgaccag cgcgattttg attacccctg agggtgacga 2041 taaaccgggt gccgttggca aggtggtacc gttctttgag gctaaagtcg tggacctgga 2101 cacgggtaag actctgggtg tgaatcaacg tggcgagttg tgcgttcgtg gcccgatgat 2161 catgagcggt tatgtcaaca acccggaagc aactaatgcg ttgatcgata aggatggctg 2221 gctgcacagc ggcgacctgg cgtactggga tgaggatgaa cactttttca tcgttggtcg 2281 cctgaagagc ctgatcaagt acaagggcta ccaggtggca ccggctgaac tggagagcat 2341 tctgttgcaa caccctaata tcttcgacgc gggtgttgcg ggtctgcctg atgatgatgc 2401 cggtgaattg ccagctgcgg tggtcgttct ggaacacggt aaaacgatga ctgaaaaaga 2461 aatcgtggac tacgtcgcct cgcaggtgac caccgctaaa aagttgcgtg gtggcgtggt 2521 gtttgtggat gaagtgccga aaggcctgac gggtaaacgt gatgcacgta agattcgtga 2581 gatcttgatc aaggcgaaga aaggtggtaa atccaagttg taataa //